breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 2,296,329 | 0 | A | C | 46.5% | 74.4 / 55.3 | 71 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
* | REL606 | 3,492,082 | 0 | T | G | 38.1% | 94.7 / 32.5 | 63 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
* | REL606 | 3,983,700 | 0 | T | G | 34.5% | 34.6 / 11.4 | 29 | T397P (ACC→CCC) | ECB_03713 | conserved hypothetical protein |
* | REL606 | 4,141,009 | 0 | G | A | 33.3% | 102.2 / 25.0 | 51 | E28K (GAA→AAA) | yijC | DNA‑binding transcriptional repressor |
* | REL606 | 1,875,483 | 0 | G | C | 32.7% | 203.8 / 108.2 | 113 | intergenic (+156/‑28) | yeaB/sdaA | predicted NUDIX hydrolase/L‑serine deaminase I |
* | REL606 | 3,998,787 | 0 | A | C | 32.4% | 125.7 / 13.3 | 68 | T31P (ACC→CCC) | ubiB | putative ubiquinone biosynthesis protein UbiB |
* | REL606 | 4,195,874 | 0 | A | C | 32.1% | 94.7 / 25.1 | 53 | T318P (ACC→CCC) | aceB | malate synthase |
* | REL606 | 2,631,772 | 0 | A | C | 30.9% | 83.2 / 13.0 | 42 | intergenic (‑267/‑141) | rpoE/nadB | RNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase |
* | REL606 | 273,581 | 0 | T | G | 30.0% | 126.4 / 12.5 | 60 | G136G (GGT→GGG) | yagU | conserved inner membrane protein |
* | REL606 | 275,418 | 0 | A | C | 28.6% | 67.7 / 11.1 | 36 | F528V (TTT→GTT) | yagW | predicted receptor |
* | REL606 | 1,004,106 | 0 | A | C | 27.8% | 166.7 / 22.0 | 72 | intergenic (‑32/+570) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
* | REL606 | 1,882,526 | 0 | T | G | 27.7% | 127.5 / 11.1 | 65 | I21S (ATT→AGT) | manZ | mannose‑specific enzyme IID component of PTS |
* | REL606 | 4,393,061 | 0 | T | G | 26.8% | 115.9 / 20.3 | 57 | V211G (GTG→GGG) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 4,574,252 | 0 | T | G | 25.5% | 100.3 / 16.6 | 47 | S5S (TCA→TCC) | hpaX | hypothetical 4‑hydroxyphenylacetate permease |
* | REL606 | 2,829,417 | 0 | T | C | 25.5% | 108.0 / 11.5 | 51 | E106G (GAG→GGG) | ECB_02652 | conserved hypothetical protein |
* | REL606 | 154,497 | 0 | T | G | 25.4% | 139.3 / 20.7 | 63 | A191A (GCA→GCC) | yadL | predicted fimbrial‑like adhesin protein |
* | REL606 | 1,484,105 | 0 | A | G | 22.4% | 117.1 / 12.0 | 49 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | REL606 | 1,747,903 | 0 | G | T | 21.2% | 302.3 / 51.8 | 99 | A8S (GCA→TCA) | ydiL | hypothetical protein |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 4201708 | 82 (1.280) | 3 (0.050) | 3/136 | 7.9 | 3.9% | intergenic (‑290/+27) | arpA/iclR | regulator of acetyl CoA synthetase; ankyrin‑like regulatory protein; b4017_1/DNA‑binding transcriptional repressor |
? | REL606 | = 4201716 | 74 (1.280) | intergenic (‑298/+19) | arpA/iclR | regulator of acetyl CoA synthetase; ankyrin‑like regulatory protein; b4017_1/DNA‑binding transcriptional repressor |