breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 572,216 C→T 30.8% pseudogene (66/1040 nt) nmpC ← DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage
RA 764,350 T→C 44.4% L306P (CTG→CCG)  mngB → alpha‑mannosidase
RA 882,839 T→C 21.2% intergenic (‑44/‑40) ybjJ ← / → rcdA putative transporter/transcriptional regulator of csgD and ybiJI; autoregulator
RA 2,051,591 G→T 69.1% intergenic (‑8/+93) asnW ← / ← yeeO tRNA‑Asn/putative multdrug exporter, MATE family
JC JC 2,400,257 IS5 (+) +4 bp 87.2% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
RA 2,420,353 A→C 75.1% intergenic (‑86/+135) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
JC JC 3,178,254 IS5 (+) +4 bp 54.3% coding (56‑59/291 nt) yqiC → BMFP family putative fusogenic protein
RA 3,178,277 G→T 42.1% E27* (GAA→TAA)  yqiC → BMFP family putative fusogenic protein
RA 4,050,838 A→T 59.8% V186E (GTA→GAA)  glnA ← glutamine synthetase
RA 4,546,084 G→T 53.6% H18Q (CAC→CAA yjiC ← uncharacterized protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 881400 881433 34 2 [1] [0] 2 ybjI 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase; pyrimidine phosphatase; riboflavin synthesis
* * ÷ CP009273 2084775 2084822 48 2 [1] [0] 2 hisD bifunctional histidinal dehydrogenase/ histidinol dehydrogenase
* * ÷ CP009273 2084973 2085072 100 2 [0] [1] 2 hisD bifunctional histidinal dehydrogenase/ histidinol dehydrogenase
* * ÷ CP009273 4051946 4051982 37 2 [1] [1] 2 typA GTP‑binding protein
* * ÷ CP009273 4436804 4436812 9 2 [1] [1] 2 tamB translocation and assembly module for autotransporter export, inner membrane subunit

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 287112NA (NA)5 (0.350) 5/274 NT 55.6% noncoding (1/768 nt) IS1 repeat region
?CP009273 = 1973068 4 (0.280)intergenic (‑614/‑166) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 360104 =NA (NA)21 (1.460) 17/274 NT 55.3% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 17 (1.180)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 376716 =NA (NA)6 (0.420) 5/274 NT 60.0% intergenic (‑1/‑91) yaiX/insC1 pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase/IS2 repressor TnpA
?CP009273 559695 = 4 (0.280)coding (242/633 nt) sfmZ response regulator family protein
* ? CP009273 = 378045NA (NA)10 (0.700) 10/272 NT 66.8% intergenic (+10/+2) insD1/yaiX IS2 transposase TnpB/pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase
?CP009273 = 559700 5 (0.350)coding (237/633 nt) sfmZ response regulator family protein
* ? CP009273 1203246 =2 (0.140)6 (0.470) 6/242 NT 60.7% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 6 (0.470)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 12032612 (0.140)7 (0.550) 5/242 NT 64.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 6 (0.470)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 1973060 =5 (0.350)4 (0.280) 4/274 NT 44.4% intergenic (‑606/‑174) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 2399187 =11 (0.760)5 (0.350) 5/274 NT 31.2% coding (934/939 nt) lrhA transcriptional repressor of flagellar, motility and chemotaxis genes
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 = 245554825 (1.730)6 (0.420) 6/272 NT 19.5% coding (764/1341 nt) fadL long‑chain fatty acid outer membrane transporter
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA