breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 486,332 C→A A191D (GCC→GAC)  acrR → DNA‑binding transcriptional repressor AcrR
RA 522,001 C→A A529E (GCG→GAG)  ybbP → putative ABC transporter membrane subunit YbbP
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,202,729 T→A G817G (GGT→GGA yehI → DUF4132 domain‑containing protein YehI
RA 2,535,867 Δ1 bp coding (34/510 nt) crr → Enzyme IIA(Glc)
RA 2,636,245 G→T A371E (GCG→GAG)  der ← 50S ribosomal subunit stability factor
RA 2,881,332 C→A A271S (GCC→TCC)  casC ← type I‑E CRISPR system Cascade subunit CasC
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,216,538 C→A S354* (TCG→TAG)  aceB → malate synthase A
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102965 3104083 1119 45 [44] [44] 46 mutY mutY
* * ÷ NC_000913 3423755–3424234 3424550–3424239 6–796 45 [43] [44] 45 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =14 (0.100)155 (1.310) 77/244 0.1 93.5% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 9 (0.080)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780514 (0.100)86 (0.730) 61/244 0.4 88.9% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 9 (0.080)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)174 (1.340) 89/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =212 (1.580)192 (1.440) 87/274 0.1 64.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)128 (0.960) 77/276 0.2 95.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 6 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 4542690106 (0.790)36 (0.290) 30/258 2.9 29.4% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 74 (0.590)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit