breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 32 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG38.8% 549.5 / 97.0 318intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123063,938,8870CT33.9% 405.4 / 187.5 248S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP012306644,4170GA33.6% 820.6 / inf 608intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123061,944,2880AC32.8% 530.9 / 66.4 262G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123061,066,3230AC31.8% 547.9 / 42.6 243intergenic (‑92/+33)ychM/prsApredicted transporter/phosphoribosylpyrophosphate synthase
*AP012306341,3770TG30.6% 252.7 / 21.9 147G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123061,421,1470TG29.5% 489.3 / 14.8 238T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
*AP0123063,527,3680AC28.8% 769.1 / 71.4 327L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123062,758,9090TG28.8% 654.2 / 34.7 271G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP012306257,1370TG28.6% 410.3 / 13.6 204L496V (TTG→GTG) betTcholine transporter of high affinity
*AP0123062,212,7530AC28.2% 431.3 / 59.2 188Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP012306800,6750AC27.2% 668.5 / 38.4 291T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP012306406,6770AG27.0% 462.0 / 28.9 200K294E (AAA→GAA) hemHferrochelatase
*AP0123061,854,8510AC26.3% 646.5 / 78.0 274T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123061,226,5570AG25.9% 459.8 / 98.4 212G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123062,558,0710AC24.5% 710.2 / 36.6 282V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP0123063,903,7910AG23.9% 641.4 / 73.3 272E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123063,590,3670AC23.5% 891.5 / 37.0 328T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
*AP012306202,4170TG23.2% 829.6 / 26.5 326V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123063,372,7010TG23.2% 186.5 / 13.4 82noncoding (54/76 nt)gltUtRNA‑Glu

Marginal new junction evidence (lowest skew 10 of 53 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 997497368 (1.020)11 (0.030) 11/268 15.6 3.2% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 341 (1.050)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 1092334323 (0.890)14 (0.040) 10/258 15.7 4.5% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 319 (1.020)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 1092512442 (1.220)16 (0.050) 9/258 16.2 4.4% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 319 (1.020)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 2094536310 (0.860)8 (0.020) 8/274 17.0 2.7% coding (394/834 nt) cysU sulfate/thiosulfate transporter subunit
?AP012306 = 2094524 285 (0.860)coding (406/834 nt) cysU sulfate/thiosulfate transporter subunit
* ? AP012306 3604993 =409 (1.130)6 (0.020) 6/284 18.2 1.5% noncoding (27/76 nt) thrT tRNA‑Thr
?AP012306 3605010 = 389 (1.130)noncoding (44/76 nt) thrT tRNA‑Thr
* ? AP012306 2650038 =525 (1.450)6 (0.020) 6/292 18.4 1.2% intergenic (‑113/‑39) ygiW/qseB conserved protein/DNA‑binding response regulator in two‑component regulatory system with QseC
?AP012306 2650053 = 503 (1.420)intergenic (‑128/‑24) ygiW/qseB conserved protein/DNA‑binding response regulator in two‑component regulatory system with QseC
* ? AP012306 = 2125209362 (1.000)5 (0.020) 5/274 18.5 1.5% coding (917/951 nt) talA transaldolase A
?AP012306 = 2125197 338 (1.020)coding (905/951 nt) talA transaldolase A
* ? AP012306 = 2275071218 (0.600)51 (0.160)
+GTATCAGTCTGCTTCGCAAG
4/258 18.8 15.6% noncoding (1/76 nt) gltW tRNA‑Glu
?AP012306 = 3597478 420 (1.160)intergenic (+65/‑107) rrsB/gltT 16S ribosomal RNA (rrnB)/tRNA‑Glu
* ? AP012306 955967 =328 (0.910)5 (0.010) 5/292 18.9 1.5% intergenic (+2096/‑575) mdoG/yceK glucan biosynthesis protein, periplasmic/predicted lipoprotein
?AP012306 955990 = 332 (0.940)intergenic (+2119/‑552) mdoG/yceK glucan biosynthesis protein, periplasmic/predicted lipoprotein
* ? AP012306 854835 =293 (0.810)5 (0.010) 5/290 18.9 1.7% coding (1481/2613 nt) pepN aminopeptidase N
?AP012306 854853 = 286 (0.810)coding (1499/2613 nt) pepN aminopeptidase N