breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 188,837 C→T R316C (CGC→TGC)  PP_0165 → GGDEF domain‑containing protein
RA 233,545 C→T G548G (GGC→GGT PP_0180 → cytochrome c family protein
RA 476,133 A→T Q141L (CAG→CTG)  tsaD → tRNA N6‑adenosine(37)‑threonylcarbamoyltransferase complex transferase subunit TsaD
RA 1,011,323 +T intergenic (‑258/+192) PP_0871 ← / ← SBRG_Ecoli_galM glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM
RA 1,013,189 C→G C170S (TGC→TCC)  SBRG_Ecoli_galK ← SBRG_Ecoli_galK
RA 1,015,820 A→C intergenic (‑47/‑721) SBRG_Ecoli_galE ← / → prfC SBRG_Ecoli_galE/peptide chain release factor 3
RA 1,165,540 C→T L133F (CTC→TTC)  gtsC → mannose/glucose ABC transporter permease
RA 1,165,856 C→T T238I (ACC→ATC)  gtsC → mannose/glucose ABC transporter permease
RA 1,168,596 G→T intergenic (+65/‑53) oprB‑I → / → yeaD carbohydrate‑selective porin/glucose‑6‑phosphate 1‑epimerase
MC JC 1,515,811 (33-bp)2→1 pseudogene (715‑747/1851 nt) PP_1325 ← putative lipoprotein
RA 1,856,513 A→G D203G (GAT→GGT)  relA → ATP:GTP 3'‑pyrophosphotransferase
RA 2,209,475 A→T N387I (AAC→ATC)  PP_1948 → benzaldehyde dehydrogenase
RA 2,293,757 G→A D350N (GAC→AAC)  pepN → aminopeptidase N
RA 2,384,391 C→A Q227K (CAA→AAA)  cmpX → protein CmpX
RA 4,018,201 G→A intergenic (+144/‑36) mvaB → / → PP_mr44 hydroxymethylglutaryl‑CoA lyase/CrcZ ncRNA
RA 4,746,222 +C intergenic (+464/+288) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,224 A→G intergenic (+466/+286) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,226 C→T intergenic (+468/+284) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,229 C→T intergenic (+471/+281) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,232 C→G intergenic (+474/+278) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,234 2 bp→CT intergenic (+476/+275) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,248 C→T intergenic (+490/+262) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,250 A→C intergenic (+492/+260) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,746,253 2 bp→GC intergenic (+495/+256) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,953,768 Δ1 bp coding (74/435 nt) fliO ← flagellar protein FliO

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 = 746964NA (NA)380 (0.910) 150/284 1.0 88.8% intergenic (‑88/‑706) PP_0638/PP_5448 transposase/hypothetical protein
?NC_002947 = 1015874 48 (0.120)intergenic (‑101/‑667) SBRG_Ecoli_galE/prfC SBRG_Ecoli_galE/peptide chain release factor 3
* ? NC_002947 1015867 =18 (0.040)457 (1.110) 182/282 0.1 70.4% intergenic (‑94/‑674) SBRG_Ecoli_galE/prfC SBRG_Ecoli_galE/peptide chain release factor 3
?NC_002947 5406744 = 367 (0.890)intergenic (+1123/+45) PP_4743/PP_4745 hypothetical protein/transposase
* ? NC_002947 1849125 =4 (0.010)430 (1.070) 167/274 0.3 65.8% coding (660/2754 nt) gacS sensor protein GacS
?NC_002947 = 2184368 443 (1.100)intergenic (+175/+93) PP_1931/PP_5486 hypothetical protein/hypothetical protein
* ? NC_002947 = 18491284 (0.010)365 (0.900) 166/276 0.3 65.1% coding (657/2754 nt) gacS sensor protein GacS
?NC_002947 2182694 = 388 (0.960)intergenic (+103/‑63) PP_5485/PP_1931 GNAT family acetyltransferase/hypothetical protein