breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
RA BN000925 9,791 A→G D797G (GAC→GGC)  tnpA → Tn1 transposase
RA BN000925 9,802 G→A D801N (GAC→AAC)  tnpA → Tn1 transposase
RA BN000925 10,089 A→C A896A (GCA→GCC tnpA → Tn1 transposase
RA BN000925 10,359 T→A G986G (GGT→GGA tnpA → Tn1 transposase
RA BN000925 12,239 +A intergenic (‑601/+1055) klcA ← / ← tetR KlcA putative anti‑restriction protein/tetracycline resistance repressor protein
RA BN000925 12,406 T→G intergenic (‑768/+888) klcA ← / ← tetR KlcA putative anti‑restriction protein/tetracycline resistance repressor protein
RA BN000925 12,688 T→A intergenic (‑1050/+606) klcA ← / ← tetR KlcA putative anti‑restriction protein/tetracycline resistance repressor protein
RA BN000925 14,765 A→G D239G (GAT→GGT)  tetA → tetracycline exporter protein
RA BN000925 15,561 Δ1 bp intergenic (+312/+320) tetA → / ← upf16.5 tetracycline exporter protein/hypothetical protein
RA BN000925 15,639 C→G intergenic (+390/+242) tetA → / ← upf16.5 tetracycline exporter protein/hypothetical protein
RA BN000925 15,898 +C coding (649/666 nt) upf16.5 ← hypothetical protein
RA BN000925 16,410 +T coding (137/666 nt) upf16.5 ← hypothetical protein
RA BN000925 31,028 C→G P35A (CCC→GCC)  trbP → TrbP protein
RA BN000925 36,655 A→G R116G (AGA→GGA) 
R108G (AGA→GGA) 
istA →
istA →
IS21 transposase
IS21 transposase
RA BN000925 38,388 A→C pseudogene (45/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,390 1 bp→AG pseudogene (47/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,393 C→T pseudogene (50/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,421 Δ1 bp pseudogene (78/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,510 Δ1 bp pseudogene (167/171 nt) upf35.8 → probable truncation of theoretical product due to sequence errors
RA BN000925 38,526 Δ1 bp intergenic (+12/‑232) upf35.8 → / → aphA probable truncation of theoretical product due to sequence errors/aminoglycoside 3'‑phospho‑transferase
RA BN000925 39,136 Δ1 bp coding (379/774 nt) aphA → aminoglycoside 3'‑phospho‑transferase
RA BN000925 39,147 Δ1 bp coding (390/774 nt) aphA → aminoglycoside 3'‑phospho‑transferase
RA BN000925 39,179 +CG coding (422/774 nt) aphA → aminoglycoside 3'‑phospho‑transferase
RA BN000925 53,069 +T intergenic (+42/+10) traM → / ← kfrC TraM protein/KfrC protein
RA BN000925 54,833 Δ1 bp intergenic (‑32/+127) kfrB ← / ← kfrA KfrB protein/KfrA coiled‑coil protein
RA BN000925 56,817 Δ1 bp coding (297/522 nt) korF ← KorF repressor protein
MC JC NC_000913 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
JC JC NC_000913 986,348 IS1 (–) +9 bp coding (627‑635/1089 nt) ompF ← outer membrane porin F
RA NC_000913 1,258,727 A→G intergenic (‑214/‑64) pth ← / → ychH peptidyl‑tRNA hydrolase/stress‑induced protein
RA NC_000913 1,296,375 A→G F583L (TTC→CTC)  adhE ← aldehyde‑alcohol dehydrogenase
RA NC_000913 1,298,371 C→A intergenic (‑250/‑227) adhE ← / → ychE aldehyde‑alcohol dehydrogenase/putative inner membrane protein
MC JC NC_000913 1,299,499 Δ1,199 bp insH21 insH21
MC JC NC_000913 1,398,001 Δ29,480 bp [uspE][lomR] 41 genes
JC JC NC_000913 2,042,465 IS5 (+) +4 bp coding (98‑101/531 nt) yodB → putative cytochrome
RA NC_000913 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA NC_000913 2,249,587 A→T F11Y (TTT→TAT)  yeiE ← putative LysR‑type transcriptional regulator YeiE
RA NC_000913 2,700,256 Δ1 bp intergenic (‑59/‑264) shoB ← / → ohsC toxic peptide ShoB/small regulatory RNA OhsC
RA NC_000913 3,213,531 T→G I162S (ATC→AGC)  rpoD → RNA polymerase, sigma 70 (sigma D) factor
MC JC NC_000913 3,366,751 Δ295 bp IS5‑mediated yhcE[yhcF] yhcE, [yhcF]
RA NC_000913 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA NC_000913 3,717,751 C→T L148L (CTG→TTG)  ghrB → glyoxylate reductase
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 300050 300185 136 6 [5] [5] 6 paoC aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
* * ÷ NC_000913 423465 423578 114 8 [5] [4] 6 malZ maltodextrin glucosidase
* * ÷ NC_000913 907488 907644 157 6 [5] [5] 7 ybjT putative NAD(P)‑dependent oxidoreductase YbjT
* * ÷ NC_000913 3423865–3424232 3424447–3424239 8–583 6 [5] [5] 6 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 36026315 (0.500)4 (0.140) 4/398 3.0 22.1% coding (272/1155 nt) cynX cyanate transporter
?NC_000913 = 360267 14 (0.490)coding (276/1155 nt) cynX cyanate transporter
* ? NC_000913 = 8073360 (0.000)17 (0.570)
+G
15/418 0.9 55.0% intergenic (‑55/+97) ybhC/ybhB outer membrane lipoprotein YhbC/putative kinase inhibitor
?NC_000913 = 4171849 28 (0.930)intergenic (+93/‑208) rrfB/murB 5S ribosomal RNA/UDP‑N‑acetylenolpyruvoylglucosamine reductase
* ? NC_000913 1207790 =13 (0.430)19 (0.680) 14/388 0.9 63.3% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 10 (0.360)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780513 (0.430)26 (0.930) 23/388 0.3 70.3% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 10 (0.360)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment