breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 435,813 T→A D60E (GAT→GAA thiL → thiamine monophosphate kinase
RA 571,663 T→C Y70Y (TAT→TAC ybcM → DLP12 prophage; putative DNA‑binding transcriptional regulator
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,052,605 T→G D253A (GAC→GCC)  yccM ← putative 4Fe‑4S membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,622,811 A→G intergenic (+70/+6) mgtS → / ← mgrR small protein MgtS/small regulatory RNA MgrR
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,490,314 G→A A546V (GCA→GTA)  oxc ← oxalyl‑CoA decarboxylase
RA 2,494,424 T→C intergenic (‑22/‑274) yfdX ← / → ypdI protein YfdX/colanic acid synthesis putative lipoprotein YpdI
RA 2,626,944 C→T L665L (CTG→CTA pdeF ← cyclic di‑GMP phosphodiesterase PdeF
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196259 1211428 15170 15 [14] [12] 16 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423766–3424233 3424544–3424238 6–779 15 [12] [14] 16 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)71 (1.230) 42/268 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =92 (1.550)104 (1.770) 60/274 0.0 69.0% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)77 (1.300) 53/276 0.1 96.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =53 (0.890)14 (0.250) 12/258 2.8 20.4% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 60 (1.080)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit