breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,393,0610TG43.8% 38.6 / 16.0 33V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL6062,296,3290AC39.5% 67.0 / 19.9 43intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL6063,691,8440TG37.7% 95.4 / 17.5 53N226T (AAC→ACC) yiaYpredicted Fe‑containing alcohol dehydrogenase
*REL6063,983,7000TG34.2% 71.8 / 13.7 41T397P (ACC→CCC) ECB_03713conserved hypothetical protein
*REL6063,492,0820TG34.1% 89.0 / 11.0 44V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6063,733,7190CG33.3% 59.8 / 10.9 30G63G (GGC→GGGgumDADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding
*REL6061,176,0100TG32.6% 102.1 / 11.0 49A37A (GCA→GCCfhuEferric‑rhodotorulic acid outer membrane transporter
*REL6061,484,1050AG30.6% 59.9 / 16.4 36G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*REL6061,882,5260TG23.7% 111.6 / 10.3 59I21S (ATT→AGT) manZmannose‑specific enzyme IID component of PTS
*REL6061,875,4830GC23.7% 240.9 / 67.6 93intergenic (+156/‑28)yeaB/sdaApredicted NUDIX hydrolase/L‑serine deaminase I
*REL6061,747,9030GT20.4% 129.8 / 22.1 44A8S (GCA→TCA) ydiLhypothetical protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2041277 =13 (0.260)4 (0.090) 3/134 5.7 23.7% coding (107/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2041470 = 14 (0.310)coding (1351/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 3022554 =41 (0.820)3 (0.070) 3/136 5.8 7.4% coding (131/1140 nt) ECB_02821 hypothetical protein
?REL606 3022717 = 37 (0.810)intergenic (‑33/+541) ECB_02821/ECB_02822 hypothetical protein/bifunctional Ribulose 5‑phosphate reductase/CDP‑ribitol pyrophosphorylase