breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA CP000731 81 N→G 100% intergenic (–/‑151)  / → repA –/replication protein A
JC CP000731 6,552 +28 bp 100% intergenic (+1069/‑419) cadX → / → USA300HOU_pUSA300HOUMR0010 cadmium efflux regulator/MarR family transcriptional regulator
RA CP000731 25,663 Δ1 bp 100% coding (316/333 nt) USA300HOU_pUSA300HOUMR0031 → hypothetical protein
RA USA300TCH1516_ALE 512,643 A→G 48.7% intergenic (+711/‑6052) recR → / → speA_2 Recombination protein RecR/Arginine decarboxylase
RA USA300TCH1516_ALE 748,768 A→G 19.5% I255V (ATT→GTT)  oxyR → Hydrogen peroxide‑inducible genes activator
RA USA300TCH1516_ALE 949,407 G→T 12.5% intergenic (+194/‑222) USA300HOU_RS04730 → / → yugI_2 Putative peptidyl‑prolyl cis‑trans isomerase/General stress protein 13
RA USA300TCH1516_ALE 1,201,454 T→C 18.2% V144A (GTT→GCT)  ileS → Isoleucine‑‑tRNA ligase
RA USA300TCH1516_ALE 1,416,458 A→T 100% I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD
RA USA300TCH1516_ALE 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_RS07375 → hypothetical protein
RA USA300TCH1516_ALE 1,576,651 A→G 23.6% F289S (TTC→TCC)  USA300HOU_RS07705 ← Glycyl‑glycine endopeptidase ALE‑1
RA USA300TCH1516_ALE 1,646,189 G→C 100% A155G (GCA→GGA)  ypdF ← Aminopeptidase YpdF
RA USA300TCH1516_ALE 1,884,522 C→T 11.1% A233T (GCC→ACC)  ebh_2 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_RS10125 ← Putative multidrug export ATP‑binding/permease protein
RA USA300TCH1516_ALE 2,279,705 G→A 100% A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 2,366,219 G→A 11.7% A46V (GCA→GTA)  rplN ← 50S ribosomal protein L14
RA USA300TCH1516_ALE 2,484,799 G→A 10.2% A295V (GCG→GTG)  mqo1 ← putative malate:quinone oxidoreductase 1
RA USA300TCH1516_ALE 2,504,803 T→C 12.9% intergenic (+107/+297) USA300HOU_RS12890 → / ← gltT hypothetical protein/Proton/sodium‑glutamate symport protein
RA USA300TCH1516_ALE 2,532,298 A→T 100% T15T (ACA→ACT femA_3 → Aminoacyltransferase FemA
RA USA300TCH1516_ALE 2,596,868 A→T 9.7% Y212N (TAC→AAC)  nikA_2 ← Nickel‑binding periplasmic protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 512620 =18 (0.830)9 (0.600)
+40 bp
9/180 NT 42.0% intergenic (+688/‑6075) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
?USA300TCH1516_ALE 1998278 = NA (NA)intergenic (‑6/+3329) USA300TCH1516_01882/USA300TCH1516_01883 tRNA‑Val/tRNA‑Ala