Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F13 I0 R1 203 85.6 3649704 98.8% 3605907 146.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947_CJ‑RC 1,698,913 C→T 44.3% Q221* (CAG→TAG)  cheBC → chemotaxis response regulator protein‑glutamate methylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947_CJ‑RC1,698,9130CT44.3% 27.7 / 85.8 70Q221* (CAG→TAG) cheBCchemotaxis response regulator protein‑glutamate methylesterase
Reads supporting (aligned to +/- strand):  ref base C (18/21);  new base T (17/14);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-01

AGGGTTTGCCGGCAGCGTTTCCGGCGGCCATCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGTGATG  >  NC_002947_CJ‑RC/1698767‑1699057
                                                                                                                                                  |                                                                                                                                                
aGGGTTTGGCGGCAGCGTTTCCGGCGGCCATCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGAAGCGCTGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAg                                                                                                                                                <  2:413357/149‑1 (MQ=255)
aGGGTTTGCCGGCAGCGTTTCCGGCGGCCATCGTGCTGGTCCATCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAg                                                                                                                                                <  5:344579/149‑1 (MQ=255)
aGGGTTTGCCGGCAGCGTTTCCGGCGGCCATCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAg                                                                                                                                                <  6:162375/149‑1 (MQ=255)
aGGGTTTGCCGGCAGCGTTTCCGGCGGCCATCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAg                                                                                                                                                <  5:192479/149‑1 (MQ=255)
    tttGCCGGCAGCGTTTCCGGCGGCCATCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTAc                                                                                                                                            >  5:152765/1‑149 (MQ=255)
              gCGTTTCCGGCGGCCATCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCgg                                                                                                                                  >  3:85277/1‑149 (MQ=255)
               cGTTTCCGGCGGCCATCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGt                                                                                                                                 <  6:152765/149‑1 (MQ=255)
               cGTTTCCGGCGGCCATCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGt                                                                                                                                 <  4:59369/149‑1 (MQ=255)
                gTTTCCGGCGGCCATCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTa                                                                                                                                <  7:153632/149‑1 (MQ=255)
                        cggcCATCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCATGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAaccac                                                                                                                        <  1:189746/149‑1 (MQ=255)
                         ggcCATCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAaccacc                                                                                                                       >  8:179512/1‑149 (MQ=255)
                            cATCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACcaccac                                                                                                                     >  2:438718/1‑148 (MQ=255)
                             aTCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACAt                                                                                                                   >  3:48104/1‑149 (MQ=255)
                                 tGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGc                                                                                                               <  7:146030/149‑1 (MQ=255)
                                     ggTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGc                                                                                                           >  2:15205/1‑149 (MQ=255)
                                      gTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGc                                                                                                           >  1:147251/1‑148 (MQ=255)
                                      gTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGc                                                                                                           >  2:83842/1‑148 (MQ=255)
                                        ccAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTa                                                                                                         >  3:339766/1‑148 (MQ=255)
                                         cAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACa                                                                                                       <  6:146051/149‑1 (MQ=255)
                                          aGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTa                                                                                                         >  8:431988/1‑146 (MQ=255)
                                                       ggTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTgg                                                                                         >  6:278974/1‑149 (MQ=255)
                                                       ggTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTgg                                                                                         >  6:347314/1‑149 (MQ=255)
                                                        gtgtTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGc                                                                                        >  5:339135/1‑149 (MQ=255)
                                                         tgtTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGcc                                                                                       >  5:235811/1‑149 (MQ=255)
                                                           tttGCCGCAGGCATGGCCGATTGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTa                                                                                     >  7:276528/1‑149 (MQ=255)
                                                             tGCCGCAGGCATGGCCGAAAGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTaca                                                                                   >  3:202859/1‑149 (MQ=255)
                                                              gCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTacac                                                                                  >  7:243759/1‑149 (MQ=255)
                                                               ccGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACt                                                                                 >  7:200593/1‑149 (MQ=255)
                                                                cGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTg                                                                                >  7:238251/1‑149 (MQ=255)
                                                                cGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTg                                                                                >  4:201051/1‑149 (MQ=255)
                                                                  cAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGcc                                                                              <  1:444663/149‑1 (MQ=255)
                                                                    ggCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCg                                                                             >  6:67959/1‑148 (MQ=255)
                                                                       aTGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAAcc                                                                         <  7:179512/149‑1 (MQ=255)
                                                                        tGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCt                                                                        <  5:208082/149‑1 (MQ=255)
                                                                        tGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCt                                                                        >  5:155842/1‑149 (MQ=255)
                                                                          gCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCtgt                                                                      <  3:341059/149‑1 (MQ=255)
                                                                              aaTGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGtgaat                                                                  <  3:201051/149‑1 (MQ=255)
                                                                              aaTGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGtgaat                                                                  <  4:48104/149‑1 (MQ=255)
                                                                                    tCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATc                                                            >  8:177134/1‑149 (MQ=255)
                                                                                       gcagcgcagcAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTAc                                                         <  3:3497/149‑1 (MQ=255)
                                                                                       gcagcgcagcAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTAc                                                         <  1:308887/149‑1 (MQ=255)
                                                                                           cgcagcAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGc                                                     >  2:239988/1‑149 (MQ=255)
                                                                                            gcagcaGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGcc                                                    <  1:19498/149‑1 (MQ=255)
                                                                                              agcagGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCt                                                  >  6:260138/1‑149 (MQ=255)
                                                                                               gcagGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCtc                                                 >  5:309058/1‑149 (MQ=255)
                                                                                                 agGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCtcga                                               <  8:100419/149‑1 (MQ=255)
                                                                                                  ggCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCtcgat                                              >  4:152259/1‑149 (MQ=255)
                                                                                                   gCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCtcgatc                                             >  3:279592/1‑149 (MQ=255)
                                                                                                       gCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGAtg                                         >  3:102589/1‑149 (MQ=255)
                                                                                                        ccGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTcgatcgat                                          <  7:152980/147‑1 (MQ=255)
                                                                                                        ccGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTcgatcgat                                          <  5:323701/147‑1 (MQ=255)
                                                                                                               gcCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGttcttc                                 <  4:85277/149‑1 (MQ=255)
                                                                                                                 cTGGCCTGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCga                               <  3:125793/149‑1 (MQ=255)
                                                                                                                    gCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCgaga                             <  8:238251/148‑1 (MQ=255)
                                                                                                                     cccGTGAAGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGc                           <  6:339135/149‑1 (MQ=255)
                                                                                                                            aGGCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCAc                    <  7:431988/149‑1 (MQ=255)
                                                                                                                             ggCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACg                   >  4:131893/1‑149 (MQ=255)
                                                                                                                              gCGAACCGCCGCAGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGt                  >  5:354637/1‑149 (MQ=255)
                                                                                                                               cGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGtt                 <  3:131893/149‑1 (MQ=255)
                                                                                                                                gAACCGCCGCAGCCAGGGCACGTACTCCTGGCCGGTACCAACAACCACATTCGCCTGCTACAGAAACGCCAACTGGCCTACACTGCCGAACCTGAGAATGAAAACAACCGGCCCTCGATCGATGAGTACTTCGAGAGCCTGGCACGTTa                >  4:284424/1‑149 (MQ=255)
                                                                                                                                   ccgccgCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTg             >  2:185529/1‑149 (MQ=255)
                                                                                                                                      ccgcAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGCACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGaa          >  1:192915/1‑149 (MQ=255)
                                                                                                                                       cgcAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAAt         <  1:83842/149‑1 (MQ=255)
                                                                                                                                          aGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGt      <  4:102589/149‑1 (MQ=255)
                                                                                                                                          aGCCAGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGt      <  5:220946/149‑1 (MQ=255)
                                                                                                                                          aGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCtc                                                 <  8:102321/106‑1 (MQ=255)
                                                                                                                                          aGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCtc                                                 >  7:102321/1‑106 (MQ=255)
                                                                                                                                           gCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGTg     <  7:383631/149‑1 (MQ=255)
                                                                                                                                           gCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGTg     <  3:277676/149‑1 (MQ=255)
                                                                                                                                              aGGGTAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGTGATg  <  5:67959/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AGGGTTTGCCGGCAGCGTTTCCGGCGGCCATCGTGCTGGTCCAGCATGTGGACCAGGTGTTTGCCGCAGGCATGGCCGAATGGCTCAGCAGCGCAGCAGGCCTGCCGGTGCGCCTGGCCCGTGAAGGCGAACCGCCGCAGCCAGGGCAGGTACTCCTGGCCGGTACCAACCACCACATTCGCCTGCTACAGAATGGCCAACTGGCCTACACTGCCGAACCTGTGAATGAAATCTACCGGCCCTCGATCGATGTGTTCTTCGAGAGCGTGGCACGTTACTGGAATGGTGATG  >  NC_002947_CJ‑RC/1698767‑1699057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A