breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,209,4200CG56.0% ‑0.7 / 22.4 26pseudogene (200/501 nt)stfEpseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
*NC_0009131,527,6020GC40.9% 26.8 / 16.6 22intergenic (+450/‑300)yncH/rhsEIPR020099 family protein/pseudogene, Rhs family
*NC_00091386,3650GC40.0% 13.8 / 11.1 20A246P (GCG→CCG) ilvIacetolactate synthase 3 large subunit
*NC_0009132,253,0230CG38.1% 27.4 / 12.6 21A375P (GCG→CCG) nupXnucleoside permease
*NC_0009132,879,5360CG37.0% 43.6 / 12.8 28G79G (GGG→GGCygbTmultifunctional endonuclease Cas1, CRISPR adaptation protein; DNA repair enzyme
*NC_0009131,135,9750GC36.0% 28.8 / 18.8 25A138P (GCC→CCC) flgHflagellar protein of basal‑body outer‑membrane L ring
*NC_0009131,041,3860GC35.7% 41.5 / 11.6 28G257A (GGA→GCA) appAphosphoanhydride phosphorylase
*NC_0009131,984,1880CG33.3% 37.9 / 13.0 24G294G (GGG→GGCaraGL‑arabinose ABC transporter ATPase
*NC_0009134,338,1300GC33.3% 12.9 / 10.3 12intergenic (‑201/+124)adiY/adiAadi system transcriptional activator/arginine decarboxylase
*NC_0009131,081,3530GC32.1% 47.9 / 10.6 28intergenic (+540/‑3)putP/efeUproline:sodium symporter/ferrous iron permease (pseudogene)
*NC_0009133,593,3090CG30.8% 48.7 / 10.9 26G43G (GGG→GGClivFbranched‑chain amino acid ABC transporter ATPase
*NC_000913516,8930GC30.3% 53.3 / 16.1 34G104A (GGA→GCA) fetBiron export ABC transporter permease; peroxide resistance protein
*NC_0009134,296,0600CT30.0% 74.0 / 40.9 51intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_0009134,164,1320GC29.4% 38.3 / 15.4 35G165A (GGA→GCA) btuBvitamin B12/cobalamin outer membrane transporter
*NC_000913391,7010GC28.9% 81.1 / 11.2 39pseudogene (1451/1458 nt)yaiTpseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
*NC_0009131,618,0750GC28.1% 60.8 / 10.3 32A350P (GCG→CCG) ydeAarabinose efflux transporter, arabinose‑inducible
*NC_00091334,5080GC27.3% 39.6 / 10.5 22G70A (GGA→GCA) caiFcai operon transcriptional activator

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =28 (0.990)4 (0.190) 4/100 1.6 14.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 26 (1.220)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1828864 =15 (0.530)3 (0.120) 3/114 2.2 19.4% coding (871/1479 nt) astD succinylglutamic semialdehyde dehydrogenase
?NC_000913 1828903 = 12 (0.490)coding (832/1479 nt) astD succinylglutamic semialdehyde dehydrogenase
* ? NC_000913 523984 =23 (0.810)3 (0.120) 3/116 2.3 12.2% coding (724/4281 nt) rhsD Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 524076 = 23 (0.930)coding (816/4281 nt) rhsD Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor