breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 36,231 T→C intergenic (‑69/+40) caiD ← / ← caiC carnitinyl‑CoA dehydratase/putative crotonobetaine/carnitine‑CoA ligase
RA 162,973 G→A C290Y (TGT→TAT)  hrpB → putative ATP‑dependent helicase
MC JC 190,553 Δ14 bp coding (680‑693/726 nt) rpsB → 30S ribosomal subunit protein S2
RA 216,568 C→T A146T (GCC→ACC)  yaeF ← putative lipoprotein
RA 228,818 C→A noncoding (63/120 nt) rrfH → 5S ribosomal RNA of rrnH operon
RA 236,541 C→T R159* (CGA→TGA)  dnaQ → DNA polymerase III epsilon subunit
MC JC 257,908 Δ776 bp [crl] [crl]
RA 428,188 G→T R181L (CGC→CTC)  secD → SecYEG protein translocase auxillary subunit
RA 497,139 (T)5→6 intergenic (+145/‑36) htpG → / → adk protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase
RA 520,993 T→G V193G (GTC→GGC)  ybbP → putative ABC transporter permease
RA 700,805 A→T C264S (TGC→AGC)  nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
RA 720,946 C→T intergenic (+106/+110) ybfK → / ← kdpE uncharacterized protein/response regulator in two‑component regulatory system with KdpD
RA 772,209 C→A T251N (ACC→AAC)  cydA → cytochrome d terminal oxidase, subunit I
RA 882,722 G→A R5C (CGC→TGC)  deoR ← deoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx
RA 894,155 T→G D124E (GAT→GAG potF → putrescine ABC transporter periplasmic binding protein
RA 1,100,233 G→A intergenic (+39/‑63) ycdY → / → ycdZ redox enzyme maturation protein (REMP) chaperone for YcdX/DUF1097 family inner membrane protein
RA 1,143,125 C→T D415N (GAC→AAC)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
RA 1,197,739 A→G S167P (TCC→CCC)  ymfE ← e14 prophage; putative inner membrane protein
RA 1,292,944 T→C intergenic (‑22/‑583) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,401,844 A→G I471T (ATC→ACC)  abgB ← p‑aminobenzoyl‑glutamate hydrolase, B subunit
RA 1,434,459 C→T intergenic (‑235/+499) ynaE ← / ← ttcC cold shock protein, Rac prophage/pseudogene, prophage Rac integration site ttcA duplication;Phage or Prophage Related
RA 1,501,912 T→A I117I (ATT→ATA tehB → tellurite, selenium methyltransferase, SAM‑dependent; tellurite, selenium resistance protein
RA 1,530,076 (A)7→8 coding (155/483 nt) ydcD → putative immunity protein for RhsE
RA 1,580,094 C→T D233N (GAT→AAT)  ydeM ← putative YdeN‑specific sulfatase‑maturating enzyme
RA 1,665,195 (T)7→8 intergenic (+75/‑120) dmsD → / → clcB twin‑argninine leader‑binding protein for DmsA and TorA/H(+)/Cl(‑) exchange transporter
RA 1,681,932 T→C P33P (CCA→CCG ydgC ← GlpM family inner membrane protein
RA 1,712,306 (T)7→8 intergenic (+148/‑463) nth → / → dtpA DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
RA 1,712,518 (C)6→5 intergenic (+360/‑251) nth → / → dtpA DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
RA 1,728,444 C→A T33N (ACC→AAC)  rnt → RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair
RA 1,827,031 T→A D410V (GAT→GTT)  astB ← succinylarginine dihydrolase
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 1,983,761 C→T E437K (GAA→AAA)  araG ← L‑arabinose ABC transporter ATPase
RA 2,071,321 A→G intergenic (+110/‑218) yeeP → / → flu pseudogene, CP4‑44 prophage; 50S ribosome‑binding GTPase family;Phage or Prophage Related; putative histone/CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,191,432 C→T G72S (GGC→AGC)  yehC ← putative periplasmic pilin chaperone
RA 2,210,811 C→T pseudogene (1712/2001 nt) yehQ → pseudogene
RA 2,278,298 C→T intergenic (‑61/+272) yejG ← / ← bcr uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
RA 2,436,937 A→T D305E (GAT→GAA pdxB ← erythronate‑4‑phosphate dehydrogenase
RA 2,469,656 (T)7→8 coding (526/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,521,967 C→A A171S (GCG→TCG)  xapR ← transcriptional activator of xapAB
RA 2,563,518 (A)5→6 intergenic (+401/‑74) yffP → / → yffQ CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
RA 2,564,525 G→A M1M (ATG→ATA)  † yffS → CPZ‑55 prophage; uncharacterized protein
RA 2,600,288 C→T S128L (TCG→TTG)  gcvR → transcriptional repressor, regulatory protein accessory to GcvA
RA 2,810,756 G→A intergenic (+77/‑14) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
RA 2,852,356 T→A L74Q (CTG→CAG)  hypD → hydrogenase maturation protein
RA 2,887,352 T→A intergenic (‑133/+226) ygcB ← / ← cysH Cascade complex anti‑viral R‑loop helicase‑annealase Cas3/phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent
RA 3,452,057 A→T I77N (ATC→AAC)  rplD ← 50S ribosomal subunit protein L4
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,622,796 T→G L1202R (CTG→CGG)  rhsB → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 3,648,042 T→C intergenic (‑254/‑486) dinQ ← / → arsR UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/arsenical resistance operon transcriptional repressor; autorepressor
RA 3,853,909 A→G intergenic (+267/‑13) tisB → / → emrD toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein
RA 3,910,662 A→T D288E (GAT→GAA pstS ← phosphate ABC transporter periplasmic binding protein
RA 3,950,468 G→T intergenic (+48/‑39) ilvL → / → ilvX ilvG operon leader peptide/uncharacterized protein
RA 3,971,690 A→C Q144P (CAG→CCG)  wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
RA 4,168,540 T→A intergenic (+93/‑101) gltT → / → rrlB tRNA‑Glu/23S ribosomal RNA of rrnB operon
RA 4,235,689 +A intergenic (+282/‑217) pgi → / → yjbE glucosephosphate isomerase/extracellular polysaccharide production threonine‑rich protein
MC JC 4,242,942 Δ66 bp coding (510‑575/891 nt) malG ← maltose transporter subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,490,055 T→G E3D (GAA→GAC yjgR ← DUF853 family protein with NTPase fold
RA 4,615,859 T→G T403P (ACC→CCC)  yjjI ← DUF3029 family protein, putative glycine radical enzyme
RA 4,631,700 T→C L323P (CTT→CCT)  slt → lytic murein transglycosylase, soluble

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423765–3424234 3424548–3424238 5–784 47 [44] [43] 46 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)112 (1.030) 63/290 0.2 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein