breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 250,396 A→G 14.8% pseudogene (325/756 nt) lafU → flagellar system protein, promoterless fragment
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
JC JC 622,244 IS5 (–) +4 bp 55.2% intergenic (‑55/‑53) fepD ← / → entS ferric enterobactin ABC transporter membrane subunit FebD/enterobactin exporter EntS
RA 1,009,421 C→T 8.5% H526H (CAC→CAT rlmL → fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,650,461 C→A 39.1% pseudogene (384/921 nt) ydfE → Qin prophage; protein YdfE
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
JC JC 2,629,042 IS5 (+) +4 bp 54.3% intergenic (‑104/‑245) pdeF ← / → yfgG cyclic di‑GMP phosphodiesterase PdeF/protein YfgG
RA 2,708,714 C→A 11.1% S14I (AGC→ATC)  rseB ← anti‑sigma factor stabilizing protein RseB
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 4,105,639 C→A 9.6% R11L (CGA→CTA)  cpxR ← DNA‑binding transcriptional dual regulator CpxR
RA 4,296,060 C→T 54.3% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,510,928 A→T 64.4% L177Q (CTG→CAG)  fecE ← ferric citrate ABC transporter ATP binding subunit
RA 4,514,119 C→T 31.5% D64N (GAT→AAT)  fecB ← ferric citrate ABC transporter periplasmic binding protein
RA 4,515,480 C→T 100% G400S (GGC→AGC)  fecA ← ferric citrate outer membrane transporter
RA 4,554,433 C→A 10.2% intergenic (+72/‑143) uxuB → / → uxuR D‑mannonate oxidoreductase/DNA‑binding transcriptional repressor UxuR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423764–3424234 3424496–3424239 6–733 7 [6] [6] 8 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 62168822 (0.710)5 (0.170) 3/356 NT 17.0% coding (502/1005 nt) fepD ferric enterobactin ABC transporter membrane subunit FebD
?NC_000913 623383 = 28 (0.960)coding (1084/1251 nt) entS enterobactin exporter EntS
* ? NC_000913 1207790 =22 (0.710)19 (0.670) 18/344 NT 49.3% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 19 (0.670)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780522 (0.710)12 (0.420) 11/344 NT 38.0% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 19 (0.670)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 2288919 =NA (NA)5 (0.160) 4/376 NT 12.2% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 3922507 36 (1.160)intergenic (‑67/+550) atpI/rsmG ATP synthase accessory factor/16S rRNA m(7)G527 methyltransferase
* ? NC_000913 = 454269021 (0.680)3 (0.100) 3/358 NT 10.9% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 29 (0.980)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit