Predicted mutation
evidence seq id position mutation annotation gene description
JC JC REL606 1,886,669 IS1 (–) +9 bp intergenic (‑499/+162) yobF ← / ← yebO hypothetical protein/hypothetical protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 1886669 =9 (0.210)91 (1.870) 64/150 0.0 89.9% intergenic (‑499/+170) yobF/yebO hypothetical protein/hypothetical protein
?REL606 = 2035093 NA (NA)noncoding (1/768 nt) IS1 repeat region
* ? REL606 = 18866779 (0.210)60 (1.240) 45/150 0.1 85.4% intergenic (‑507/+162) yobF/yebO hypothetical protein/hypothetical protein
?REL606 = 1974039 NA (NA)noncoding (768/768 nt) IS1 repeat region

AGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAAATATCCTTGTGATAATATTTATTATAAAGTCTTGATATGATATATAATTTGCACGATAAAAATG  >  REL606/2035024‑2035160
                                                                     |                                                                   
aGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAAata                                                               >  1:1413654/1‑76 (MQ=34)
              cTGGAGCGCCTCAAAAACACCATCATACACTAAATCAGTAAGTTGTCAGCATCACCCAAATATCCTTGTGataata                                                 <  1:1067239/76‑1 (MQ=255)
                                                             agcaTCACCCAAATATCCTTGTGATAATATTTATTATAAAGTCTTGATATGATATATAATTTGCACGATAAAAATg  >  1:316024/1‑76 (MQ=255)
                                                                     |                                                                   
AGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAAATATCCTTGTGATAATATTTATTATAAAGTCTTGATATGATATATAATTTGCACGATAAAAATG  >  REL606/2035024‑2035160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.