breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE707,9100AG74.2% 98.5 / 40.5 62intergenic (‑1/‑121)znuC_1/ideRHigh‑affinity zinc uptake system ATP‑binding protein ZnuC/Iron‑dependent repressor IdeR
*USA300TCH1516_ALE1,233,5040TA73.1% 99.8 / 38.7 52V470D (GTT→GAT) prkCSerine/threonine‑protein kinase PrkC
*USA300TCH1516_ALE18,8900CG72.9% 92.3 / 40.6 48N182K (AAC→AAGgdpPCyclic‑di‑AMP phosphodiesterase GdpP
*USA300TCH1516_ALE512,6430AG51.1% 1.9 / 212.1 137intergenic (+711/‑6052)recR/speA_2Recombination protein RecR/Arginine decarboxylase
*USA300TCH1516_ALE111,5160CA39.4% 103.4 / 103.7 94Q196K (CAA→AAA) USA300HOU_RS00520putative lipoprotein
*USA300TCH1516_ALE111,5120AG39.0% 87.2 / 103.7 96S194S (TCA→TCGUSA300HOU_RS00520putative lipoprotein
*USA300TCH1516_ALE2,026,6550CT29.5% 82.1 / 49.0 61E87K (GAA→AAA) vraRResponse regulator protein VraR
*USA300TCH1516_ALE707,8790AT23.8% 128.7 / 46.6 63L11I (TTA→ATA) znuC_1High‑affinity zinc uptake system ATP‑binding protein ZnuC
*USA300TCH1516_ALE479,5530GA23.7% 111.1 / 35.8 59A194T (GCA→ACA) USA300HOU_RS02280hypothetical protein
*USA300TCH1516_ALE367,6440GT22.4% 183.2 / 40.3 76intergenic (‑96/‑62)nanA/bglKN‑acetylneuraminate lyase/Beta‑glucoside kinase
*USA300TCH1516_ALE1,797,7720GC22.0% 119.5 / 33.5 50R7G (CGC→GGC) phoRAlkaline phosphatase synthesis sensor protein PhoR
*USA300TCH1516_ALE1,419,5270GT21.1% 120.2 / 23.9 52P7T (CCT→ACT) USA300HOU_RS07040hypothetical protein
*USA300TCH1516_ALE1,744,4540CT21.1% 114.1 / 25.9 57G73D (GGC→GAC) aptAdenine phosphoribosyltransferase

Marginal new junction evidence (lowest skew 10 of 106 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000731 = 44332 (0.610)13 (0.010) 5/270 0.1 1.3% intergenic (+398/‑67) USA300HOU_pUSA300HOUMR0006/cadR hypothetical protein/cadmium binding protein
?NC_012417 = 1931 1958 (0.870)intergenic (+164/+162) USA300HOU_RS14895/USA300HOU_RS14900 hypothetical protein/hypothetical protein
* ? CP000731 = 44192 (0.610)30 (0.030) 11/264 0.1 2.9% intergenic (+384/‑81) USA300HOU_pUSA300HOUMR0006/cadR hypothetical protein/cadmium binding protein
?NC_012417 = 1946 1982 (0.900)intergenic (+179/+147) USA300HOU_RS14895/USA300HOU_RS14900 hypothetical protein/hypothetical protein
* ? CP000731 = 43672 (0.610)42 (0.040) 13/268 0.1 4.0% intergenic (+332/‑133) USA300HOU_pUSA300HOUMR0006/cadR hypothetical protein/cadmium binding protein
?NC_012417 = 1991 2020 (0.900)intergenic (+224/+102) USA300HOU_RS14895/USA300HOU_RS14900 hypothetical protein/hypothetical protein
* ? CP000731 4323 =1 (0.310)19 (0.020) 13/266 0.1 1.9% intergenic (+288/‑177) USA300HOU_pUSA300HOUMR0006/cadR hypothetical protein/cadmium binding protein
?NC_012417 2035 = 1948 (0.880)intergenic (+268/+58) USA300HOU_RS14895/USA300HOU_RS14900 hypothetical protein/hypothetical protein
* ? CP000731 4294 =4 (1.220)56 (0.050) 21/264 0.1 5.7% intergenic (+259/‑206) USA300HOU_pUSA300HOUMR0006/cadR hypothetical protein/cadmium binding protein
?NC_012417 2073 = 1861 (0.840)intergenic (+306/+20) USA300HOU_RS14895/USA300HOU_RS14900 hypothetical protein/hypothetical protein
* ? USA300TCH1516_ALE = 203178570 (1.120)4 (0.110)
+59 bp
3/162 7.2 9.9% intergenic (+251/+239) USA300HOU_RS10250/USA300HOU_RS10255 hypothetical protein/hypothetical protein
?USA300TCH1516_ALE = 2866882 56 (0.890)intergenic (‑281/+172) USA300HOU_RS14695/noc_2 hypothetical protein/Nucleoid occlusion protein
* ? USA300TCH1516_ALE = 203178570 (1.120)4 (0.110)
+59 bp
3/162 7.2 9.4% intergenic (+251/+239) USA300HOU_RS10250/USA300HOU_RS10255 hypothetical protein/hypothetical protein
?USA300TCH1516_ALE = 2031834 63 (1.000)intergenic (+300/+190) USA300HOU_RS10250/USA300HOU_RS10255 hypothetical protein/hypothetical protein
* ? USA300TCH1516_ALE = 147005869 (1.100)4 (0.110)
+59 bp
3/162 7.2 9.0% intergenic (‑105/‑240) USA300TCH1516_01369/rnhA hypothetical protein/14.7 kDa ribonuclease H‑like protein
?USA300TCH1516_ALE = 2031785 70 (1.120)intergenic (+251/+239) USA300HOU_RS10250/USA300HOU_RS10255 hypothetical protein/hypothetical protein
* ? USA300TCH1516_ALE 1380507 =54 (0.860)4 (0.110)
+59 bp
3/162 7.2 10.0% intergenic (+321/‑571) opuD_1/acnA Glycine betaine transporter OpuD/Aconitate hydratase A
?USA300TCH1516_ALE = 2031785 70 (1.120)intergenic (+251/+239) USA300HOU_RS10250/USA300HOU_RS10255 hypothetical protein/hypothetical protein
* ? USA300TCH1516_ALE = 109823559 (0.940)4 (0.110)
+59 bp
3/162 7.2 9.7% intergenic (+214/+135) ktrA/rnj1 Ktr system potassium uptake protein A/Ribonuclease J 1
?USA300TCH1516_ALE = 2031785 70 (1.120)intergenic (+251/+239) USA300HOU_RS10250/USA300HOU_RS10255 hypothetical protein/hypothetical protein