breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG40.6% 433.9 / 66.2 251intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP012306341,3770TG32.6% 201.8 / 28.2 129G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123061,944,2880AC31.8% 531.0 / 55.7 245G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP012306644,4170GA30.5% 704.4 / inf 459intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123061,421,1470TG28.9% 396.6 / 10.2 197T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
*AP0123063,527,3680AC28.7% 1034.5 / 97.6 428L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123063,938,8870CT27.6% 445.1 / 124.6 221S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123061,309,9140AC27.6% 227.8 / 10.1 116L23F (TTA→TTClsrCAI2 transporter
*AP012306800,6750AC26.2% 582.1 / 34.1 240T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123061,854,8510AC25.6% 702.1 / 58.8 274T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123061,226,5570AG25.0% 428.5 / 70.1 188G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123063,412,0490TG23.6% 1641.8 / 68.9 646intergenic (+25/‑122)proM/aslBtRNA‑Pro/predicted regulator of arylsulfatase activity
*AP0123063,324,4330TA23.4% 603.0 / 87.2 248intergenic (+2/+52)yieF/yieGchromate reductase, Class I, flavoprotein/predicted inner membrane protein
*AP0123063,182,8610AC23.3% 370.5 / 12.3 134L198F (TTA→TTCyiaNpredicted transporter
*AP0123063,590,3670AC23.3% 1346.3 / 59.1 486T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
*AP012306935,0540GT23.2% 245.3 / 38.3 82intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123062,857,9110AC22.8% 577.4 / 15.4 202K180N (AAA→AACaaeRpredicted DNA‑binding transcriptional regulator, efflux system
*AP0123061,128,4120TG22.6% 712.2 / 28.0 265V29G (GTG→GGG) yciOconserved protein
*AP0123063,903,7910AG21.8% 562.0 / 56.8 226E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123062,714,5550TG21.6% 709.1 / 17.3 270T175P (ACC→CCC) ygjVconserved inner membrane protein

Marginal new junction evidence (lowest skew 10 of 102 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 1092512359 (1.120)20 (0.070) 13/258 15.4 6.7% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 244 (0.880)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 997497277 (0.860)14 (0.050) 14/268 15.4 5.2% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 263 (0.910)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 215364NA (NA)12 (0.040) 12/288 16.7 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 1092334294 (0.910)10 (0.040) 8/258 17.6 3.9% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 244 (0.880)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 3528620602 (1.870)7 (0.020) 7/294 19.1 1.2% coding (852/939 nt) rhaR DNA‑binding transcriptional activator, L‑rhamnose‑binding
?AP012306 = 3528633 587 (1.850)coding (865/939 nt) rhaR DNA‑binding transcriptional activator, L‑rhamnose‑binding
* ? AP012306 = 1341258291 (0.900)6 (0.020) 6/294 19.6 2.1% coding (1607/2427 nt) ynfE oxidoreductase subunit
?AP012306 = 1341274 280 (0.880)coding (1623/2427 nt) ynfE oxidoreductase subunit
* ? AP012306 = 1211471305 (0.950)6 (0.020) 6/292 19.6 1.9% intergenic (+34/‑29) ydcH/rimL predicted protein/ribosomal‑protein‑L7/L12‑serine acetyltransferase
?AP012306 = 1211495 316 (1.000)intergenic (+58/‑5) ydcH/rimL predicted protein/ribosomal‑protein‑L7/L12‑serine acetyltransferase
* ? AP012306 3606863 =365 (1.130)5 (0.020) 5/284 19.9 1.4% coding (293/384 nt) secE preprotein translocase membrane subunit
?AP012306 3606878 = 341 (1.110)coding (308/384 nt) secE preprotein translocase membrane subunit
* ? AP012306 = 2308127364 (1.130)5 (0.020) 5/284 19.9 1.4% coding (73/525 nt) ygaP predicted inner membrane protein with hydrolase activity
?AP012306 = 2308138 357 (1.160)coding (84/525 nt) ygaP predicted inner membrane protein with hydrolase activity
* ? AP012306 = 2275071207 (0.640)48 (0.170)
+GTATCAGTCTGCTTCGCAAG
4/258 19.9 17.4% noncoding (1/76 nt) gltW tRNA‑Glu
?AP012306 = 3597478 321 (1.000)intergenic (+65/‑107) rrsB/gltT 16S ribosomal RNA (rrnB)/tRNA‑Glu