Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F13 I1 R1 57 60.3 2567246 98.7% 2533871 146.1

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NC_002947_CJ‑RC/4348664‑4348961
                                                                                                                                                    |                                                                                                                                                     
GATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATC                                                                                                                                                     >  NB501016:282:H2V7GAFX2:4:21511:3289:9414/1‑151 (MQ=0)
        ATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCC                                                                                                                                             <  NB501016:282:H2V7GAFX2:1:21310:3886:7975/151‑1 (MQ=0)
            CAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTC                                                                                                                                         >  NB501016:282:H2V7GAFX2:3:21411:4759:15075/1‑151 (MQ=0)
                         GGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGC                                                                                                                            <  NB501016:282:H2V7GAFX2:1:11304:11282:5326/151‑1 (MQ=0)
                         GGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAG                                                                                                                             <  NB501016:282:H2V7GAFX2:3:11508:9816:2777/150‑1 (MQ=0)
                         GGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTT                                                                                                                                           >  NB501016:282:H2V7GAFX2:2:11106:25053:4671/1‑136 (MQ=0)
                          GGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGC                                                                                                                            >  NB501016:282:H2V7GAFX2:3:11611:24731:15451/1‑150 (MQ=0)
                            AAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACA                                                                                                                         <  NB501016:282:H2V7GAFX2:1:11111:22510:2084/151‑1 (MQ=0)
                                TTCTGCATTACGGGTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTG                                                                                                                      <  NB501016:282:H2V7GAFX2:4:21509:26367:13561/150‑1 (MQ=0)
                                 TCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGG                                                                                                                     <  NB501016:282:H2V7GAFX2:2:11309:25791:18575/150‑1 (MQ=0)
                                      ATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCG                                                                                                               <  NB501016:282:H2V7GAFX2:3:11607:9476:4706/151‑1 (MQ=17)
                                          CGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCATTGGTGGCGCGGTTAACCGCCACTTACAACGGCTTCGGCTGACTTCTCCACGCTCGTCCCATCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                            >  NB501016:282:H2V7GAFX2:3:11604:11913:13033/1‑150 (MQ=19)
                                           GGTTACCAACGAGACGGTGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                            >  NB501016:282:H2V7GAFX2:3:21502:23806:15826/1‑149 (MQ=20)
                                                   ACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCA                                                                                                  <  NB501016:282:H2V7GAFX2:4:11512:8120:18546/151‑1 (MQ=22)
                                                      AGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTATCACAGTGGCAAGCGTACAGATCTCCCAGGG                                                                                               <  NB501016:282:H2V7GAFX2:2:11109:3369:14914/151‑1 (MQ=20)
                                                      AGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGG                                                                                               <  NB501016:282:H2V7GAFX2:1:21103:11667:11898/151‑1 (MQ=22)
                                                               CGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCG                                                                                      >  NB501016:282:H2V7GAFX2:3:21407:8543:12440/1‑151 (MQ=22)
                                                               CGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACCGCTTCGGCTGACTTCTGCACGCTCGTCCCGACGCCTTTCGACGCGCGGTAGCACAGTCGCAAGCGTACAGATCTCCCAGGGTAATTCGCG                                                                                      >  NB501016:282:H2V7GAFX2:4:21505:1506:13252/1‑151 (MQ=19)
                                                                 CCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCG                                                                                    <  NB501016:282:H2V7GAFX2:3:11504:23606:10053/151‑1 (MQ=21)
                                                                 CCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCG                                                                                    >  NB501016:282:H2V7GAFX2:3:11601:19363:13118/1‑151 (MQ=22)
                                                                           GCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTAC                                                                          >  NB501016:282:H2V7GAFX2:4:21403:1365:7266/1‑151 (MQ=21)
                                                                            CATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCT                                                                         >  NB501016:282:H2V7GAFX2:2:21305:19114:6245/1‑151 (MQ=21)
                                                                             ATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTTCGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTT                                                                        <  NB501016:282:H2V7GAFX2:1:11211:23215:3882/151‑1 (MQ=20)
                                                                               AACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTA                                                                       <  NB501016:282:H2V7GAFX2:1:11304:25456:17963/150‑1 (MQ=22)
                                                                               AACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTA                                                                       <  NB501016:282:H2V7GAFX2:4:21603:14649:6584/150‑1 (MQ=22)
                                                                                ACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATG                                                                     <  NB501016:282:H2V7GAFX2:1:11307:12565:20257/151‑1 (MQ=21)
                                                                                ACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATG                                                                     <  NB501016:282:H2V7GAFX2:4:11603:15714:4767/151‑1 (MQ=21)
                                                                                ACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATG                                                                     <  NB501016:282:H2V7GAFX2:4:11503:9026:1727/151‑1 (MQ=22)
                                                                                 CCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                            <  NB501016:282:H2V7GAFX2:3:11604:18651:12415/111‑1 (MQ=24)
                                                                                    GTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCCACCTTCCTGCTTATGCC                                                                   <  NB501016:282:H2V7GAFX2:2:11108:24141:3764/149‑1 (MQ=20)
                                                                                       CGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCG                                                              <  NB501016:282:H2V7GAFX2:3:21409:17565:17177/151‑1 (MQ=22)
                                                                                         GCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGG                                                             >  NB501016:282:H2V7GAFX2:3:21607:12579:1739/1‑150 (MQ=21)
                                                                                          CCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGAT                                                           >  NB501016:282:H2V7GAFX2:3:21606:2433:16596/1‑151 (MQ=21)
                                                                                           CCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCG                                                              >  NB501016:282:H2V7GAFX2:3:21509:22632:5386/1‑147 (MQ=22)
                                                                                               GGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCGCGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTTTCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACG                                                      <  NB501016:282:H2V7GAFX2:1:21305:11406:12914/151‑1 (MQ=20)
                                                                                                  GGCGCGGTTAACCGCCACTTACTACGGCTTCGTCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTC                                                    <  NB501016:282:H2V7GAFX2:4:21612:6221:11159/150‑1 (MQ=20)
                                                                                                          TAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTA                                            <  NB501016:282:H2V7GAFX2:1:21206:11089:11822/150‑1 (MQ=21)
                                                                                                            ACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCG                                         <  NB501016:282:H2V7GAFX2:3:11511:4301:16312/151‑1 (MQ=21)
                                                                                                                  ACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAG                                   <  NB501016:282:H2V7GAFX2:2:11112:14033:11585/151‑1 (MQ=21)
                                                                                                                        TACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGG                             >  NB501016:282:H2V7GAFX2:1:11107:2687:11880/1‑151 (MQ=22)
                                                                                                                         ACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGAGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGG                            >  NB501016:282:H2V7GAFX2:4:11602:6202:10151/1‑151 (MQ=20)
                                                                                                                          CGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGGCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGC                           <  NB501016:282:H2V7GAFX2:1:11211:10148:13208/151‑1 (MQ=21)
                                                                                                                            GCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTT                         >  NB501016:282:H2V7GAFX2:3:21505:17715:5335/1‑151 (MQ=21)
                                                                                                                            GCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTT                         >  NB501016:282:H2V7GAFX2:4:11503:7686:10392/1‑151 (MQ=22)
                                                                                                                                 GGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCG                    >  NB501016:282:H2V7GAFX2:2:11106:2597:18733/1‑151 (MQ=22)
                                                                                                                                 GGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCG                    <  NB501016:282:H2V7GAFX2:2:11112:21713:3277/151‑1 (MQ=22)
                                                                                                                                     GACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATT                 <  NB501016:282:H2V7GAFX2:2:11312:1782:6804/150‑1 (MQ=22)
                                                                                                                                      ACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTG               <  NB501016:282:H2V7GAFX2:1:11305:26287:17737/151‑1 (MQ=22)
                                                                                                                                       CTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGT              >  NB501016:282:H2V7GAFX2:3:21508:8560:5868/1‑151 (MQ=21)
                                                                                                                                       CTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGT              >  NB501016:282:H2V7GAFX2:3:11510:1363:15209/1‑151 (MQ=21)
                                                                                                                                                 CTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAC    >  NB501016:282:H2V7GAFX2:4:11508:10118:12084/1‑151 (MQ=21)
                                                                                                                                                   CGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NB501016:282:H2V7GAFX2:3:11502:6859:2204/1‑151 (MQ=21)
                                                                                                                                                   CATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTAATGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NB501016:282:H2V7GAFX2:3:21606:9034:8235/1‑151 (MQ=21)
                                                                                                                                                    |                                                                                                                                                     
GATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NC_002947_CJ‑RC/4348664‑4348961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑