breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 148,861 G→C R147G (CGC→GGC)  yadM ← putative fimbrial‑like adhesin protein
RA 3,178,277 G→T E27* (GAA→TAA)  yqiC → BMFP family putative fusogenic protein
RA 3,412,459 +A pseudogene (59/66 nt) yhdW → pseudogene, amino‑acid transporter homology;putative transport; Not classified; putative periplasmic binding transport protein; periplasmic‑binding component of ABC superfamily
RA 4,049,665 A→C I12S (ATC→AGC)  glnL ← sensory histidine kinase in two‑component regulatory system with GlnG
RA 4,546,084 G→T H18Q (CAC→CAA yjiC ← uncharacterized protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 1836566 1837995 1430 24 [21] [21] 22 gdhA gdhA
* * ÷ CP009273 3347945 3352558 4614 22 [21] [21] 22 gltB–[gltD] gltB, [gltD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =NA (NA)73 (1.070) 60/294 0.1 NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =81 (1.190)23 (0.380) 22/262 2.1 23.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 74 (1.220)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326181 (1.190)37 (0.610) 31/262 1.1 33.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 74 (1.220)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 1390301 =NA (NA)102 (1.500) 74/294 0.0 100% intergenic (‑122/‑32) ynaI/insH1 mechanosensitive channel protein, very small conductance/IS5 transposase and trans‑activator
?CP009273 = 1539172 0 (0.000)pseudogene (800/951 nt) yddK pseudogene, leucine‑rich protein; putative glycoportein
* ? CP009273 = 1391496NA (NA)46 (0.690) 38/290 0.9 97.9% intergenic (+147/‑126) insH1/ynaJ IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein
?CP009273 1539170 = 1 (0.010)pseudogene (802/951 nt) yddK pseudogene, leucine‑rich protein; putative glycoportein
* ? CP009273 1973060 =2 (0.030)58 (0.850) 46/294 0.5 96.7% intergenic (‑606/‑174) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 = 19730684 (0.060)65 (0.960) 55/294 0.2 94.2% intergenic (‑614/‑166) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA
* ? CP009273 = 3180785NA (NA)62 (0.910) 51/294 0.3 96.9% pseudogene (6/2445 nt) yqiG pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein
?CP009273 3710988 = 2 (0.030)coding (319/975 nt) ghrB glyoxylate/hydroxypyruvate reductase B
* ? CP009273 = 37109925 (0.070)78 (1.150) 62/294 0.1 94.0% coding (323/975 nt) ghrB glyoxylate/hydroxypyruvate reductase B
?CP009273 4487998 = NA (NA)intergenic (+241/‑91) intB/insC1 pseudogene, integrase homology;IS, phage, Tn; Phage‑related functions and prophages; KpLE2 phage‑like element; P4‑like integrase/IS2 repressor TnpA