breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 180 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8900CA60.5% ‑0.4 / 29.4 39L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_000913357,8800TC54.8% 27.6 / 53.6 43K271E (AAA→GAA) cynRtranscriptional activator of cyn operon; autorepressor
*NC_000913357,8750AT50.0% 34.0 / 22.0 45N272K (AAT→AAAcynRtranscriptional activator of cyn operon; autorepressor
*NC_0009132,151,5890AT48.1% 50.7 / 32.6 56L135F (TTA→TTTyegJuncharacterized protein
*NC_0009132,151,5920GT46.4% 56.8 / 34.9 56L136F (TTG→TTTyegJuncharacterized protein
*NC_0009132,096,0900AG46.3% 40.4 / 11.0 41I83V (ATT→GTT) hisFimidazole glycerol phosphate synthase, catalytic subunit with HisH
*NC_0009134,332,7910AC43.9% 94.2 / 17.8 57V161G (GTG→GGG) basSsensory histidine kinase in two‑component regulatory system with BasR
*NC_0009133,206,4350CT43.4% 43.2 / 29.8 53intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
*NC_0009132,151,6080GA43.1% 47.6 / 28.5 51E142K (GAA→AAA) yegJuncharacterized protein
*NC_0009131,397,8300TC41.7% 69.3 / 18.4 61I265V (ATT→GTT) uspEstress‑induced protein
*NC_0009131,622,8160GA41.7% 34.8 / 42.0 48intergenic (+75/+144)yneM/dgcZinner membrane‑associated protein/diguanylate cyclase, zinc‑sensing
*NC_0009134,604,3440CT40.3% 70.2 / 32.0 63S62F (TCT→TTT) bglJbgl operon transcriptional activator
*NC_000913357,8730CT40.0% 36.5 / 18.8 45S273N (AGC→AAC) cynRtranscriptional activator of cyn operon; autorepressor
*NC_0009132,151,5810GT40.0% 77.0 / 20.5 56D133Y (GAT→TAT) yegJuncharacterized protein
*NC_000913599,5760TG39.6% 86.8 / 10.1 56V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
*NC_0009133,556,2070TC38.6% 91.6 / 13.7 57S215G (AGT→GGT) rtcARNA 3'‑terminal phosphate cyclase
*NC_0009133,508,6620TA38.3% 99.1 / 18.0 61E384D (GAA→GATyhfWphosphopentomutase‑related metalloenzyme superfamily protein
*NC_000913802,1180AC38.2% 77.5 / 12.9 56T78P (ACC→CCC) ybhIputative DASS family tricarboxylate or dicarboxylate transporter
*NC_0009132,421,4370TC37.7% 72.1 / 11.7 53I38T (ATC→ACC) folXD‑erythro‑7,8‑dihydroneopterin triphosphate 2'‑epimerase and dihydroneopterin aldolase
*NC_0009134,555,1090TA37.7% 79.3 / 15.1 54N178K (AAT→AAAuxuRfructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 237681168 (1.020)3 (0.050) 3/290 12.6 4.3% coding (9/858 nt) menB dihydroxynaphthoic acid synthetase
?NC_000913 = 2376825 67 (1.040)intergenic (‑6/+9) menB/menH dihydroxynaphthoic acid synthetase/2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase