breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 4,393,061 | 0 | T | G | 43.8% | 38.6 / 16.0 | 33 | V211G (GTG→GGG) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 2,296,329 | 0 | A | C | 39.5% | 67.0 / 19.9 | 43 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
* | REL606 | 3,691,844 | 0 | T | G | 37.7% | 95.4 / 17.5 | 53 | N226T (AAC→ACC) | yiaY | predicted Fe‑containing alcohol dehydrogenase |
* | REL606 | 3,983,700 | 0 | T | G | 34.2% | 71.8 / 13.7 | 41 | T397P (ACC→CCC) | ECB_03713 | conserved hypothetical protein |
* | REL606 | 3,492,082 | 0 | T | G | 34.1% | 89.0 / 11.0 | 44 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
* | REL606 | 3,733,719 | 0 | C | G | 33.3% | 59.8 / 10.9 | 30 | G63G (GGC→GGG) | gumD | ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding |
* | REL606 | 1,176,010 | 0 | T | G | 32.6% | 102.1 / 11.0 | 49 | A37A (GCA→GCC) | fhuE | ferric‑rhodotorulic acid outer membrane transporter |
* | REL606 | 1,484,105 | 0 | A | G | 30.6% | 59.9 / 16.4 | 36 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | REL606 | 1,882,526 | 0 | T | G | 23.7% | 111.6 / 10.3 | 59 | I21S (ATT→AGT) | manZ | mannose‑specific enzyme IID component of PTS |
* | REL606 | 1,875,483 | 0 | G | C | 23.7% | 240.9 / 67.6 | 93 | intergenic (+156/‑28) | yeaB/sdaA | predicted NUDIX hydrolase/L‑serine deaminase I |
* | REL606 | 1,747,903 | 0 | G | T | 20.4% | 129.8 / 22.1 | 44 | A8S (GCA→TCA) | ydiL | hypothetical protein |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 2041277 = | 13 (0.260) | 4 (0.090) | 3/134 | 5.7 | 23.7% | coding (107/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | 2041470 = | 14 (0.310) | coding (1351/1428 nt) | wzx | O antigen translocase (Wzx) | |||||
* | ? | REL606 | 3022554 = | 41 (0.820) | 3 (0.070) | 3/136 | 5.8 | 7.4% | coding (131/1140 nt) | ECB_02821 | hypothetical protein |
? | REL606 | 3022717 = | 37 (0.810) | intergenic (‑33/+541) | ECB_02821/ECB_02822 | hypothetical protein/bifunctional Ribulose 5‑phosphate reductase/CDP‑ribitol pyrophosphorylase |