breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT39.4% 108.9 / 73.4 104S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009133,943,4880TG37.1% 29.8 / 12.4 35noncoding (54/76 nt)gltUtRNA‑Glu
*NC_000913753,1250TA33.0% 217.4 / 54.5 115intergenic (‑330/+60)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
*NC_0009133,556,2050AC30.4% 88.2 / 17.2 46S215R (AGT→AGG) ‡rtcARNA 3'‑terminal phosphate cyclase
*NC_0009134,568,5040TG29.8% 260.3 / 25.8 138T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
*NC_0009133,556,2070TC29.8% 78.5 / 15.7 47S215G (AGT→GGT) ‡rtcARNA 3'‑terminal phosphate cyclase
*NC_0009134,209,8270TG27.5% 61.9 / 13.3 40noncoding (54/76 nt)gltVtRNA‑Glu
*NC_0009132,001,9180AC27.4% 199.6 / 39.6 95intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
*NC_0009131,825,0610GA27.0% 71.8 / 11.0 37intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
*NC_0009132,326,4500TG25.7% 148.1 / 44.8 74G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_0009134,296,0600CT25.4% 276.4 / 88.8 138intergenic (+266/+376)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_000913445,5580AC25.0% 91.8 / 14.8 44V370G (GTA→GGA) yajRputative major facilitator superfamily transport protein YajR
*NC_0009134,429,9840TG25.0% 190.8 / 29.3 84L41V (TTG→GTG) cycAserine/alanine/glycine/cycloserine:H(+)symporter
*NC_0009131,775,4670AC24.5% 215.7 / 13.8 94intergenic (+23/‑120)aroD/ydiF3‑dehydroquinate dehydratase/putative acetate‑CoA transferase
*NC_0009134,111,7470GT24.0% 211.6 / 28.5 75N156K (AAC→AAAyiiQDUF1454 domain‑containing protein YiiQ
*NC_000913646,7470TG23.7% 169.7 / 33.6 76T255P (ACC→CCC) citGtriphosphoribosyl‑dephospho‑CoA synthase
*NC_000913650,8090TG23.6% 164.3 / 15.4 72T350P (ACC→CCC) citCcitrate lyase synthetase
*NC_000913372,6400TG23.6% 210.5 / 24.1 89L176V (TTA→GTA) mhpD2‑hydroxypentadienoate hydratase
*NC_00091329,4960TA23.5% 187.7 / 31.0 81intergenic (+301/‑155)dapB/carA4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl phosphate synthetase subunit alpha
*NC_0009133,531,4400AC20.9% 264.3 / 20.8 106Y334D (TAT→GAT) yhgEputative transport protein YhgE

Marginal new junction evidence (lowest skew 10 of 14 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4542690125 (1.020)28 (0.240) 24/258 3.4 19.1% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 120 (1.050)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 4542682 =118 (0.960)15 (0.130) 13/258 6.0 11.5% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 121 (1.060)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 374963NA (NA)5 (0.060) 5/190 7.4 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 374922 =NA (NA)11 (0.100) 5/260 9.3 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377329 = NA (NA)intergenic (+18/+206) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 1979486 =164 (1.340)30 (0.240) 5/276 9.7 15.5% intergenic (‑271/‑267) insA‑5/uspC IS1 protein InsA/universal stress protein C
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 340035 =NA (NA)7 (0.060) 5/274 9.7 NA coding (243/297 nt) yahH putative uncharacterized protein YahH
?NC_000913 377322 = NA (NA)intergenic (+11/+213) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 1979633160 (1.310)22 (0.180) 4/276 10.3 12.1% intergenic (‑418/‑120) insA‑5/uspC IS1 protein InsA/universal stress protein C
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 52326583 (0.680)6 (0.050) 4/276 10.3 7.9% coding (5/4281 nt) rhsD protein RhsD
?NC_000913 = 523314 57 (0.470)coding (54/4281 nt) rhsD protein RhsD
* ? NC_000913 1462089 =106 (0.860)4 (0.040) 3/252 10.4 3.0% coding (1197/1206 nt) paaJ beta‑ketoadipyl‑CoA thiolase
?NC_000913 1462223 = 164 (1.470)coding (99/1314 nt) paaK phenylacetate‑CoA ligase
* ? NC_000913 = 4373296NA (NA)3 (0.030) 3/256 10.5 NA noncoding (1/34 nt) other REP328b
?NC_000913 = 4374496 NA (NA)intergenic (‑262/+133) yjeJ/epmB protein YjeJ/lysine 2,3‑aminomutase