breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 296,977 G→A E191K (GAA→AAA)  ykgG → predicted transporter
RA 475,286 A→C L7R (CTT→CGT)  ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
RA 650,345 A→C F153V (TTT→GTT)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
JC 2,298,075 Δ9 bp coding (1000‑1008/1359 nt) glpT ← sn‑glycerol‑3‑phosphate transporter
MC JC 3,014,068 (AAC)3→2 coding (649‑651/1137 nt) ECB_02815 → Capsule polysaccharide export inner‑membrane protein kpsE
MC JC 3,228,513 Δ46 bp coding (12‑57/861 nt) yraL ← predicted methyltransferase
JC JC 3,570,726 IS150 (–) +3 bp intergenic (‑317/‑72) yhiO ← / → uspA universal stress protein UspB/universal stress global response regulator
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
RA 3,876,073 T→C D89G (GAT→GGT)  atpC ← F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
JC 4,108,659 (TATTT)1→2 intergenic (‑157/‑120) metJ ← / → metB transcriptional repressor protein MetJ/cystathionine gamma‑synthase
RA 4,166,065 C→T P369S (CCG→TCG)  rpoC → DNA‑directed RNA polymerase subunit beta'
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 589608–590469 593379 2911–3772 23 [20] [20] 21 [insK‑1]–[fepA] [insK‑1],[insB‑7],insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
* * ÷ REL606 593571 619840–619116 25546–26270 23 [20] [20] 22 [fepA]–insA‑8 [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8