Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F25 I1 R1 61 74.4 3161000 98.6% 3116746 146.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947_CJ‑RC 2,958,523 C→T A428V (GCA→GTA)  PP_2589 → aldehyde dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC2,958,5230CT100.0% 280.2 / NA 80A428V (GCA→GTA) PP_2589aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/43);  total (37/43)

TCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGC  >  NC_002947_CJ‑RC/2958382‑2958665
                                                                                                                                             |                                                                                                                                              
tCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCg                                                                                                                                         <  7:143858/149‑1 (MQ=255)
  gAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGgt                                                                                                                                       <  8:393364/149‑1 (MQ=255)
  gAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGgt                                                                                                                                       <  4:129978/149‑1 (MQ=255)
     ccGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGtgtg                                                                                                                                    >  3:341623/1‑149 (MQ=255)
       gACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGtgtg                                                                                                                                    >  4:513/1‑147 (MQ=255)
        aCCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAg                                                                                                                                 >  6:15036/1‑149 (MQ=255)
        aCCATTTTTGCCGGTGTCGAGGGCCGCAGGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAg                                                                                                                                 >  8:96769/1‑149 (MQ=255)
         ccATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGc                                                                                                                                >  5:192537/1‑149 (MQ=255)
         ccATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGc                                                                                                                                <  5:384130/149‑1 (MQ=255)
            tttttGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGcgac                                                                                                                             <  8:378085/149‑1 (MQ=255)
                tGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCgacgac                                                                                                                          <  5:44125/148‑1 (MQ=255)
                 gCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACt                                                                                                                         <  2:247078/148‑1 (MQ=255)
                  ccGGTGTCGAGGGCCTCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTc                                                                                                                       <  5:231019/149‑1 (MQ=255)
                  ccGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTc                                                                                                                       <  2:178023/149‑1 (MQ=255)
                    ggTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCaa                                                                                                                     <  6:38673/149‑1 (MQ=255)
                    ggTGTCGAGGGCCGCAGGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCaa                                                                                                                     <  7:96769/149‑1 (MQ=255)
                        tCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAg                                                                                                                 <  6:148653/149‑1 (MQ=255)
                        tCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAg                                                                                                                 <  7:116178/149‑1 (MQ=255)
                          gAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAgg                                                                                                                <  7:1576/148‑1 (MQ=255)
                          gAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGt                                                                                                               >  7:221847/1‑149 (MQ=255)
                            gggCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTg                                                                                                              >  6:111486/1‑148 (MQ=255)
                               ccGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCAcc                                                                                                          >  3:91247/1‑149 (MQ=255)
                                cGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCg                                                                                                         >  7:360529/1‑149 (MQ=255)
                                cGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCg                                                                                                         <  4:281993/149‑1 (MQ=255)
                                 gCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCgg                                                                                                        >  2:34881/1‑149 (MQ=255)
                                  cATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCggg                                                                                                       <  1:290905/149‑1 (MQ=255)
                                    tGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTa                                                                                                     <  7:286604/149‑1 (MQ=255)
                                           ggCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCTCCGGGTAGCGCggg                                                                                              >  3:130463/1‑149 (MQ=255)
                                            gcgcgcGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCgg                                                                                               <  4:91247/147‑1 (MQ=255)
                                            gcgcgcGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGc                                                                                             <  3:238466/149‑1 (MQ=255)
                                              gcgcgAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGt                                                                                           >  3:191205/1‑149 (MQ=255)
                                                 cgAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTg                                                                                         >  1:301592/1‑148 (MQ=255)
                                                   aggaggTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGaaa                                                                                      <  1:70730/149‑1 (MQ=255)
                                                       ggTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCg                                                                                  <  4:374571/149‑1 (MQ=255)
                                                       ggTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCg                                                                                  <  8:94962/149‑1 (MQ=255)
                                                        gtgtTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGTCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCg                                                                                  <  8:160306/148‑1 (MQ=255)
                                                         tgtTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCgg                                                                                 >  6:73325/1‑148 (MQ=255)
                                                           ttCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTAc                                                                              <  5:238499/149‑1 (MQ=255)
                                                                 ccGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCAGGCGTTGAAAGCCGGTACCGTATc                                                                        >  2:151367/1‑149 (MQ=255)
                                                                  cGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCg                                                                       <  2:185598/149‑1 (MQ=255)
                                                                   ggTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCgg                                                                      <  8:360529/149‑1 (MQ=255)
                                                                     tACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATGTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTg                                                                    <  5:309106/149‑1 (MQ=255)
                                                                     tACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGt                                                                     >  5:206775/1‑148 (MQ=255)
                                                                          gCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACATCATCTCTGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAac                                                                 <  3:119390/147‑1 (MQ=255)
                                                                          gCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAaca                                                                <  1:34881/148‑1 (MQ=255)
                                                                          gCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAaca                                                                >  8:337192/1‑148 (MQ=255)
                                                                          gCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAaca                                                                >  7:347836/1‑148 (MQ=255)
                                                                                      ttCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCt                                                   >  5:393030/1‑149 (MQ=255)
                                                                                       tCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTg                                                  >  8:271921/1‑149 (MQ=255)
                                                                                        cGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCt                                                   <  5:15036/147‑1 (MQ=255)
                                                                                          acacGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGAt                                               <  4:341623/149‑1 (MQ=255)
                                                                                          acacGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGAt                                               >  4:226666/1‑149 (MQ=255)
                                                                                           cacGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTa                                                                                                     >  3:246187/1‑94 (MQ=255)
                                                                                           cacGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTa                                                                                                     <  4:246187/94‑1 (MQ=255)
                                                                                            acGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGa                                                >  8:221096/1‑146 (MQ=255)
                                                                                            acGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGa                                                <  7:221096/146‑1 (MQ=255)
                                                                                               gaagaagaaGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGAc                                          >  1:50510/1‑149 (MQ=255)
                                                                                                    agaagCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGc                                     >  5:72803/1‑149 (MQ=255)
                                                                                                     gaagCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGcc                                    >  6:368740/1‑149 (MQ=255)
                                                                                                          cGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCg                               >  1:137255/1‑149 (MQ=255)
                                                                                                          cGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACAGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCg                               >  4:57993/1‑149 (MQ=255)
                                                                                                            tGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGt                             <  6:136815/149‑1 (MQ=255)
                                                                                                               gcCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTggg                          <  6:72803/149‑1 (MQ=255)
                                                                                                                ccTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTTCCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTgggg                         <  7:84263/149‑1 (MQ=255)
                                                                                                                 cTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACGGTATCGGTGAGCACAGTCGATGCGCTGGATGTGACGGTGCCGGTCgggggggg                        >  4:258682/1‑149 (MQ=255)
                                                                                                                 cTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTggggg                        >  1:321622/1‑149 (MQ=255)
                                                                                                                 cTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCAGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTggggg                        <  1:151367/149‑1 (MQ=255)
                                                                                                                  tGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTggggg                        >  5:302427/1‑148 (MQ=255)
                                                                                                                   ggCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGc                       >  3:17237/1‑148 (MQ=255)
                                                                                                                    gCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGc                       >  3:286043/1‑147 (MQ=255)
                                                                                                                    gCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGc                       >  1:372390/1‑147 (MQ=255)
                                                                                                                          gACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTc               >  1:104085/1‑149 (MQ=255)
                                                                                                                           aCAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCg              <  3:513/149‑1 (MQ=255)
                                                                                                                            cAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCTCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCgg             <  5:111486/149‑1 (MQ=255)
                                                                                                                            cAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCgg             <  7:178362/149‑1 (MQ=255)
                                                                                                                             aGCATCTATGGCCTTGTAGCCCAGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGTCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTAGATGCGCTGGATGTGACGGTGCCGTTCGTTGGGGGCAAGCAGTCggg            <  8:92094/149‑1 (MQ=255)
                                                                                                                             aGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCggg            <  7:45481/149‑1 (MQ=255)
                                                                                                                                   tATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTgg      >  7:119893/1‑149 (MQ=255)
                                                                                                                                     tGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTgg      <  6:192537/147‑1 (MQ=255)
                                                                                                                                       gCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCcgc  >  6:201813/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                              
TCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGC  >  NC_002947_CJ‑RC/2958382‑2958665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGC  >  NC_002947_CJ‑RC/2958381‑2958662
                                                                                                                                              |                                                                                                                                           
ATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGG                                                                                                                                     <  NB501016:282:H2V7GAFX2:4:11602:6265:2412/151‑1 (MQ=60)
     GCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGG                                                                                                                                >  NB501016:282:H2V7GAFX2:2:21306:13555:7185/1‑151 (MQ=60)
         ACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCG                                                                                                                            <  NB501016:282:H2V7GAFX2:3:21610:7508:4001/151‑1 (MQ=60)
         ACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCG                                                                                                                            >  NB501016:282:H2V7GAFX2:3:11611:23290:3903/1‑151 (MQ=60)
                TTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACT                                                                                                                      <  NB501016:282:H2V7GAFX2:3:11409:1476:2131/150‑1 (MQ=60)
                  GCCGGTGTCGAGGGCCTCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCA                                                                                                                   <  NB501016:282:H2V7GAFX2:3:21406:21230:8717/151‑1 (MQ=60)
                    CGGTGTCGAGGGCCGCAGGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAAC                                                                                                                 <  NB501016:282:H2V7GAFX2:4:11505:21172:12874/151‑1 (MQ=60)
                        GTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGG                                                                                                             <  NB501016:282:H2V7GAFX2:4:11509:21813:3353/151‑1 (MQ=60)
                          CGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTG                                                                                                           >  NB501016:282:H2V7GAFX2:4:21404:2968:5170/1‑151 (MQ=60)
                          CGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGT                                                                                                            <  NB501016:282:H2V7GAFX2:4:11401:25002:6271/150‑1 (MQ=60)
                               GCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCG                                                                                                      >  NB501016:282:H2V7GAFX2:2:11206:15116:1634/1‑151 (MQ=60)
                                CCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGG                                                                                                     >  NB501016:282:H2V7GAFX2:4:21604:13438:3834/1‑151 (MQ=60)
                                  GCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGT                                                                                                   <  NB501016:282:H2V7GAFX2:1:21204:3570:19627/151‑1 (MQ=60)
                                    ATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAG                                                                                                 <  NB501016:282:H2V7GAFX2:4:21503:17450:1682/151‑1 (MQ=60)
                                           TGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCTCCGGGTAGCGCGGGC                                                                                          >  NB501016:282:H2V7GAFX2:2:11301:7573:3303/1‑151 (MQ=60)
                                            GGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCG                                                                                         <  NB501016:282:H2V7GAFX2:2:21108:8072:14203/151‑1 (MQ=60)
                                              CGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTT                                                                                       >  NB501016:282:H2V7GAFX2:2:11311:13333:13699/1‑151 (MQ=60)
                                                 GCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGA                                                                                     >  NB501016:282:H2V7GAFX2:1:21206:19873:19699/1‑150 (MQ=60)
                                                   GAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAG                                                                                  <  NB501016:282:H2V7GAFX2:1:11112:8607:20168/151‑1 (MQ=60)
                                                           GTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACC                                                                          <  NB501016:282:H2V7GAFX2:3:21407:24173:16536/151‑1 (MQ=60)
                                                                     GTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATGTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGA                                                                <  NB501016:282:H2V7GAFX2:3:21508:19471:19453/151‑1 (MQ=60)
                                                                     GTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTG                                                                 >  NB501016:282:H2V7GAFX2:3:21401:13583:15028/1‑150 (MQ=60)
                                                                          GGCGGTCAGCGCCTTCGACACGGAATAAGAAGCCGTGCGCCTGGCCAACGACATCATCTCTGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACA                                                             <  NB501016:282:H2V7GAFX2:2:11211:26252:2897/149‑1 (MQ=60)
                                                                          GGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACAC                                                            >  NB501016:282:H2V7GAFX2:4:21601:6879:17211/1‑150 (MQ=60)
                                                                          GGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACAC                                                            <  NB501016:282:H2V7GAFX2:1:11106:11888:16632/150‑1 (MQ=60)
                                                                                      CTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTG                                               >  NB501016:282:H2V7GAFX2:3:21611:6373:19851/1‑151 (MQ=60)
                                                                                        TCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTG                                               <  NB501016:282:H2V7GAFX2:3:11403:12378:15101/149‑1 (MQ=60)
                                                                                           ACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAG                                                                                                 >  NB501016:282:H2V7GAFX2:2:21110:16238:4761/1‑96 (MQ=60)
                                                                                            CACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGAT                                            <  NB501016:282:H2V7GAFX2:4:21404:21976:2663/148‑1 (MQ=60)
                                                                                               GGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACG                                      >  NB501016:282:H2V7GAFX2:1:11109:19805:10520/1‑151 (MQ=60)
                                                                                                    AAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCC                                 >  NB501016:282:H2V7GAFX2:3:11502:13497:6831/1‑151 (MQ=60)
                                                                                                          CCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGG                           >  NB501016:282:H2V7GAFX2:1:11212:20180:18836/1‑151 (MQ=60)
                                                                                                                GCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTTCCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGG                     <  NB501016:282:H2V7GAFX2:4:11503:18666:7856/151‑1 (MQ=60)
                                                                                                                 CCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGC                    >  NB501016:282:H2V7GAFX2:1:21210:23034:12844/1‑151 (MQ=60)
                                                                                                                 CCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCAGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGC                    <  NB501016:282:H2V7GAFX2:1:11303:15548:11412/151‑1 (MQ=60)
                                                                                                                  CTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGC                    >  NB501016:282:H2V7GAFX2:3:21507:22926:15228/1‑150 (MQ=60)
                                                                                                                   TGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCA                   >  NB501016:282:H2V7GAFX2:2:11104:7015:6684/1‑150 (MQ=60)
                                                                                                                    GGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCA                   >  NB501016:282:H2V7GAFX2:2:21207:19515:12091/1‑149 (MQ=60)
                                                                                                                    GGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCA                   >  NB501016:282:H2V7GAFX2:1:21309:20666:2816/1‑149 (MQ=60)
                                                                                                                          CGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCG           >  NB501016:282:H2V7GAFX2:1:11206:5712:19607/1‑151 (MQ=60)
                                                                                                                           GACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGG          <  NB501016:282:H2V7GAFX2:2:11101:19418:3028/151‑1 (MQ=60)
                                                                                                                            ACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCTCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGG         <  NB501016:282:H2V7GAFX2:3:11509:18207:3823/151‑1 (MQ=60)
                                                                                                                            ACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGG         <  NB501016:282:H2V7GAFX2:4:11608:9734:9330/151‑1 (MQ=60)
                                                                                                                             CAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGG        <  NB501016:282:H2V7GAFX2:4:11408:18548:15117/151‑1 (MQ=60)
                                                                                                                                   CTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGC  >  NB501016:282:H2V7GAFX2:4:11509:19791:15828/1‑151 (MQ=60)
                                                                                                                                              |                                                                                                                                           
ATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGC  >  NC_002947_CJ‑RC/2958381‑2958662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑