breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 533,562 Δ2 bp coding (552‑553/816 nt) allR → DNA‑binding transcriptional repressor AllR
RA 741,485 C→A G358* (GGA→TGA)  dtpD ← dipeptide:H(+) symporter DtpD
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
MC JC 1,230,159 Δ12 bp coding (225‑236/912 nt) hlyE ← hemolysin E
RA 1,290,622 C→A S127R (AGC→AGA rssB → regulator of RpoS
RA 1,526,385 C→A L47M (CTG→ATG)  yncG → putative glutathione S‑transferase YncG
RA 1,631,653 C→A Q211H (CAG→CAT ydfJ ← putative transporter YdfJ
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,231,994 G→T A51S (GCG→TCG)  cdd → cytidine/deoxycytidine deaminase
RA 2,673,153 G→T G369G (GGG→GGT hcaD → putative 3‑phenylpropionate/cinnamate dioxygenase ferredoxin reductase subunit
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,440,436 C→T L198L (CTG→CTA rpoA ← RNA polymerase subunit alpha
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,648,648 G→T D41Y (GAC→TAC)  arsR → DNA‑binding transcriptional repressor ArsR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,878,834 C→A G429V (GGC→GTC)  gyrB ← DNA gyrase subunit B
RA 4,040,668 G→T intergenic (+32/+238) rrfA → / ← mobB 5S ribosomal RNA/molybdopterin‑guanine dinucleotide biosynthesis adaptor protein
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102943 3104087 1145 44 [43] [43] 45 mutY mutY
* * ÷ NC_000913 3423755–3424233 3424536–3424239 7–782 44 [42] [39] 45 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =3 (0.020)150 (1.380) 76/244 0.0 97.5% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 5 (0.050)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078053 (0.020)75 (0.690) 46/244 0.8 95.2% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 5 (0.050)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)137 (1.150) 75/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =226 (1.840)176 (1.450) 78/274 0.1 60.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)106 (0.860) 59/276 0.5 95.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA