breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 348 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913941,7130TG52.2% 51.4 / 73.8 46V252G (GTG→GGG) dmsAdimethyl sulfoxide reductase, anaerobic, subunit A
*NC_000913357,8800TC50.9% 57.7 / 84.5 55K271E (AAA→GAA) cynRtranscriptional activator of cyn operon; autorepressor
*NC_000913925,9000AG49.1% 67.7 / 14.6 53noncoding (72/88 nt)serWtRNA‑Ser
*NC_000913987,4940TG48.5% 100.0 / 19.0 66intergenic (‑512/+91)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
*NC_0009134,378,8020AC48.5% 104.0 / 21.1 66V48G (GTA→GGA) ampCpenicillin‑binding protein; beta‑lactamase, intrinsically weak
*NC_0009132,248,4310AC48.3% 81.0 / 22.7 60G34G (GGT→GGGlysPlysine transporter
*NC_0009132,421,4430AC47.8% 97.5 / 21.6 67Y40S (TAC→TCC) folXD‑erythro‑7,8‑dihydroneopterin triphosphate 2'‑epimerase and dihydroneopterin aldolase
*NC_0009133,206,4350CT47.5% 61.1 / 30.9 59intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
*NC_0009131,079,4680AG45.3% 69.1 / 17.2 53D55G (GAT→GGT) putPproline:sodium symporter
*NC_000913357,8900CA45.1% 36.5 / 27.9 52L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_0009133,355,4570AC45.1% 104.6 / 19.8 71T245P (ACC→CCC) gltBglutamate synthase, large subunit
*NC_0009131,312,2100CA45.0% 105.4 / 21.5 60V13F (GTT→TTT) yciAacyl‑CoA esterase
*NC_000913504,6400CT44.4% 57.6 / 31.5 64K19K (AAG→AAAfsrputative fosmidomycin efflux system protein
*NC_0009131,376,5050AC44.4% 82.9 / 17.1 54T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
*NC_0009131,312,2270AG44.3% 94.7 / 17.3 61V7A (GTC→GCC) yciAacyl‑CoA esterase
*NC_0009132,903,6920CA43.1% 87.3 / 40.0 58intergenic (+318/+1055)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
*NC_0009133,001,1640AC42.4% 92.1 / 20.2 59T274P (ACC→CCC) xdhAxanthine dehydrogenase, molybdenum binding subunit
*NC_00091373,5810AC42.3% 83.0 / 12.7 52V314G (GTA→GGA) thiPthiamine/thiamine pyrophosphate ABC transporter permease
*NC_0009134,604,3440CT42.3% 53.8 / 23.8 52S62F (TCT→TTT) bglJbgl operon transcriptional activator
*NC_000913993,9170AC42.0% 119.0 / 20.2 69V43G (GTG→GGG) ssuBaliphatic sulfonate ABC transporter ATPase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 372775 =69 (1.050)4 (0.060) 4/292 12.1 5.4% coding (661/810 nt) mhpD 2‑keto‑4‑pentenoate hydratase
?NC_000913 372789 = 73 (1.130)coding (675/810 nt) mhpD 2‑keto‑4‑pentenoate hydratase