breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 234 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,659,0620CA40.5% 161.2 / 64.3 121A37S (GCG→TCG) gadXacid resistance transcriptional activator GadX
*NZ_CP0092734,091,4350TG40.4% 171.1 / 22.3 109intergenic (+77/‑183)sodA/kdgTsuperoxide dismutase [Mn]/2‑keto‑3‑deoxygluconate transporter
*NZ_CP0092734,277,6380GA38.2% 111.0 / 33.0 76intergenic (‑339/‑54)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NZ_CP0092732,380,3410GA37.3% 92.2 / 44.2 68intergenic (+31/‑72)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NZ_CP0092731,817,6940CT37.3% 91.5 / 26.9 59intergenic (+152/‑78)nadE/choammonia‑dependent NAD(+) synthetase/excinuclease Cho
*NZ_CP0092734,036,3900AG36.7% 102.8 / 10.4 60N206D (AAT→GAT) srkAstress response kinase SrkA
*NZ_CP0092734,277,6470TA36.0% 118.2 / 40.5 75intergenic (‑348/‑45)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NZ_CP0092732,089,5710AG35.8% 156.7 / 25.3 96I83V (ATT→GTT) hisFimidazole glycerol phosphate synthase subunit HisF
*NZ_CP0092733,659,0170CA34.4% 268.5 / 41.4 121V52L (GTA→TTA) gadXacid resistance transcriptional activator GadX
*NZ_CP0092732,897,0740GA33.9% 177.6 / 70.6 109intergenic (+341/+1032)ygcE/queEFGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NZ_CP0092731,817,6850CG33.3% 113.2 / 19.1 60intergenic (+143/‑87)nadE/choammonia‑dependent NAD(+) synthetase/excinuclease Cho
*NZ_CP0092732,503,3590AC33.3% 185.0 / 13.1 90D84A (GAC→GCC) yfeOion channel protein
*NZ_CP0092732,897,0510CA33.0% 218.2 / 57.2 106intergenic (+318/+1055)ygcE/queEFGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NZ_CP0092732,221,7180GC32.7% 77.5 / 13.4 53pseudogene (520/1436 nt)mdtQmultidrug resistance outer membrane protein MdtQ
*NZ_CP0092732,428,3610TG32.4% 160.8 / 11.3 75Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase TruA
*NZ_CP0092733,126,6610TG32.2% 190.8 / 10.9 88H219P (CAC→CCC) yghSdTMP kinase
*NZ_CP0092733,199,8050CA32.1% 103.2 / 20.5 83intergenic (‑190/‑17)ttdR/ttdADNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
*NZ_CP0092733,941,2100CT32.1% 154.0 / 36.1 81E40K (GAA→AAA) hdfRHTH‑type transcriptional regulator HdfR
*NZ_CP0092733,977,4500TC32.1% 209.8 / 19.9 107F378S (TTC→TCC) aslBanaerobic sulfatase maturase
*NZ_CP0092732,428,3420AG31.9% 126.2 / 16.9 74L258L (CTT→CTCtruAtRNA pseudouridine(38‑40) synthase TruA

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 294082647 (0.440)3 (0.040) 3/328 14.4 6.9% intergenic (+4/‑30) BW25113_RS14635/BW25113_RS14640 tRNA‑Met/tRNA‑Met
?NZ_CP009273 2941047 = 50 (0.700)intergenic (+5/+69) BW25113_RS14645/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase AmiC
* ? NZ_CP009273 4532499 =117 (1.080)7 (0.070) 7/476 15.4 5.6% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 123 (1.180)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 = 4532507118 (1.090)6 (0.060) 6/476 16.1 4.8% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 123 (1.180)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 270430 =NA (NA)3 (0.030) 3/488 18.8 NA coding (363/981 nt) BW25113_RS01325 IS5‑like element IS5 family transposase
?NZ_CP009273 270448 = NA (NA)coding (345/981 nt) BW25113_RS01325 IS5‑like element IS5 family transposase
* ? NZ_CP009273 = 4059916103 (0.950)3 (0.030) 3/492 18.9 2.9% coding (639/1386 nt) yihP MFS transporter
?NZ_CP009273 = 4059924 100 (0.930)coding (631/1386 nt) yihP MFS transporter
* ? NZ_CP009273 = 9106673 (0.680)3 (0.030) 3/492 18.9 3.9% coding (1414/1488 nt) murE UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
?NZ_CP009273 = 91082 75 (0.700)coding (1430/1488 nt) murE UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase