breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 1,875,483 | 0 | G | C | 46.7% | 47.1 / 142.7 | 92 | intergenic (+156/‑28) | yeaB/sdaA | predicted NUDIX hydrolase/L‑serine deaminase I |
* | REL606 | 2,296,329 | 0 | A | C | 45.6% | 56.2 / 41.6 | 57 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
* | REL606 | 2,631,772 | 0 | A | C | 41.7% | 59.1 / 27.6 | 48 | intergenic (‑267/‑141) | rpoE/nadB | RNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase |
* | REL606 | 3,492,082 | 0 | T | G | 40.5% | 51.0 / 14.5 | 37 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
* | REL606 | 4,574,252 | 0 | T | G | 38.5% | 28.1 / 15.7 | 26 | S5S (TCA→TCC) | hpaX | hypothetical 4‑hydroxyphenylacetate permease |
* | REL606 | 3,691,844 | 0 | T | G | 36.8% | 51.2 / 17.8 | 38 | N226T (AAC→ACC) | yiaY | predicted Fe‑containing alcohol dehydrogenase |
* | REL606 | 273,581 | 0 | T | G | 36.4% | 107.7 / 23.2 | 66 | G136G (GGT→GGG) | yagU | conserved inner membrane protein |
* | REL606 | 1,747,903 | 0 | G | T | 36.0% | 179.7 / 101.1 | 100 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 154,497 | 0 | T | G | 31.4% | 102.6 / 14.8 | 51 | A191A (GCA→GCC) | yadL | predicted fimbrial‑like adhesin protein |
* | REL606 | 4,195,874 | 0 | A | C | 29.5% | 87.6 / 13.0 | 44 | T318P (ACC→CCC) | aceB | malate synthase |
* | REL606 | 716,832 | 0 | T | G | 28.3% | 127.8 / 15.0 | 60 | A31A (GCA→GCC) | ECB_00663 | hypothetical protein |
* | REL606 | 4,393,061 | 0 | T | G | 27.5% | 82.1 / 10.1 | 40 | V211G (GTG→GGG) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 1,484,105 | 0 | A | G | 26.8% | 86.1 / 12.7 | 41 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | REL606 | 4,169,232 | 0 | A | T | 23.1% | 136.5 / 11.2 | 53 | intergenic (+48/‑165) | rpoC/htrC | DNA‑directed RNA polymerase subunit beta'/heat shock protein |
* | REL606 | 2,417,495 | 0 | T | A | 22.2% | 153.1 / 15.1 | 54 | L24F (TTA→TTT) | emrK | EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component |
* | REL606 | 4,396,513 | 0 | C | G | 22.2% | 93.1 / 11.2 | 36 | S32S (TCG→TCC) | yjfQ | DNA‑binding transcriptional dual regulator |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 3171156 | 64 (1.090) | 4 (0.080) | 3/130 | 6.6 | 6.6% | intergenic (+42/‑241) | ygjR/ygjT | predicted NAD(P)‑binding dehydrogenase/predicted inner membrane protein, part of terminus |
? | REL606 | = 3171165 | 58 (1.140) | intergenic (+51/‑232) | ygjR/ygjT | predicted NAD(P)‑binding dehydrogenase/predicted inner membrane protein, part of terminus | |||||
* | ? | REL606 | 562246 = | 67 (1.140) | 3 (0.050) | 3/142 | 6.9 | 4.6% | coding (14/762 nt) | envY | DNA‑binding transcriptional activator of porin biosynthesis |
? | REL606 | 562484 = | 62 (1.120) | coding (849/891 nt) | ybcH | hypothetical protein |