breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 65 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,213,4580CA54.4% 36.5 / inf 388R396L (CGC→CTC) yehUinner membrane putative sensory kinase in two‑component system with YehT
*NC_0009132,312,5780GT52.1% 22.3 / inf 410R58S (CGT→AGT) ompCouter membrane porin protein C
*NC_0009131,189,8080GT52.1% 52.9 / inf 341R214S (CGC→AGC) phoPresponse regulator in two‑component regulatory system with PhoQ
*NC_0009134,186,4250CT51.5% 44.6 / inf 424P359L (CCA→CTA) rpoCRNA polymerase, beta prime subunit
*NC_0009131,910,6540GT51.0% 52.9 / inf 298A127S (GCA→TCA) yebQputative transporter
*NC_0009131,940,4620GT50.5% 122.5 / inf 377P392T (CCG→ACG) mepMmurein DD‑endopeptidase, space‑maker hydrolase, septation protein
*NC_0009133,310,4610CA50.5% 141.3 / inf 382E236D (GAG→GATpnppolynucleotide phosphorylase/polyadenylase
*NC_0009132,396,7400CA50.1% 38.7 / inf 386G235C (GGT→TGT) nuoHNADH:ubiquinone oxidoreductase, membrane subunit H
*NC_0009132,456,1630CA49.8% 142.3 / inf 515intergenic (‑517/+164)yfcV/sixAputative fimbrial‑like adhesin protein/phosphohistidine phosphatase
*NC_0009132,063,0690CA49.0% 118.0 / inf 410P85P (CCG→CCTldtAL,D‑transpeptidase linking Lpp to murein
*NC_000913736,6860GT48.5% 137.6 / inf 414intergenic (+2/‑139)ybfQ/ybfLpseudogene, H repeat‑associated protein/pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
*NC_0009134,183,2250GC48.5% 74.4 / inf 455V661L (GTA→CTA) rpoBRNA polymerase, beta subunit
*NC_0009131,151,7480TC48.4% 47.2 / inf 381M45T (ATG→ACG) acpPacyl carrier protein (ACP)
*NC_0009132,640,1170GT48.2% 152.0 / inf 394R153R (CGC→CGAhisShistidyl tRNA synthetase
*NC_0009133,642,9020CA48.1% 166.4 / inf 400A77A (GCG→GCTrsmJ16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
*NC_0009134,105,4320CA48.1% 188.6 / inf 421R80L (CGC→CTC) cpxRresponse regulator in two‑component regulatory system with CpxA
*NC_0009131,961,2400GT48.1% 166.4 / inf 339L393L (CTG→CTTargSarginyl‑tRNA synthetase
*NC_0009133,653,3670CA48.0% 167.8 / inf 519pseudogene (137/483 nt)yhiSpseudogene
*NC_0009132,037,1010CA48.0% 230.4 / inf 467R351L (CGA→CTA) yedVputative sensory kinase in two‑component regulatory system with YedW
*NC_000913261,0480GT47.4% 205.6 / inf 392A221S (GCT→TCT) proBgamma‑glutamate kinase

Marginal new junction evidence (lowest skew 10 of 52 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 687851 =NA (NA)218 (0.530) 139/300 3.9 50.1% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 889537 217 (0.530)coding (283/1686 nt) ybjL putative transporter
* ? NC_000913 2413601 =238 (0.580)222 (0.540) 136/300 4.2 48.3% coding (132/1203 nt) ackA acetate kinase A and propionate kinase 2
?NC_000913 3583428 = NA (NA)noncoding (1/768 nt) IS1 repeat region
* ? NC_000913 = 258675NA (NA)196 (0.480) 134/300 4.4 48.9% noncoding (1/768 nt) IS1 repeat region
?NC_000913 1307993 = 205 (0.500)coding (653/1461 nt) clsA cardiolipin synthase 1
* ? NC_000913 = 1308001210 (0.510)180 (0.440) 126/300 5.4 46.2% coding (645/1461 nt) clsA cardiolipin synthase 1
?NC_000913 1978503 = NA (NA)noncoding (768/768 nt) IS1 repeat region
* ? NC_000913 = 1050545NA (NA)180 (0.440) 125/300 5.5 46.2% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 1308001 210 (0.510)coding (645/1461 nt) clsA cardiolipin synthase 1
* ? NC_000913 = 291401NA (NA)193 (0.470) 119/300 6.4 44.8% noncoding (1/768 nt) IS1 repeat region
?NC_000913 2413601 = 238 (0.580)coding (132/1203 nt) ackA acetate kinase A and propionate kinase 2
* ? NC_000913 = 291401NA (NA)183 (0.440) 116/300 6.8 47.2% noncoding (1/768 nt) IS1 repeat region
?NC_000913 1307993 = 205 (0.500)coding (653/1461 nt) clsA cardiolipin synthase 1
* ? NC_000913 781369 =263 (0.640)144 (0.360)
+GAAG
63/292 17.0 39.9% noncoding (1/76 nt) lysZ tRNA‑Lys
?NC_000913 = 2521006 183 (0.440)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 781577 =301 (0.730)217 (0.540)
+GAAG
62/292 17.3 48.0% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 183 (0.440)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 780554 =511 (1.240)120 (0.300)
+GAAG
62/292 17.3 26.2% noncoding (1/76 nt) lysT tRNA‑Lys
?NC_000913 = 2521006 183 (0.440)noncoding (76/76 nt) valU tRNA‑Val