breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 258,768 G→A 19.2% pseudogene (93/331 nt) crl → RNA polymerase holoenzyme assembly factor Crl
RA 1,076,773 C→A 8.5% A704S (GCT→TCT)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,955,394 C→T 10.2% A538A (GCG→GCA torZ ← trimethylamine N‑oxide reductase 2
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,712,451 A→G 9.7% I105T (ATT→ACT)  yfiC ← tRNA m(6)A37 methyltransferase
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
JC JC 3,651,029 IS2 (+) +5 bp 91.4% intergenic (+367/‑258) arsC → / → yhiS arsenate reductase/putative uncharacterized protein YhiS
RA 3,991,328 A→T 11.9% K59I (AAA→ATA)  cyaA → adenylate cyclase
RA 4,182,820 C→T 32.4% H526Y (CAC→TAC)  rpoB → RNA polymerase subunit beta
RA 4,183,379 C→T 62.6% S712F (TCC→TTC)  rpoB → RNA polymerase subunit beta
RA 4,296,060 C→T 44.6% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423895–3424234 3424496–3424239 6–602 7 [6] [5] 10 rrlD 23S ribosomal RNA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 151467 =31 (0.990)4 (0.130) 3/400 NT 13.1% coding (133/597 nt) yadK putative fimbrial protein YadK
?NC_000913 151543 = 23 (0.760)coding (57/597 nt) yadK putative fimbrial protein YadK
* ? NC_000913 1207790 =21 (0.670)7 (0.240) 7/382 NT 25.3% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 22 (0.770)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780521 (0.670)21 (0.730) 19/382 NT 50.4% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 22 (0.770)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 3717730 =31 (0.990)4 (0.130) 4/394 NT 12.4% coding (421/975 nt) ghrB glyoxylate reductase
?NC_000913 = 3919436 27 (0.910)coding (422/1542 nt) atpA ATP synthase F1 complex subunit alpha