Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F53 I1 R1 62 81.2 3465250 98.6% 3416736 145.3

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCCC  >  NC_002947_CJ‑RC/4348664‑4348962
                                                                                                                                                    |                                                                                                                                                      
GATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTC                                                                                                                                                      <  NB501016:282:H2V7GAFX2:4:21611:25411:8258/151‑1 (MQ=0)
        ATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCC                                                                                                                                              >  NB501016:282:H2V7GAFX2:4:21508:8778:12565/1‑151 (MQ=0)
          GACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTT                                                                                                                                            >  NB501016:282:H2V7GAFX2:2:21303:16434:1885/1‑151 (MQ=0)
          GACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCT                                                                                                                                             <  NB501016:282:H2V7GAFX2:2:11109:2334:9776/150‑1 (MQ=0)
            CAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTC                                                                                                                                          <  NB501016:282:H2V7GAFX2:3:21404:21182:18411/151‑1 (MQ=0)
                TTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGAC                                                                                                                                       <  NB501016:282:H2V7GAFX2:1:11304:26473:1859/150‑1 (MQ=0)
                   CGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGC                                                                                                                                   >  NB501016:282:H2V7GAFX2:1:11208:16595:8979/1‑151 (MQ=0)
                    GCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCG                                                                                                                                  <  NB501016:282:H2V7GAFX2:2:11308:22876:12665/151‑1 (MQ=0)
                       GTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGG                                                                                                                                 <  NB501016:282:H2V7GAFX2:2:11109:25400:14272/149‑1 (MQ=0)
                         GGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGC                                                                                                                             <  NB501016:282:H2V7GAFX2:1:21212:25588:8338/151‑1 (MQ=0)
                         GGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAG                                                                                                                              <  NB501016:282:H2V7GAFX2:3:21506:16778:3821/150‑1 (MQ=0)
                         GGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAG                                                                                                                              <  NB501016:282:H2V7GAFX2:3:11502:16562:7435/150‑1 (MQ=0)
                          GGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCA                                                                                                                            <  NB501016:282:H2V7GAFX2:1:21201:21377:18286/151‑1 (MQ=0)
                            AAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACA                                                                                                                          <  NB501016:282:H2V7GAFX2:1:11112:26358:13271/151‑1 (MQ=0)
                              ATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGT                                                                                                                        <  NB501016:282:H2V7GAFX2:1:11203:22442:11457/151‑1 (MQ=0)
                                TTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTG                                                                                                                       <  NB501016:282:H2V7GAFX2:4:21603:13586:19108/150‑1 (MQ=0)
                                 TCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGG                                                                                                                      <  NB501016:282:H2V7GAFX2:1:21102:10403:17524/150‑1 (MQ=0)
                                  CTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC                                                                                                                     >  NB501016:282:H2V7GAFX2:3:21412:18177:9016/1‑150 (MQ=2)
                                   TGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCA                                                                                                                    >  NB501016:282:H2V7GAFX2:3:21604:17186:11850/1‑150 (MQ=2)
                                         ACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                             <  NB501016:282:H2V7GAFX2:4:11501:26328:3042/151‑1 (MQ=22)
                                         ACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                             <  NB501016:282:H2V7GAFX2:3:11404:3107:8477/151‑1 (MQ=22)
                                               ACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATC                                                                                                        <  NB501016:282:H2V7GAFX2:2:21208:14987:1625/150‑1 (MQ=21)
                                                    CGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAG                                                                                                  <  NB501016:282:H2V7GAFX2:2:11105:22793:7494/151‑1 (MQ=21)
                                                    CGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCC                                                                                                    >  NB501016:282:H2V7GAFX2:2:21204:8376:2471/1‑149 (MQ=21)
                                                      AGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGG                                                                                                <  NB501016:282:H2V7GAFX2:4:21411:17904:20313/151‑1 (MQ=21)
                                                       GACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGT                                                                                               <  NB501016:282:H2V7GAFX2:4:11608:7447:15939/151‑1 (MQ=22)
                                                        ACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTA                                                                                              <  NB501016:282:H2V7GAFX2:4:21601:1881:10787/151‑1 (MQ=21)
                                                          GGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAAT                                                                                            >  NB501016:282:H2V7GAFX2:3:11405:12394:19476/1‑151 (MQ=21)
                                                             GACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCG                                                                                         >  NB501016:282:H2V7GAFX2:3:11603:22420:16583/1‑151 (MQ=22)
                                                               CGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCG                                                                                       >  NB501016:282:H2V7GAFX2:1:11208:12823:3957/1‑151 (MQ=21)
                                                               CGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCG                                                                                       <  NB501016:282:H2V7GAFX2:1:11307:22498:4895/151‑1 (MQ=21)
                                                                  CTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGC                                                                                    >  NB501016:282:H2V7GAFX2:1:21205:12503:2502/1‑151 (MQ=21)
                                                                     CTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCT                                                                                 >  NB501016:282:H2V7GAFX2:1:21201:9442:11050/1‑151 (MQ=21)
                                                                       GGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTT                                                                                <  NB501016:282:H2V7GAFX2:1:21307:23460:6153/150‑1 (MQ=21)
                                                                        GCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCC                                                                              <  NB501016:282:H2V7GAFX2:3:11512:6934:9956/151‑1 (MQ=21)
                                                                            CATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCT                                                                          >  NB501016:282:H2V7GAFX2:2:11301:3856:14440/1‑151 (MQ=22)
                                                                            CATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCACGCGACCTTCCTGCT                                                                          >  NB501016:282:H2V7GAFX2:1:11305:7286:15347/1‑151 (MQ=21)
                                                                             ATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTT                                                                         <  NB501016:282:H2V7GAFX2:2:21101:18394:14773/151‑1 (MQ=22)
                                                                               AACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTAT                                                                       >  NB501016:282:H2V7GAFX2:1:11105:14221:11836/1‑151 (MQ=21)
                                                                                ACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC                                                                                                                     >  NB501016:282:H2V7GAFX2:4:21406:2372:10773/1‑104 (MQ=3)
                                                                                 CCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGC                                                                     >  NB501016:282:H2V7GAFX2:1:11103:2464:15058/1‑151 (MQ=22)
                                                                                   TGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCT                                                                   >  NB501016:282:H2V7GAFX2:3:21607:16815:9249/1‑151 (MQ=22)
                                                                                       CGGCCCTTGGTGGCGCGGTTAACCTCCACTTACTTCGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCG                                                               <  NB501016:282:H2V7GAFX2:4:21404:23889:8670/151‑1 (MQ=20)
                                                                                       CGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCG                                                               >  NB501016:282:H2V7GAFX2:3:11512:15252:15913/1‑151 (MQ=21)
                                                                                       CGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCG                                                               <  NB501016:282:H2V7GAFX2:4:21609:13560:12794/151‑1 (MQ=22)
                                                                                         GCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGG                                                              >  NB501016:282:H2V7GAFX2:1:21201:17023:13047/1‑150 (MQ=22)
                                                                                          CCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGAT                                                            >  NB501016:282:H2V7GAFX2:2:21207:19233:11783/1‑151 (MQ=22)
                                                                                           CCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATC                                                           <  NB501016:282:H2V7GAFX2:2:21308:13424:16982/151‑1 (MQ=21)
                                                                                           CCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATC                                                           <  NB501016:282:H2V7GAFX2:1:21207:12133:14231/151‑1 (MQ=22)
                                                                                              TGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTA                                                         <  NB501016:282:H2V7GAFX2:4:21412:4070:10153/150‑1 (MQ=22)
                                                                                               GGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACG                                                       <  NB501016:282:H2V7GAFX2:2:11201:2339:11546/151‑1 (MQ=21)
                                                                                                 TGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTC                                                     >  NB501016:282:H2V7GAFX2:2:21201:18529:11348/1‑151 (MQ=21)
                                                                                                    CGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTG                                                                            <  NB501016:282:H2V7GAFX2:1:21108:16685:15136/125‑1 (MQ=23)
                                                                                                     GCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAG                                                 <  NB501016:282:H2V7GAFX2:2:21206:10760:8203/151‑1 (MQ=21)
                                                                                                        GTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTA                                                         <  NB501016:282:H2V7GAFX2:4:11410:25277:8657/140‑1 (MQ=22)
                                                                                                           AACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCC                                           <  NB501016:282:H2V7GAFX2:1:11310:17957:6913/151‑1 (MQ=22)
                                                                                                            ACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCG                                          <  NB501016:282:H2V7GAFX2:4:11502:24259:13132/151‑1 (MQ=21)
                                                                                                              CGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTG                                        <  NB501016:282:H2V7GAFX2:2:11210:16159:13101/151‑1 (MQ=21)
                                                                                                              CGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTG                                        >  NB501016:282:H2V7GAFX2:4:11402:2127:8133/1‑151 (MQ=22)
                                                                                                                CCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCA                                      <  NB501016:282:H2V7GAFX2:4:21510:8847:11202/151‑1 (MQ=22)
                                                                                                                  ACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAG                                    <  NB501016:282:H2V7GAFX2:2:21303:12388:7933/151‑1 (MQ=21)
                                                                                                                  ACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAG                                    <  NB501016:282:H2V7GAFX2:4:21402:15109:2436/151‑1 (MQ=22)
                                                                                                                     TACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTAT                                 >  NB501016:282:H2V7GAFX2:1:11301:3865:14794/1‑151 (MQ=22)
                                                                                                                     TACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGT                                   <  NB501016:282:H2V7GAFX2:1:21311:22610:14529/149‑1 (MQ=22)
                                                                                                                         ACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGG                             >  NB501016:282:H2V7GAFX2:2:11110:1646:4042/1‑151 (MQ=21)
                                                                                                                         ACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGG                              <  NB501016:282:H2V7GAFX2:4:21607:7145:11033/150‑1 (MQ=22)
                                                                                                                         ACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTG                               <  NB501016:282:H2V7GAFX2:3:11407:16279:11021/149‑1 (MQ=22)
                                                                                                                           GGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCT                           >  NB501016:282:H2V7GAFX2:2:21101:18866:5572/1‑151 (MQ=21)
                                                                                                                                CGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGG                       <  NB501016:282:H2V7GAFX2:2:21305:19969:18517/150‑1 (MQ=21)
                                                                                                                                CGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGG                       <  NB501016:282:H2V7GAFX2:3:11401:14708:7382/150‑1 (MQ=22)
                                                                                                                                 GGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCG                     >  NB501016:282:H2V7GAFX2:4:11412:5051:9776/1‑151 (MQ=21)
                                                                                                                                 GGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCG                     >  NB501016:282:H2V7GAFX2:1:21207:24601:1239/1‑151 (MQ=21)
                                                                                                                                      ACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTG                <  NB501016:282:H2V7GAFX2:1:11302:20281:20018/151‑1 (MQ=22)
                                                                                                                                      ACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTG                <  NB501016:282:H2V7GAFX2:1:11308:17893:2540/151‑1 (MQ=22)
                                                                                                                                         TCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTG              <  NB501016:282:H2V7GAFX2:1:21104:20242:7493/150‑1 (MQ=21)
                                                                                                                                            GCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCA           <  NB501016:282:H2V7GAFX2:4:21403:23025:10203/150‑1 (MQ=22)
                                                                                                                                            GCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCC            <  NB501016:282:H2V7GAFX2:2:21208:15850:2541/149‑1 (MQ=22)
                                                                                                                                            GCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCC            <  NB501016:282:H2V7GAFX2:1:11309:25026:17719/149‑1 (MQ=22)
                                                                                                                                             CACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACC         <  NB501016:282:H2V7GAFX2:2:11112:10731:12296/151‑1 (MQ=21)
                                                                                                                                             CACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACC         <  NB501016:282:H2V7GAFX2:2:21112:8924:5713/151‑1 (MQ=22)
                                                                                                                                               CGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTC       >  NB501016:282:H2V7GAFX2:4:21611:5501:17875/1‑151 (MQ=21)
                                                                                                                                                 CTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAC     >  NB501016:282:H2V7GAFX2:3:21606:15085:13011/1‑151 (MQ=21)
                                                                                                                                                 CTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCAC     >  NB501016:282:H2V7GAFX2:3:11505:3447:7701/1‑151 (MQ=21)
                                                                                                                                                    GTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCCC  >  NB501016:282:H2V7GAFX2:2:21109:7218:8141/1‑151 (MQ=21)
                                                                                                                                                    |                                                                                                                                                      
GATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCCC  >  NC_002947_CJ‑RC/4348664‑4348962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑