breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 22 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 1,091,992 | 0 | T | G | 42.6% | 479.5 / 92.0 | 312 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 3,527,368 | 0 | A | C | 32.4% | 1307.2 / 159.9 | 603 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 1,944,288 | 0 | A | C | 30.3% | 576.6 / 59.1 | 257 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 341,377 | 0 | T | G | 30.1% | 279.8 / 24.4 | 153 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
* | AP012306 | 644,417 | 0 | G | A | 29.5% | 1092.9 / inf | 662 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 2,758,909 | 0 | T | G | 26.9% | 656.1 / 25.4 | 257 | G33G (GGT→GGG) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
* | AP012306 | 3,938,887 | 0 | C | T | 25.9% | 571.1 / 140.7 | 263 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 1,226,557 | 0 | A | G | 25.6% | 441.1 / 81.2 | 203 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | AP012306 | 1,854,851 | 0 | A | C | 25.2% | 804.2 / 75.2 | 306 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 3,372,701 | 0 | T | G | 25.0% | 216.3 / 12.2 | 96 | noncoding (54/76 nt) | gltU | tRNA‑Glu |
* | AP012306 | 1,309,914 | 0 | A | C | 24.7% | 400.3 / 13.8 | 174 | L23F (TTA→TTC) | lsrC | AI2 transporter |
* | AP012306 | 3,903,791 | 0 | A | G | 24.0% | 668.7 / 70.8 | 279 | E189G (GAA→GGA) | yjgJ | predicted transcriptional regulator |
* | AP012306 | 1,128,412 | 0 | T | G | 23.1% | 986.0 / 51.2 | 370 | V29G (GTG→GGG) | yciO | conserved protein |
* | AP012306 | 3,324,433 | 0 | T | A | 22.9% | 731.5 / 113.6 | 288 | intergenic (+2/+52) | yieF/yieG | chromate reductase, Class I, flavoprotein/predicted inner membrane protein |
* | AP012306 | 800,675 | 0 | A | C | 22.8% | 727.4 / 25.8 | 281 | T192P (ACC→CCC) | lolA | chaperone for lipoproteins |
* | AP012306 | 1,074,619 | 0 | T | C | 21.6% | 492.6 / 121.2 | 255 | S29G (AGC→GGC) | ldrA | toxic polypeptide, small |
* | AP012306 | 524,289 | 0 | T | G | 21.0% | 513.0 / 12.9 | 186 | N4T (AAC→ACC) | citD | citrate lyase, acyl carrier (gamma) subunit |
* | AP012306 | 3,412,049 | 0 | T | G | 20.8% | 1084.8 / 41.3 | 405 | intergenic (+25/‑122) | proM/aslB | tRNA‑Pro/predicted regulator of arylsulfatase activity |
* | AP012306 | 257,137 | 0 | T | G | 20.5% | 539.7 / 11.2 | 221 | L496V (TTG→GTG) | betT | choline transporter of high affinity |
* | AP012306 | 935,054 | 0 | G | T | 20.4% | 273.6 / 36.3 | 93 | intergenic (+24/‑1406) | putP/ycdO | proline:sodium symporter/conserved protein |
Marginal new junction evidence (lowest skew 10 of 90 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | = 997497 | 388 (1.000) | 15 (0.040) | 15/268 | 10.8 | 4.2% | coding (41/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase |
? | AP012306 | 997993 = | 340 (0.980) | coding (537/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase | |||||
* | ? | AP012306 | = 1092334 | 385 (0.990) | 14 (0.040) | 11/258 | 11.7 | 4.1% | coding (198/276 nt) | ychS | predicted protein |
? | AP012306 | 1092985 = | 317 (0.950) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | = 1092512 | 464 (1.200) | 16 (0.050) | 8/258 | 12.7 | 4.3% | intergenic (+100/+3) | ychS/tpr | predicted protein/predicted protamine‑like protein |
? | AP012306 | 1092985 = | 318 (0.950) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | 186236 = | 400 (1.030) | 10 (0.030) | 9/290 | 12.9 | 2.5% | coding (830/1353 nt) | yaeL | zinc metallopeptidase |
? | AP012306 | 186261 = | 386 (1.020) | coding (855/1353 nt) | yaeL | zinc metallopeptidase | |||||
* | ? | AP012306 | 1856463 = | 303 (0.780) | 7 (0.020) | 7/274 | 13.4 | 2.5% | coding (1685/1761 nt) | yejH | predicted ATP‑dependet helicase |
? | AP012306 | 1856475 = | 279 (0.780) | coding (1697/1761 nt) | yejH | predicted ATP‑dependet helicase | |||||
* | ? | AP012306 | 1630511 = | 351 (0.910) | 6 (0.020) | 6/274 | 13.8 | 1.8% | coding (158/1773 nt) | aspS | aspartyl‑tRNA synthetase |
? | AP012306 | 1630523 = | 328 (0.920) | coding (146/1773 nt) | aspS | aspartyl‑tRNA synthetase | |||||
* | ? | AP012306 | = 1831476 | 387 (1.000) | 6 (0.020) | 6/284 | 14.0 | 1.6% | coding (7/1251 nt) | yeiM | predicted nucleoside transporter |
? | AP012306 | = 1831480 | 371 (1.000) | coding (3/1251 nt) | yeiM | predicted nucleoside transporter | |||||
* | ? | AP012306 | = 215364 | NA (NA) | 6 (0.020) | 6/288 | 14.0 | NA | noncoding (745/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) |
? | AP012306 | = 215372 | NA (NA) | noncoding (753/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) | |||||
* | ? | AP012306 | 497268 = | 323 (0.830) | 6 (0.020) | 6/292 | 14.1 | 1.9% | coding (759/957 nt) | fepB | iron‑enterobactin transporter subunit |
? | AP012306 | 497289 = | 318 (0.840) | coding (738/957 nt) | fepB | iron‑enterobactin transporter subunit | |||||
* | ? | AP012306 | = 42209 | 318 (0.820) | 5 (0.010) | 5/270 | 14.2 | 1.7% | coding (139/531 nt) | kefF | flavoprotein subunit for the KefC potassium efflux system |
? | AP012306 | = 42195 | 290 (0.830) | coding (125/531 nt) | kefF | flavoprotein subunit for the KefC potassium efflux system |