breseq  version 0.33.1  revision 8505477f25b3 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 228 shown, sorted by frequency from high to low)
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,515,909	0	A	C	54.9%	59.5 / 106.1	71	V260G (GTG→GGG)	ypfI	predicted hydrolase
*	REL606	720,464	0	C	G	50.0%	3.0 / 23.9	20	A22G (GCC→GGC)	phrB	deoxyribodipyrimidine photolyase, FAD‑binding
*	REL606	1,854,401	0	A	C	45.9%	74.7 / 53.3	85	K88N (AAA→AAC)	yeaO	hypothetical protein
*	REL606	433	0	T	G	44.3%	71.5 / 50.5	70	V33G (GTG→GGG)	thrA	bifunctional aspartokinase I/homeserine dehydrogenase I
*	REL606	2,437,406	0	G	T	40.7%	35.0 / 17.7	27	D435E (GAC→GAA)	ypdD	fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component
*	REL606	2,376,348	0	T	G	39.5%	211.6 / 22.8	131	T63P (ACC→CCC)	folC	bifunctional folylpolyglutamate synthase/ dihydrofolate synthase
*	REL606	895,856	0	G	C	39.5%	51.9 / 31.1	38	A26P (GCC→CCC)	ECB_00836	putative Regulatory protein
*	REL606	1,906,430	0	A	C	39.1%	251.7 / 52.7	133	V329G (GTG→GGG)	ptrB	protease II
*	REL606	4,111,429	0	T	G	38.8%	105.1 / 18.9	67	G496G (GGT→GGG)	metL	bifunctional aspartate kinase II/homoserine dehydrogenase II
*	REL606	4,180,249	0	T	G	38.2%	198.0 / 24.3	111	G24G (GGT→GGG)	yjaH	hypothetical protein
*	REL606	3,378,885	0	T	G	36.6%	463.2 / 66.0	224	T129P (ACC→CCC)	rplB	50S ribosomal protein L2
*	REL606	157,994	0	G	T	36.4%	254.0 / 163.9	140	Q93K (CAA→AAA)	htrE	predicted outer membrane usher protein
*	REL606	1,485,450	0	A	G	36.1%	348.0 / 120.0	194	G193G (GGA→GGG)	ydcV	predicted spermidine/putrescine transporter subunit
*	REL606	2,523,349	0	A	C	35.9%	226.6 / 44.1	117	H235P (CAC→CCC)	hyfB	NADH dehydrogenase subunit N
*	REL606	1,118,209	0	G	T	35.3%	38.5 / 25.9	34	intergenic (‑444/‑311)	csgD/csgB	DNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex
*	REL606	3,783,565	0	A	C	34.6%	178.1 / 45.0	101	T744P (ACA→CCA)	ECB_03521	conserved hypothetical protein
*	REL606	3,217,434	0	G	C	34.1%	41.6 / 23.6	44	A76P (GCC→CCC)	agaS	tagatose‑6‑phosphate ketose/aldose isomerase
*	REL606	2,685,638	0	T	G	33.6%	235.4 / 38.1	110	V326G (GTG→GGG)	ygaF	predicted enzyme
*	REL606	851,606	0	G	A	33.6%	154.2 / 80.1	113	S3F (TCT→TTT)	ybiU	hypothetical protein
*	REL606	4,595,070	0	A	G	33.6%	278.0 / 80.6	149	G11G (GGA→GGG)	yjjZ	hypothetical protein

Marginal new junction evidence (sorted from low to high skew)
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	1532002 =	227 (1.050)	5 (0.030)	3/130	19.4	2.3%	coding (954/987 nt)	yddP	D‑ala‑D‑ala transporter subunit
	?	REL606	= 3014081	228 (1.220)					coding (662/1137 nt)	ECB_02815	Capsule polysaccharide export inner‑membrane protein kpsE
*	?	REL606	984122 =	261 (1.210)	3 (0.020)	3/130	19.4	1.3%	coding (410/1749 nt)	msbA	fused lipid transporter subunits of ABC superfamily: membrane component/ATP‑binding component
	?	REL606	= 984118	219 (1.180)					coding (406/1749 nt)	msbA	fused lipid transporter subunits of ABC superfamily: membrane component/ATP‑binding component
*	?	REL606	= 2525354	173 (0.800)	3 (0.020)	3/132	19.5	1.6%	pseudogene (453/720 nt)	hyfC	hydrogenase 4 membrane subunit; b2483_1
	?	REL606	= 2718592	210 (1.110)					coding (37/1062 nt)	recA	recombinase A