breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 990,162 T→G E111A (GAA→GCA)  ycbC ← conserved inner membrane protein
JC JC 1,305,487 IS150 (–) +3 bp coding (751‑753/1461 nt) cls ← cardiolipin synthetase
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 1,893,840 A→C L91R (CTG→CGG)  proQ ← putative solute/DNA competence effector
MC JC 2,103,915 (CCAG)7→6 coding (182‑185/216 nt) ECB_01992 → hypothetical protein
RA 2,105,116 (A)8→7 intergenic (+77/+135) ECB_01993 → / ← ECB_01994 hypothetical protein/hypothetical protein
RA 2,114,852 (T)8→7 coding (187/366 nt) ECB_02002 ← hypothetical protein
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
JC JC 3,270,445 IS150 (+) +2 bp :: +CGT intergenic (+23/+24) nlp → / ← murA DNA‑binding transcriptional activator of maltose metabolism/UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
JC 3,571,131 +GTACA coding (332/435 nt) uspA → universal stress global response regulator
RA 3,612,971 T→C intergenic (‑32/+151) dctA ← / ← yhjK C4‑dicarboxylate transport protein/predicted diguanylate cyclase
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,928,783 G→T Q189H (CAG→CAT rho → transcription termination factor Rho
RA 4,100,824 A→T I136N (ATC→AAC)  hslU ← ATP‑dependent protease ATP‑binding subunit
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 3571132 =2 (0.040)195 (3.420) 97/146 0.0 99.0% coding (333/435 nt) uspA universal stress global response regulator
?REL606 = 3652533 NA (NA)noncoding (1443/1443 nt) IS150 repeat region