breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 22 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG42.6% 479.5 / 92.0 312intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123063,527,3680AC32.4% 1307.2 / 159.9 603L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123061,944,2880AC30.3% 576.6 / 59.1 257G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP012306341,3770TG30.1% 279.8 / 24.4 153G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP012306644,4170GA29.5% 1092.9 / inf 662intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123062,758,9090TG26.9% 656.1 / 25.4 257G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123063,938,8870CT25.9% 571.1 / 140.7 263S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123061,226,5570AG25.6% 441.1 / 81.2 203G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123061,854,8510AC25.2% 804.2 / 75.2 306T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123063,372,7010TG25.0% 216.3 / 12.2 96noncoding (54/76 nt)gltUtRNA‑Glu
*AP0123061,309,9140AC24.7% 400.3 / 13.8 174L23F (TTA→TTClsrCAI2 transporter
*AP0123063,903,7910AG24.0% 668.7 / 70.8 279E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123061,128,4120TG23.1% 986.0 / 51.2 370V29G (GTG→GGG) yciOconserved protein
*AP0123063,324,4330TA22.9% 731.5 / 113.6 288intergenic (+2/+52)yieF/yieGchromate reductase, Class I, flavoprotein/predicted inner membrane protein
*AP012306800,6750AC22.8% 727.4 / 25.8 281T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123061,074,6190TC21.6% 492.6 / 121.2 255S29G (AGC→GGC) ldrAtoxic polypeptide, small
*AP012306524,2890TG21.0% 513.0 / 12.9 186N4T (AAC→ACC) citDcitrate lyase, acyl carrier (gamma) subunit
*AP0123063,412,0490TG20.8% 1084.8 / 41.3 405intergenic (+25/‑122)proM/aslBtRNA‑Pro/predicted regulator of arylsulfatase activity
*AP012306257,1370TG20.5% 539.7 / 11.2 221L496V (TTG→GTG) betTcholine transporter of high affinity
*AP012306935,0540GT20.4% 273.6 / 36.3 93intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein

Marginal new junction evidence (lowest skew 10 of 90 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 997497388 (1.000)15 (0.040) 15/268 10.8 4.2% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 340 (0.980)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 1092334385 (0.990)14 (0.040) 11/258 11.7 4.1% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 317 (0.950)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 1092512464 (1.200)16 (0.050) 8/258 12.7 4.3% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 318 (0.950)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 186236 =400 (1.030)10 (0.030) 9/290 12.9 2.5% coding (830/1353 nt) yaeL zinc metallopeptidase
?AP012306 186261 = 386 (1.020)coding (855/1353 nt) yaeL zinc metallopeptidase
* ? AP012306 1856463 =303 (0.780)7 (0.020) 7/274 13.4 2.5% coding (1685/1761 nt) yejH predicted ATP‑dependet helicase
?AP012306 1856475 = 279 (0.780)coding (1697/1761 nt) yejH predicted ATP‑dependet helicase
* ? AP012306 1630511 =351 (0.910)6 (0.020) 6/274 13.8 1.8% coding (158/1773 nt) aspS aspartyl‑tRNA synthetase
?AP012306 1630523 = 328 (0.920)coding (146/1773 nt) aspS aspartyl‑tRNA synthetase
* ? AP012306 = 1831476387 (1.000)6 (0.020) 6/284 14.0 1.6% coding (7/1251 nt) yeiM predicted nucleoside transporter
?AP012306 = 1831480 371 (1.000)coding (3/1251 nt) yeiM predicted nucleoside transporter
* ? AP012306 = 215364NA (NA)6 (0.020) 6/288 14.0 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 497268 =323 (0.830)6 (0.020) 6/292 14.1 1.9% coding (759/957 nt) fepB iron‑enterobactin transporter subunit
?AP012306 497289 = 318 (0.840)coding (738/957 nt) fepB iron‑enterobactin transporter subunit
* ? AP012306 = 42209318 (0.820)5 (0.010) 5/270 14.2 1.7% coding (139/531 nt) kefF flavoprotein subunit for the KefC potassium efflux system
?AP012306 = 42195 290 (0.830)coding (125/531 nt) kefF flavoprotein subunit for the KefC potassium efflux system