Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F13 I1 R1 58 75.1 3211970 98.6% 3167002 146.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947_CJ‑RC 2,958,523 C→T A428V (GCA→GTA)  PP_2589 → aldehyde dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC2,958,5230CT100.0% 184.0 / NA 54A428V (GCA→GTA) PP_2589aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/28);  total (26/28)

CTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTT  >  NC_002947_CJ‑RC/2958377‑2958659
                                                                                                                                                  |                                                                                                                                        
cTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAg                                                                                                                                        >  7:282397/1‑149 (MQ=255)
cTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAg                                                                                                                                        >  5:309604/1‑149 (MQ=255)
 tACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGc                                                                                                                                       >  4:12896/1‑149 (MQ=255)
     tCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCg                                                                                                                                   <  2:254063/149‑1 (MQ=255)
       gAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGgt                                                                                                                                 <  8:282397/149‑1 (MQ=255)
       gAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGgt                                                                                                                                 >  1:130275/1‑149 (MQ=255)
         gCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGgtgt                                                                                                                               <  4:281499/149‑1 (MQ=255)
            gACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGtgtg                                                                                                                              >  5:278977/1‑147 (MQ=255)
            gACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGa                                                                                                                            <  7:123997/149‑1 (MQ=255)
               cATTTTTGCCGGTGTCGATGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGcg                                                                                                                         <  8:98632/149‑1 (MQ=255)
                   tttGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGcgacg                                                                                                                      <  5:387228/148‑1 (MQ=255)
                       ccGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACtt                                                                                                                  >  4:120247/1‑148 (MQ=255)
                          gtgtCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAAc                                                                                                              <  7:5744/149‑1 (MQ=255)
                           tgtCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAAc                                                                                                              >  6:94796/1‑148 (MQ=255)
                           tgtCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAAc                                                                                                              >  4:51505/1‑148 (MQ=255)
                             tCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAg                                                                                                           >  4:196654/1‑149 (MQ=255)
                               gAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGt                                                                                                         >  3:199324/1‑149 (MQ=255)
                               gAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGt                                                                                                         >  4:19044/1‑149 (MQ=255)
                                aGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTg                                                                                                        <  2:130275/149‑1 (MQ=255)
                                 gggCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTg                                                                                                        >  7:26935/1‑148 (MQ=255)
                                     cGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCg                                                                                                   <  6:320880/149‑1 (MQ=255)
                                     cGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCg                                                                                                   <  1:396986/149‑1 (MQ=255)
                                        aTGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCggg                                                                                                 >  1:245667/1‑148 (MQ=255)
                                              cTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGcgcg                                                                                          <  7:140256/149‑1 (MQ=255)
                                                    cgcgAGGATGTGTTCGGCCCGGTACTGGCGGTCAGCGACTTCGACACGGAAGAAGAAGCCGTGCGCCTGTCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGtt                                                                                    <  8:398246/149‑1 (MQ=255)
                                                         ggaggTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAg                                                                               >  2:119539/1‑149 (MQ=255)
                                                           aggTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGcc                                                                             <  6:309604/149‑1 (MQ=255)
                                                               gtTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTa                                                                         >  1:29064/1‑149 (MQ=255)
                                                                   ggCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCg                                                                      >  8:5904/1‑148 (MQ=255)
                                                                                  gTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGt                                                      <  7:97189/149‑1 (MQ=255)
                                                                                   tCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTc                                                     <  3:196654/149‑1 (MQ=255)
                                                                                        gcCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCggg                                                                                                 >  5:82632/1‑100 (MQ=255)
                                                                                        gcCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCggg                                                                                                 <  6:82632/100‑1 (MQ=255)
                                                                                          cTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGaaa                                                                                <  6:200663/115‑1 (MQ=255)
                                                                                          cTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGaaa                                                                                >  5:200663/1‑115 (MQ=255)
                                                                                          cTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATgcgc                                              <  1:210596/149‑1 (MQ=255)
                                                                                              gACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTgg                                           <  7:185861/148‑1 (MQ=255)
                                                                                              gACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGa                                          >  4:170408/1‑149 (MQ=255)
                                                                                               acacGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGAt                                         <  3:120247/149‑1 (MQ=255)
                                                                                                 acGGAAGAAGAAGCCGTGCGCCTGGCAAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGAtgt                                       >  2:380293/1‑149 (MQ=255)
                                                                                                  cGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGACTGGTAGCCTCGGTGTGGAGCGAAGACTTCAACCAGGTGCTCCGGGTAGCGCTGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGAtgtg                                      <  8:81130/149‑1 (MQ=255)
                                                                                                   ggAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGa                                     >  5:161287/1‑149 (MQ=255)
                                                                                                     aagaagaagCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGTTGTGACg                                   >  7:285037/1‑149 (MQ=255)
                                                                                                             gCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGtt                           <  1:128119/149‑1 (MQ=255)
                                                                                                                gTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCgg                        >  1:34098/1‑149 (MQ=255)
                                                                                                                gTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCgg                        >  7:292192/1‑149 (MQ=255)
                                                                                                                    gcCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTggg                    >  8:371247/1‑149 (MQ=255)
                                                                                                                      cTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTggggg                  <  6:278977/149‑1 (MQ=255)
                                                                                                                         gcaaaCGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCaa               <  1:380293/146‑1 (MQ=255)
                                                                                                                           cAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTggg                    >  6:262023/1‑142 (MQ=255)
                                                                                                                           cAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTggg                    <  5:262023/142‑1 (MQ=255)
                                                                                                                            aaCGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCa            <  6:47800/149‑1 (MQ=255)
                                                                                                                                          tGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCTGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTTAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGttt  <  4:198756/145‑1 (MQ=255)
                                                                                                                                          tGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGttt  <  1:119539/145‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                        
CTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTT  >  NC_002947_CJ‑RC/2958377‑2958659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTG  >  NC_002947_CJ‑RC/2958373‑2958660
                                                                                                                                                      |                                                                                                                                         
ACAACTTCATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTGCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGT                                                                                                                                           <  NB501016:282:H2V7GAFX2:1:11301:2861:16858/151‑1 (MQ=60)
   ACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGC                                                                                                                                        >  NB501016:282:H2V7GAFX2:4:21502:12404:3370/1‑151 (MQ=60)
   ACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGC                                                                                                                                        >  NB501016:282:H2V7GAFX2:3:21508:8287:15819/1‑151 (MQ=60)
          CGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTG                                                                                                                                 >  NB501016:282:H2V7GAFX2:1:11210:4651:14409/1‑151 (MQ=60)
               CGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAG                                                                                                                            <  NB501016:282:H2V7GAFX2:4:11510:19078:4870/151‑1 (MQ=60)
               CGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGG                                                                                                                              >  NB501016:282:H2V7GAFX2:3:21503:20187:6019/1‑149 (MQ=60)
                      TTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGA                                                                                                                      <  NB501016:282:H2V7GAFX2:3:21610:14765:2537/150‑1 (MQ=60)
                             GGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACC                                                                                                              <  NB501016:282:H2V7GAFX2:4:11402:16120:1307/151‑1 (MQ=60)
                                  CGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTG                                                                                                         >  NB501016:282:H2V7GAFX2:2:11311:21725:18251/1‑151 (MQ=60)
                                    AGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGC                                                                                                        >  NB501016:282:H2V7GAFX2:4:11405:9544:14151/1‑150 (MQ=60)
                                        CCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGG                                                                                                   <  NB501016:282:H2V7GAFX2:1:21311:1565:17514/151‑1 (MQ=60)
                                           CATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGT                                                                                                 >  NB501016:282:H2V7GAFX2:1:21107:26076:7425/1‑150 (MQ=60)
                                                 CCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGG                                                                                          <  NB501016:282:H2V7GAFX2:4:11512:1600:18976/151‑1 (MQ=60)
                                                                  TGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTAC                                                                         >  NB501016:282:H2V7GAFX2:1:11105:6660:14221/1‑151 (MQ=60)
                                                                                     GGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTC                                                      <  NB501016:282:H2V7GAFX2:4:11505:20039:13314/151‑1 (MQ=60)
                                                                                      GTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCG                                                     <  NB501016:282:H2V7GAFX2:2:11311:4758:9706/151‑1 (MQ=60)
                                                                                           CGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGT                                                                                                 >  NB501016:282:H2V7GAFX2:3:11504:24722:5149/1‑102 (MQ=60)
                                                                                             CCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCT                                              <  NB501016:282:H2V7GAFX2:1:21101:21418:6041/151‑1 (MQ=60)
                                                                                             CCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAG                                                                                >  NB501016:282:H2V7GAFX2:3:11611:24793:20193/1‑117 (MQ=60)
                                                                                                 CGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGA                                           <  NB501016:282:H2V7GAFX2:4:11609:8763:4664/150‑1 (MQ=60)
                                                                                                  GACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATG                                         <  NB501016:282:H2V7GAFX2:2:11210:11600:9329/151‑1 (MQ=60)
                                                                                                      CGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGAC                                     >  NB501016:282:H2V7GAFX2:3:11605:2606:9615/1‑151 (MQ=60)
                                                                                                        GAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGTTGTGACGG                                   >  NB501016:282:H2V7GAFX2:4:21502:26293:12201/1‑151 (MQ=60)
                                                                                                                AGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTC                           <  NB501016:282:H2V7GAFX2:1:11210:11256:7285/151‑1 (MQ=60)
                                                                                                                   CGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGT                        >  NB501016:282:H2V7GAFX2:4:21503:24393:17309/1‑151 (MQ=60)
                                                                                                                   CGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGT                        >  NB501016:282:H2V7GAFX2:1:11106:19332:10838/1‑151 (MQ=60)
                                                                                                                            GGCAAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAG               <  NB501016:282:H2V7GAFX2:1:21308:15240:13402/151‑1 (MQ=60)
                                                                                                                              CCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGG                    <  NB501016:282:H2V7GAFX2:3:21412:19813:4157/144‑1 (MQ=60)
                                                                                                                                             ATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTG  <  NB501016:282:H2V7GAFX2:1:11208:2081:16900/147‑1 (MQ=60)
                                                                                                                                                      |                                                                                                                                         
ACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTG  >  NC_002947_CJ‑RC/2958373‑2958660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑