breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 91 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3610C.76.9% 71.9 / 15.9 26pseudogene (2/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
*NC_000913281,0890AC71.4% ‑1.5 / 12.3 21Y299D (TAT→GAT) yagACP4‑6 prophage; putative DNA‑binding transcriptional regulator
*NC_0009132,151,6890GT64.7% 12.8 / 6.5 35intergenic (+43/+22)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
*NC_0009132,228,2220TG62.5% 0.1 / 11.6 16pseudogene (388/1287 nt)mdtQpseudogene; putative channel/filament proteins
*NC_0009132,386,8620GA62.5% 0.0 / 13.2 17intergenic (+31/‑72)yfbL/yfbMputative M28A family peptidase/DUF1877 family protein
*NC_0009134,223,6200TG61.1% 1.3 / 11.5 18A3A (GCA→GCCiclRtranscriptional repressor
*NC_0009133,575,9470AC60.0% 6.9 / 13.5 15V372G (GTT→GGT) gntUgluconate transporter, low affinity GNT 1 system
*NC_0009133,813,0720AC58.8% 5.0 / 14.7 17T114T (ACA→ACCdfpfused 4'‑phosphopantothenoylcysteine decarboxylase/phosphopantothenoylcysteine synthetase, FMN‑binding
*NC_0009132,337,7230AC58.3% 3.6 / 11.3 13G566G (GGT→GGGgyrADNA gyrase (type II topoisomerase), subunit A
*NC_0009134,223,5970CT57.9% ‑3.5 / 10.7 19R11K (AGA→AAA) iclRtranscriptional repressor
*NC_0009134,223,6340TG56.2% 10.7 / 19.2 16intergenic (‑6/‑194)iclR/metHtranscriptional repressor/homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
*NC_0009134,111,7470GT56.0% 10.9 / 36.1 25N156K (AAC→AAAyiiQDUF1454 family putative periplasmic protein
*NC_0009131,072,7310TG55.6% 13.9 / 20.7 18T45P (ACC→CCC) rutBureidoacrylate amidohydrolase
*NC_0009134,223,6140AG55.6% 8.4 / 21.7 18I5I (ATT→ATCiclRtranscriptional repressor
*NC_000913345,3280CA55.0% 23.0 / 66.4 40intergenic (+337/‑76)yahL/yahMuncharacterized protein/uncharacterized protein
*NC_000913354,3180TG55.0% 10.1 / 25.9 20V538G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NC_0009132,480,3710TG55.0% 15.8 / 26.1 20G390G (GGT→GGGdsdAD‑serine dehydratase
*NC_000913993,9130GC54.5% 1.7 / 15.1 12G44G (GGC→GGGssuBaliphatic sulfonate ABC transporter ATPase
*NC_0009131,755,8710TA54.5% 0.6 / 27.5 23G58G (GGT→GGApykFpyruvate kinase I
*NC_0009131,973,5350TC54.5% 4.7 / 12.7 11D597G (GAT→GGT) cheAfused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =21 (0.800)3 (0.130) 3/266 2.7 14.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 17 (0.730)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 3497094 =29 (1.110)3 (0.120) 3/274 2.8 9.7% coding (92/807 nt) nirC nitrite transporter
?NC_000913 3497106 = 29 (1.200)coding (104/807 nt) nirC nitrite transporter
* ? NC_000913 1647054 =22 (0.840)3 (0.120) 3/276 2.8 14.2% intergenic (+317/+120) flxA/intK Qin prophage; uncharacterized protein/pseudogene, integrase fragment, Qin prophage;Phage or Prophage Related
?NC_000913 1647120 = 16 (0.660)intergenic (+383/+54) flxA/intK Qin prophage; uncharacterized protein/pseudogene, integrase fragment, Qin prophage;Phage or Prophage Related
* ? NC_000913 = 448189815 (0.570)3 (0.120) 3/282 2.9 17.5% coding (1940/2856 nt) valS valyl‑tRNA synthetase
?NC_000913 = 4481970 14 (0.560)coding (1868/2856 nt) valS valyl‑tRNA synthetase
* ? NC_000913 3566202 =31 (1.180)3 (0.120) 3/282 2.9 9.0% coding (380/2448 nt) glgP glycogen phosphorylase
?NC_000913 3566229 = 31 (1.250)coding (353/2448 nt) glgP glycogen phosphorylase
* ? NC_000913 = 334029630 (1.140)3 (0.120) 3/280 2.9 9.3% coding (22/978 nt) yrbG putative calcium/sodium:proton antiporter
?NC_000913 = 3340323 30 (1.220)coding (49/978 nt) yrbG putative calcium/sodium:proton antiporter