breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT43.7% 53.7 / 50.8 71S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009132,001,9180AC37.2% 61.7 / 17.7 43intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
*NC_0009133,556,2050AC34.4% 48.4 / 11.4 32S215R (AGT→AGG) ‡rtcARNA 3'‑terminal phosphate cyclase
*NC_0009131,377,1400AC33.3% 114.0 / 19.3 63K103N (AAA→AACycjSputative oxidoreductase YcjS
*NC_0009134,101,5070TG33.3% 96.9 / 10.8 57intergenic (+77/‑183)sodA/kdgTsuperoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
*NC_0009134,111,7470GT33.3% 85.2 / 27.3 42N156K (AAC→AAAyiiQDUF1454 domain‑containing protein YiiQ
*NC_0009134,213,0510AC33.3% 6.3 / 10.7 12noncoding (12/120 nt)rrfE5S ribosomal RNA
*NC_0009133,556,2070TC31.4% 54.1 / 10.3 35S215G (AGT→GGT) ‡rtcARNA 3'‑terminal phosphate cyclase
*NC_0009134,101,5140GT31.0% 131.5 / 27.5 58intergenic (+84/‑176)sodA/kdgTsuperoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
*NC_0009134,101,5250CT30.2% 112.8 / 13.9 63intergenic (+95/‑165)sodA/kdgTsuperoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
*NC_0009132,326,4500TG28.9% 150.7 / 47.0 83G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_0009134,568,5040TG28.6% 209.8 / 14.2 105T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
*NC_000913650,8090TG28.4% 142.0 / 12.0 68T350P (ACC→CCC) citCcitrate lyase synthetase
*NC_0009133,206,4350CT27.8% 92.3 / 23.1 55intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
*NC_0009133,401,4590TC27.8% 136.8 / 16.7 72D625G (GAT→GGT) csrDregulator of CsrB and CsrC decay
*NC_0009133,206,4440TA26.4% 112.7 / 15.5 53intergenic (‑188/‑19)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
*NC_0009134,296,0600CT26.2% 140.7 / 52.8 80intergenic (+266/+376)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_000913753,1250TA25.4% 143.4 / 17.6 59intergenic (‑330/+60)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
*NC_00091329,4960TA24.5% 114.0 / 12.5 49intergenic (+301/‑155)dapB/carA4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl phosphate synthetase subunit alpha
*NC_000913175,6940TG24.3% 160.1 / 18.1 74G196G (GGT→GGGclcAchloride:H(+) antiporter ClcA

Marginal new junction evidence (lowest skew 10 of 46 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 375124 =NA (NA)20 (0.300) 9/192 4.2 NA intergenic (+243/‑335) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377530 = NA (NA)intergenic (+219/+5) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 4542682 =65 (0.680)13 (0.150) 12/258 4.6 14.8% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 89 (1.000)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269054 (0.570)10 (0.110) 10/258 5.1 12.8% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 86 (0.960)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 608007 =NA (NA)14 (0.180) 8/224 5.1 14.4% noncoding (1/1345 nt) IS186 repeat region
?NC_000913 609352 = 83 (1.070)intergenic (+175/+107) insL‑2/entD IS186/IS421 transposase/phosphopantetheinyl transferase EntD
* ? NC_000913 374922 =NA (NA)13 (0.190) 7/202 5.1 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377430 = NA (NA)intergenic (+119/+105) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375023 =NA (NA)8 (0.120) 6/192 5.2 NA intergenic (+142/‑436) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377530 = NA (NA)intergenic (+219/+5) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 35923677 (0.810)10 (0.120) 9/246 5.2 11.4% coding (438/660 nt) cynT carbonic anhydrase 1
?NC_000913 = 359364 87 (1.020)coding (566/660 nt) cynT carbonic anhydrase 1
* ? NC_000913 = 375059NA (NA)18 (0.190)
+T
10/274 5.4 NA noncoding (28/34 nt) other REP31c
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 = 375246NA (NA)13 (0.150)
+GAACGCCTTATCCGGT
8/244 5.5 NA noncoding (13/34 nt) other REP31g
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 = 375160NA (NA)27 (0.280)
+T
9/274 5.7 NA noncoding (28/34 nt) other REP31e
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d