Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F61 I1 R1 63 66.6 2847048 98.6% 2807189 146.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947_CJ‑RC 2,958,523 C→T A428V (GCA→GTA)  PP_2589 → aldehyde dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC2,958,5230CT100.0% 230.0 / NA 67A428V (GCA→GTA) PP_2589aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/33);  total (34/33)

TACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGC  >  NC_002947_CJ‑RC/2958378‑2958665
                                                                                                                                                 |                                                                                                                                              
tACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGc                                                                                                                                             >  2:131845/1‑149 (MQ=255)
 aCATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGcc                                                                                                                                            >  6:149723/1‑149 (MQ=255)
      gAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGgt                                                                                                                                       <  4:158358/149‑1 (MQ=255)
      gAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGgt                                                                                                                                       <  3:54366/149‑1 (MQ=255)
         ccGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGtgtg                                                                                                                                    >  4:163847/1‑149 (MQ=255)
            aacATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAg                                                                                                                                 >  5:181733/3‑149 (MQ=255)
            aCCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGa                                                                                                                                  >  7:335145/1‑148 (MQ=255)
            aCCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAg                                                                                                                                 >  5:335078/1‑149 (MQ=255)
             ccATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGc                                                                                                                                <  1:277130/149‑1 (MQ=255)
              cATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGc                                                                                                                                >  3:310716/1‑148 (MQ=255)
               aTTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGcga                                                                                                                              >  5:287861/1‑149 (MQ=255)
                 ttttGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGcgac                                                                                                                             <  7:362783/148‑1 (MQ=255)
                  tttGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGcgac                                                                                                                             <  7:323158/147‑1 (MQ=255)
                   ttGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGcgacga                                                                                                                           <  3:219804/148‑1 (MQ=255)
                     gCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCgacgac                                                                                                                          <  7:165890/147‑1 (MQ=255)
                     gCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACt                                                                                                                         <  4:256528/148‑1 (MQ=255)
                      ccGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTc                                                                                                                       <  3:133533/149‑1 (MQ=255)
                       cGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGCGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACtt                                                                                                                        >  7:317926/1‑147 (MQ=255)
                            tCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAg                                                                                                                 <  6:287861/149‑1 (MQ=255)
                             cGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAg                                                                                                                 <  8:99277/148‑1 (MQ=255)
                                       aTGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCggg                                                                                                       <  8:328496/148‑1 (MQ=255)
                                           gCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAgcg                                                                                                  >  1:51240/1‑149 (MQ=255)
                                                  gcgcgAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGt                                                                                           <  5:10934/149‑1 (MQ=255)
                                                   cgcgAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCATCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGt                                                                                           >  7:188161/1‑148 (MQ=255)
                                                        ggaggTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAg                                                                                     <  1:297747/149‑1 (MQ=255)
                                                          aggTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGcc                                                                                   <  4:310716/149‑1 (MQ=255)
                                                           ggTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCg                                                                                  <  6:11750/149‑1 (MQ=255)
                                                             tgtTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGAAGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCgg                                                                                 >  4:2433/1‑148 (MQ=255)
                                                                 cGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCg                                                                            >  2:326266/1‑149 (MQ=255)
                                                                    cccGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTAt                                                                         >  3:260550/1‑149 (MQ=255)
                                                                        gTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGt                                                                     >  4:24295/1‑149 (MQ=255)
                                                                         tACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTg                                                                    <  6:219723/149‑1 (MQ=255)
                                                                              gCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAaca                                                                >  2:331820/1‑148 (MQ=255)
                                                                                     gcgcCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGa                                                         >  8:312765/1‑148 (MQ=255)
                                                                                     gcgcCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGAt                                                        <  3:265777/149‑1 (MQ=255)
                                                                                       gcCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGgtgt                                                                                                                                     >  6:310829/1‑70 (MQ=255)
                                                                                       gcCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGgtgt                                                                                                                                     <  5:310829/70‑1 (MQ=255)
                                                                                        ccTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATgcg                                                     >  5:126562/1‑149 (MQ=255)
                                                                                        ccTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATgcg                                                     >  5:131855/1‑149 (MQ=255)
                                                                                        ccTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATgcg                                                     >  6:10916/1‑149 (MQ=255)
                                                                                         cTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATgcgc                                                    >  6:251050/1‑149 (MQ=255)
                                                                                            cGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTg                                                  >  2:83966/1‑148 (MQ=255)
                                                                                             gACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTgg                                                 <  5:302028/148‑1 (MQ=255)
                                                                                              acacGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGa                                                >  7:288472/1‑148 (MQ=255)
                                                                                               cacGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGAtg                                              <  2:18343/149‑1 (MQ=255)
                                                                                                             ccGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTc                                >  6:15147/1‑149 (MQ=255)
                                                                                                              cGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCg                               <  6:181733/149‑1 (MQ=255)
                                                                                                                  cgcCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTgg                           <  5:149723/149‑1 (MQ=255)
                                                                                                                  cgcCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTg                            <  5:10916/148‑1 (MQ=255)
                                                                                                                   gcCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTggg                          <  3:147670/149‑1 (MQ=255)
                                                                                                                    ccTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTgggg                         <  6:335078/149‑1 (MQ=255)
                                                                                                                        gCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTggggg                        >  7:98540/1‑146 (MQ=255)
                                                                                                                               aCAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTc               <  7:353625/148‑1 (MQ=255)
                                                                                                                                cAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCgg             <  3:24295/149‑1 (MQ=255)
                                                                                                                                 aGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCggg            >  1:15558/1‑149 (MQ=255)
                                                                                                                                  gCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCgggg           <  2:51240/149‑1 (MQ=255)
                                                                                                                                   cATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTc               >  8:253867/1‑144 (MQ=255)
                                                                                                                                    aTCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCg              <  7:253867/144‑1 (MQ=255)
                                                                                                                                    aTCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGtt         >  5:64949/1‑149 (MQ=255)
                                                                                                                                     tCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTc               >  8:266793/1‑142 (MQ=255)
                                                                                                                                      cTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCg              <  7:266793/142‑1 (MQ=255)
                                                                                                                                        aTGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTgg      >  8:355810/1‑148 (MQ=255)
                                                                                                                                          ggCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGcc    >  8:133059/1‑148 (MQ=255)
                                                                                                                                          ggCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCcg   >  4:270686/1‑149 (MQ=255)
                                                                                                                                           gCCTGGTTGCGTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGATAGCCGGTACCGTATCGGTTAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCTGTCGGGGTTTGGCcgc  <  4:31345/149‑1 (MQ=255)
                                                                                                                                           gCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGcc                                                                                   >  3:140637/1‑68 (MQ=255)
                                                                                                                                           gCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGcc                                                                                   <  4:140637/68‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGC  >  NC_002947_CJ‑RC/2958378‑2958665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTG  >  NC_002947_CJ‑RC/2958375‑2958671
                                                                                                                                                    |                                                                                                                                                    
AACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAG                                                                                                                                                    <  NB501016:282:H2V7GAFX2:4:21511:7187:5033/151‑1 (MQ=60)
        CGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTG                                                                                                                                            <  NB501016:282:H2V7GAFX2:2:11112:11728:4836/151‑1 (MQ=60)
              GACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGC                                                                                                                                      >  NB501016:282:H2V7GAFX2:4:21607:18882:2510/1‑151 (MQ=60)
              GACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGC                                                                                                                                      >  NB501016:282:H2V7GAFX2:3:21608:14868:2088/1‑151 (MQ=60)
              GAACATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGC                                                                                                                                      >  NB501016:282:H2V7GAFX2:3:11612:25065:11492/1‑151 (MQ=60)
               ACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCG                                                                                                                                     <  NB501016:282:H2V7GAFX2:1:21207:18932:15436/151‑1 (MQ=60)
                CCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCG                                                                                                                                     >  NB501016:282:H2V7GAFX2:2:21306:5814:11717/1‑150 (MQ=60)
                 CATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGAC                                                                                                                                   >  NB501016:282:H2V7GAFX2:3:21510:17042:18598/1‑151 (MQ=60)
                  ATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACG                                                                                                                                  <  NB501016:282:H2V7GAFX2:4:21612:11381:14996/151‑1 (MQ=60)
                   TTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACG                                                                                                                                  <  NB501016:282:H2V7GAFX2:4:21604:18343:12423/150‑1 (MQ=60)
                     TTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGAC                                                                                                                                <  NB501016:282:H2V7GAFX2:2:21109:6572:9193/150‑1 (MQ=60)
                      TTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACT                                                                                                                               <  NB501016:282:H2V7GAFX2:4:11609:10584:11339/150‑1 (MQ=60)
                        GCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCA                                                                                                                            <  NB501016:282:H2V7GAFX2:2:11304:9421:2030/151‑1 (MQ=60)
                         CCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGCGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCA                                                                                                                            >  NB501016:282:H2V7GAFX2:4:21603:19302:11303/1‑150 (MQ=60)
                                             AGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGC                                                                                                       >  NB501016:282:H2V7GAFX2:1:11110:11843:12175/1‑151 (MQ=60)
                                                    CGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTT                                                                                                <  NB501016:282:H2V7GAFX2:3:11403:14520:4655/151‑1 (MQ=60)
                                                     GCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCATCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTG                                                                                               >  NB501016:282:H2V7GAFX2:4:21402:11541:1666/1‑151 (MQ=60)
                                                          AGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGC                                                                                          <  NB501016:282:H2V7GAFX2:1:21211:20831:18491/151‑1 (MQ=60)
                                                                      GCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATC                                                                              >  NB501016:282:H2V7GAFX2:2:21207:22177:16426/1‑151 (MQ=60)
                                                                                       AGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATG                                                             <  NB501016:282:H2V7GAFX2:2:21208:14228:17674/151‑1 (MQ=60)
                                                                                         CGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTG                                                                                                                                          <  NB501016:282:H2V7GAFX2:3:21603:16659:8883/72‑1 (MQ=60)
                                                                                          GCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGC                                                          >  NB501016:282:H2V7GAFX2:3:11602:26839:5069/1‑151 (MQ=60)
                                                                                          GCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGC                                                          >  NB501016:282:H2V7GAFX2:3:11603:1160:5078/1‑151 (MQ=60)
                                                                                               CGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGA                                                      <  NB501016:282:H2V7GAFX2:3:21601:8502:15418/150‑1 (MQ=60)
                                                                                                GACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATG                                                    >  NB501016:282:H2V7GAFX2:4:21509:5437:13554/1‑151 (MQ=60)
                                                                                                                    GCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGG                                <  NB501016:282:H2V7GAFX2:3:11606:5528:10821/151‑1 (MQ=60)
                                                                                                                    GCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGG                                 <  NB501016:282:H2V7GAFX2:3:11403:12364:4565/150‑1 (MQ=60)
                                                                                                                     CGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGG                               <  NB501016:282:H2V7GAFX2:2:11306:25153:15235/151‑1 (MQ=60)
                                                                                                                          GGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCA                            >  NB501016:282:H2V7GAFX2:4:11507:11146:20333/1‑149 (MQ=60)
                                                                                                                                CGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCG                    <  NB501016:282:H2V7GAFX2:4:21610:22157:17298/151‑1 (MQ=60)
                                                                                                                                  ACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGG                  <  NB501016:282:H2V7GAFX2:2:11106:17536:1833/151‑1 (MQ=60)
                                                                                                                                   CAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGG                 >  NB501016:282:H2V7GAFX2:1:11103:7176:18174/1‑151 (MQ=60)
                                                                                                                                     GCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGG                   <  NB501016:282:H2V7GAFX2:4:21502:16065:14393/147‑1 (MQ=60)
                                                                                                                                      CATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTT              >  NB501016:282:H2V7GAFX2:3:11502:19220:5185/1‑151 (MQ=60)
                                                                                                                                       ATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGG                   <  NB501016:282:H2V7GAFX2:4:21505:21893:6991/145‑1 (MQ=60)
                                                                                                                                             GGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCG                                                                                        >  NB501016:282:H2V7GAFX2:2:11305:6884:9077/1‑70 (MQ=60)
                                                                                                                                                  GGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTG  <  NB501016:282:H2V7GAFX2:4:21602:12022:10591/151‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
AACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTG  >  NC_002947_CJ‑RC/2958375‑2958671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑