breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 755,832 Δ2 bp coding (273‑274/348 nt) sdhD → succinate dehydrogenase, membrane subunit, binds cytochrome b556
RA 932,304 C→T intergenic (‑254/‑291) trxB ← / → lrp thioredoxin reductase, FAD/NAD(P)‑binding/leucine‑responsive global transcriptional regulator
RA 1,115,627 C→T intergenic (‑137/+35) mdtG ← / ← lpxL putative drug efflux system protein/lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase
RA 1,756,553 A→T K286* (AAA→TAA)  pykF → pyruvate kinase I
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
JC JC 2,270,300 IS5 (+) +4 bp coding (322‑325/567 nt) mepS → murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase
RA 2,810,760 T→C intergenic (+81/‑10) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
RA 2,966,951 A→G V495A (GTC→GCC)  ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
RA 3,338,928 C→A L107F (TTG→TTT mlaE ← ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
JC JC 3,410,998 IS2 (+) +5 bp coding (719‑723/966 nt) dusB → tRNA‑dihydrouridine synthase B
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
RA 3,966,598 G→A G61E (GGA→GAA)  rho → transcription termination factor
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423709–3424234 3424600–3424238 5–892 76 [74] [73] 76 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)201 (1.490) 112/190 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein