Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 59 79.3 3391134 98.6% 3343658 146.3

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NC_002947_CJ‑RC/4348665‑4348961
                                                                                                                                                   |                                                                                                                                                     
ATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCC                                                                                                                                                    >  NB501016:282:H2V7GAFX2:1:21110:13642:12425/1‑151 (MQ=0)
     GGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCG                                                                                                                                               >  NB501016:282:H2V7GAFX2:4:21602:16376:1772/1‑151 (MQ=0)
             GTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCG                                                                                                                                        >  NB501016:282:H2V7GAFX2:3:11403:4699:2768/1‑150 (MQ=0)
               TTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGAC                                                                                                                                      <  NB501016:282:H2V7GAFX2:1:21104:22168:4803/150‑1 (MQ=0)
                               TTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGG                                                                                                                     >  NB501016:282:H2V7GAFX2:1:11311:7315:3311/1‑151 (MQ=0)
                               TTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTG                                                                                                                      <  NB501016:282:H2V7GAFX2:1:11309:21862:16871/150‑1 (MQ=0)
                               TTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTG                                                                                                                      <  NB501016:282:H2V7GAFX2:2:21102:25937:15877/150‑1 (MQ=0)
                                TCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC                                                                                                                    >  NB501016:282:H2V7GAFX2:4:21603:15789:5722/1‑151 (MQ=1)
                                   GCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC                                                                                                                    <  NB501016:282:H2V7GAFX2:1:11311:24693:7603/148‑1 (MQ=2)
                                   GCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAG                                                                                                                 <  NB501016:282:H2V7GAFX2:4:11609:2324:1813/151‑1 (MQ=4)
                                             TACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                            >  NB501016:282:H2V7GAFX2:3:21507:4787:16838/1‑146 (MQ=21)
                                              ACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAATGGCAAGCGTACAGATC                                                                                                       <  NB501016:282:H2V7GAFX2:3:11603:3105:17737/150‑1 (MQ=0)
                                               CCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTC                                                                                                     >  NB501016:282:H2V7GAFX2:1:21208:23510:12227/1‑151 (MQ=21)
                                                CAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCC                                                                                                    >  NB501016:282:H2V7GAFX2:1:21109:2585:19078/1‑151 (MQ=22)
                                                 AACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCC                                                                                                   >  NB501016:282:H2V7GAFX2:3:21410:3636:11683/1‑151 (MQ=21)
                                                 AACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCCTACAGATCTCCC                                                                                                   >  NB501016:282:H2V7GAFX2:2:21109:19881:20056/1‑151 (MQ=20)
                                                  ACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCA                                                                                                  <  NB501016:282:H2V7GAFX2:2:21207:19594:4385/151‑1 (MQ=21)
                                                   CGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCC                                                                                                   >  NB501016:282:H2V7GAFX2:4:11501:5504:17729/1‑149 (MQ=22)
                                                   CGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCC                                                                                                   >  NB501016:282:H2V7GAFX2:1:21202:14876:12455/1‑149 (MQ=22)
                                                       ACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGTTA                                                                                             <  NB501016:282:H2V7GAFX2:2:11209:1365:5726/151‑1 (MQ=21)
                                                                  TACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGAC                                                                                  >  NB501016:282:H2V7GAFX2:4:11507:2059:6327/1‑151 (MQ=21)
                                                                      GGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTC                                                                              >  NB501016:282:H2V7GAFX2:2:21109:14509:18548/1‑151 (MQ=21)
                                                                      GGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTC                                                                              >  NB501016:282:H2V7GAFX2:4:21611:3534:4220/1‑151 (MQ=21)
                                                                      GGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTC                                                                              >  NB501016:282:H2V7GAFX2:4:11404:24436:8123/1‑151 (MQ=22)
                                                                        CGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCT                                                                            >  NB501016:282:H2V7GAFX2:4:21603:9258:3628/1‑151 (MQ=21)
                                                                         GGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTG                                                                           >  NB501016:282:H2V7GAFX2:1:21105:2692:5649/1‑151 (MQ=22)
                                                                           CATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGTGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCT                                                                         >  NB501016:282:H2V7GAFX2:3:21406:9046:11532/1‑151 (MQ=20)
                                                                           CATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCT                                                                         <  NB501016:282:H2V7GAFX2:3:11511:13171:8480/151‑1 (MQ=21)
                                                                           CATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCT                                                                         >  NB501016:282:H2V7GAFX2:4:11501:20845:3587/1‑151 (MQ=22)
                                                                              AACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTAT                                                                      <  NB501016:282:H2V7GAFX2:4:11503:6324:16863/151‑1 (MQ=22)
                                                                               ACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCAGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATG                                                                     >  NB501016:282:H2V7GAFX2:3:11604:19278:8058/1‑151 (MQ=20)
                                                                               ACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATG                                                                     >  NB501016:282:H2V7GAFX2:4:21610:8502:4952/1‑151 (MQ=22)
                                                                               ACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATG                                                                     >  NB501016:282:H2V7GAFX2:3:11510:13598:12189/1‑151 (MQ=22)
                                                                               ACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATG                                                                     >  NB501016:282:H2V7GAFX2:2:11106:16370:13641/1‑151 (MQ=22)
                                                                                   GTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCT                                                                  <  NB501016:282:H2V7GAFX2:3:21408:8840:18014/150‑1 (MQ=21)
                                                                                   GTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCC                                                                   <  NB501016:282:H2V7GAFX2:2:11101:4426:15045/149‑1 (MQ=21)
                                                                                      CGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTC                                                               <  NB501016:282:H2V7GAFX2:3:21502:9363:15752/150‑1 (MQ=22)
                                                                                       GGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGG                                                             >  NB501016:282:H2V7GAFX2:4:21509:6972:7991/1‑151 (MQ=22)
                                                                                         CCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGAT                                                           >  NB501016:282:H2V7GAFX2:4:11603:7336:5206/1‑151 (MQ=21)
                                                                                         CCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGAT                                                           >  NB501016:282:H2V7GAFX2:3:11501:10486:7247/1‑151 (MQ=22)
                                                                                          CCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATC                                                          >  NB501016:282:H2V7GAFX2:4:11403:24143:4301/1‑151 (MQ=21)
                                                                                              GGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACG                                                      >  NB501016:282:H2V7GAFX2:2:11303:17794:3755/1‑151 (MQ=21)
                                                                                              GGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACG                                                      <  NB501016:282:H2V7GAFX2:3:21505:19963:18625/151‑1 (MQ=21)
                                                                                              GGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACG                                                      <  NB501016:282:H2V7GAFX2:1:11110:6307:8979/151‑1 (MQ=22)
                                                                                                   CGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCAC                                                  >  NB501016:282:H2V7GAFX2:2:21111:3853:18701/1‑150 (MQ=21)
                                                                                                   CGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCAC                                                  >  NB501016:282:H2V7GAFX2:4:11506:19492:9481/1‑150 (MQ=21)
                                                                                                    GCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAG                                                <  NB501016:282:H2V7GAFX2:4:11411:8316:2059/151‑1 (MQ=22)
                                                                                                                 ACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAG                                   <  NB501016:282:H2V7GAFX2:3:21507:21869:17468/151‑1 (MQ=21)
                                                                                                                  CTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTACCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGT                                  >  NB501016:282:H2V7GAFX2:3:21404:10049:9588/1‑151 (MQ=20)
                                                                                                                     ACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATT                               >  NB501016:282:H2V7GAFX2:3:21410:8586:17396/1‑151 (MQ=21)
                                                                                                                      CTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTG                              >  NB501016:282:H2V7GAFX2:4:21502:2082:20110/1‑151 (MQ=21)
                                                                                                                      CTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCG                                         >  NB501016:282:H2V7GAFX2:3:11605:15741:1600/1‑140 (MQ=22)
                                                                                                                        ACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGG                            >  NB501016:282:H2V7GAFX2:1:21211:1876:8661/1‑151 (MQ=21)
                                                                                                                        ACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTG                              <  NB501016:282:H2V7GAFX2:4:21505:9596:1842/149‑1 (MQ=21)
                                                                                                                         CGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGC                           <  NB501016:282:H2V7GAFX2:1:21301:4189:12179/151‑1 (MQ=22)
                                                                                                                          GGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCT                          >  NB501016:282:H2V7GAFX2:1:21108:24589:13454/1‑151 (MQ=22)
                                                                                                                          GGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCT                          >  NB501016:282:H2V7GAFX2:2:21201:13693:11535/1‑151 (MQ=22)
                                                                                                                              TCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTG                       <  NB501016:282:H2V7GAFX2:2:21111:13991:4412/150‑1 (MQ=22)
                                                                                                                               CGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGC                     <  NB501016:282:H2V7GAFX2:2:21210:15415:2267/151‑1 (MQ=21)
                                                                                                                                GGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCG                    >  NB501016:282:H2V7GAFX2:3:21412:22381:15594/1‑151 (MQ=22)
                                                                                                                                      CTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGT              >  NB501016:282:H2V7GAFX2:1:21307:17429:13032/1‑151 (MQ=21)
                                                                                                                                        TCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTG             <  NB501016:282:H2V7GAFX2:3:11406:3653:12259/150‑1 (MQ=21)
                                                                                                                                        TCTGCACGCTCGT‑CCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTG             <  NB501016:282:H2V7GAFX2:4:21505:6509:10753/149‑1 (MQ=20)
                                                                                                                                        TCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTG             <  NB501016:282:H2V7GAFX2:1:21101:18967:10573/150‑1 (MQ=22)
                                                                                                                                         CTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCC           >  NB501016:282:H2V7GAFX2:4:11511:20166:18407/1‑151 (MQ=21)
                                                                                                                                            CACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACC        <  NB501016:282:H2V7GAFX2:3:11408:10331:17131/151‑1 (MQ=21)
                                                                                                                                            CACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACC        >  NB501016:282:H2V7GAFX2:4:21511:15896:10057/1‑151 (MQ=21)
                                                                                                                                            CACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGA                                                                                                         <  NB501016:282:H2V7GAFX2:3:11409:21662:6259/54‑1 (MQ=0)
                                                                                                                                              CGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTC      >  NB501016:282:H2V7GAFX2:3:11508:9745:3824/1‑151 (MQ=21)
                                                                                                                                                CTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAC    >  NB501016:282:H2V7GAFX2:4:21402:5279:5041/1‑151 (MQ=21)
                                                                                                                                                CTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAC    >  NB501016:282:H2V7GAFX2:2:21206:2512:16231/1‑151 (MQ=22)
                                                                                                                                                  CATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NB501016:282:H2V7GAFX2:1:11112:15213:13019/1‑151 (MQ=22)
                                                                                                                                                   |                                                                                                                                                     
ATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NC_002947_CJ‑RC/4348665‑4348961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑