breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT41.6% 144.0 / 94.9 137S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009133,206,4350CT34.9% 84.1 / 37.6 63intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
*NC_0009131,825,0610GA32.8% 104.6 / 28.9 67intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
*NC_0009134,568,5040TG32.3% 357.2 / 36.0 199T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
*NC_0009133,206,4440TA30.6% 106.2 / 22.6 62intergenic (‑188/‑19)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
*NC_0009131,775,4670AC28.8% 141.0 / 10.7 73intergenic (+23/‑120)aroD/ydiF3‑dehydroquinate dehydratase/putative acetate‑CoA transferase
*NC_0009132,667,1260AC27.4% 220.7 / 28.4 113Y241D (TAT→GAT) hcaTputative 3‑phenylpropionate transporter
*NC_000913501,5750AC27.4% 122.7 / 23.3 62V555G (GTG→GGG) ybaLputative transporter YbaL
*NC_0009134,101,5070TG27.1% 196.4 / 13.9 96intergenic (+77/‑183)sodA/kdgTsuperoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
*NC_0009134,628,6490AC26.8% 156.2 / 10.5 71intergenic (+102/+206)nadR/ettADNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA
*NC_000913650,8090TG26.0% 204.1 / 18.8 100T350P (ACC→CCC) citCcitrate lyase synthetase
*NC_0009132,326,4500TG25.7% 132.4 / 40.0 70G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_000913372,6400TG25.4% 263.5 / 27.9 122L176V (TTA→GTA) mhpD2‑hydroxypentadienoate hydratase
*NC_000913501,5640AC25.4% 136.7 / 29.4 67*559E (TAA→GAA) ybaLputative transporter YbaL
*NC_0009134,101,5140GT24.2% 254.2 / 39.0 95intergenic (+84/‑176)sodA/kdgTsuperoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
*NC_0009133,556,2070TC23.7% 118.5 / 14.8 59S215G (AGT→GGT) rtcARNA 3'‑terminal phosphate cyclase
*NC_0009132,001,9180AC23.0% 201.3 / 23.9 87intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
*NC_0009134,098,1550AC23.0% 295.6 / 19.6 135L140F (TTA→TTCrhaSDNA‑binding transcriptional activator RhaS
*NC_000913888,0140TG22.7% 202.7 / 11.3 89noncoding (44/81 nt)rybBsmall regulatory RNA RybB
*NC_0009134,111,7470GT22.2% 153.7 / 16.5 54N156K (AAC→AAAyiiQDUF1454 domain‑containing protein YiiQ

Marginal new junction evidence (lowest skew 10 of 17 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1207805100 (0.740)24 (0.200) 20/246 4.1 23.0% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 72 (0.600)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 1207790 =119 (0.880)18 (0.150) 16/246 5.0 16.7% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 74 (0.620)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 454269093 (0.690)9 (0.070) 9/260 7.4 8.8% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 99 (0.780)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 608007 =NA (NA)15 (0.140) 7/226 7.4 10.4% noncoding (1/1345 nt) IS186 repeat region
?NC_000913 609352 = 129 (1.180)intergenic (+175/+107) insL‑2/entD IS186/IS421 transposase/phosphopantetheinyl transferase EntD
* ? NC_000913 4542682 =101 (0.750)7 (0.060) 7/260 8.2 6.8% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 99 (0.780)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 2728258 =NA (NA)8 (0.060) 7/264 8.3 NA noncoding (927/2904 nt) rrlG 23S ribosomal RNA
?NC_000913 2728336 = NA (NA)noncoding (849/2904 nt) rrlG 23S ribosomal RNA
* ? NC_000913 = 22668552 (0.380)8 (0.060) 7/264 8.3 14.0% noncoding (927/2904 nt) rrlH 23S ribosomal RNA
?NC_000913 2728336 = NA (NA)noncoding (849/2904 nt) rrlG 23S ribosomal RNA
* ? NC_000913 1992602 =125 (0.920)4 (0.030) 4/258 9.7 3.1% coding (216/549 nt) pgsA phosphatidylglycerophosphate synthase
?NC_000913 1992748 = 131 (1.040)coding (70/549 nt) pgsA phosphatidylglycerophosphate synthase
* ? NC_000913 = 3660514170 (1.260)4 (0.030) 4/264 9.8 2.5% coding (101/3114 nt) mdtF multidrug efflux pump RND permease MdtF
?NC_000913 = 3660571 157 (1.220)coding (158/3114 nt) mdtF multidrug efflux pump RND permease MdtF
* ? NC_000913 2066566 =37 (0.270)4 (0.030) 4/268 9.9 10.1% noncoding (788/1195 nt) IS5 repeat region
?NC_000913 2102304 = NA (NA)noncoding (640/1195 nt) IS5 repeat region