Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F14 I0 R1
|
248 |
61.6 |
2601574 |
98.7% |
2567753 |
146.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
1,842,575 |
Δ2 bp |
100% |
coding (2218‑2219/2754 nt) |
gacS ← |
sensor protein GacS |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 1,842,575 | 0 | T | . | 97.7%
| 193.5
/ ‑1.5
| 44 | coding (2219/2754 nt) | gacS | sensor protein GacS |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (26/17); total (26/18) |
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
| * | NC_002947_CJ‑RC | 1,842,576 | 0 | G | . | 97.7%
| 190.1
/ ‑2.0
| 44 | coding (2218/2754 nt) | gacS | sensor protein GacS |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (26/17); total (26/18) |
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. |
CACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGC > NC_002947_CJ‑RC/1842434‑1842708
||
cACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTtct > 6:111295/1‑149 (MQ=255)
cTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTcc < 6:159686/149‑1 (MQ=255)
atcaCCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCca > 8:42450/1‑147 (MQ=255)
accaccTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGc < 7:233818/149‑1 (MQ=255)
accTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGa > 6:282066/1‑149 (MQ=255)
gCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGtt < 4:58217/149‑1 (MQ=255)
cAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCg < 6:315024/149‑1 (MQ=255)
gTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCgg > 5:33151/1‑148 (MQ=255)
ttGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCCGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTa < 4:89183/149‑1 (MQ=255)
gCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTa > 1:40974/1‑147 (MQ=255)
ttCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGgcg > 6:127184/1‑149 (MQ=255)
tGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGcc > 4:201755/1‑149 (MQ=255)
tGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGcc > 4:100922/1‑149 (MQ=255)
gTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCgg > 5:14754/1‑149 (MQ=255)
tCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCg < 4:186627/149‑1 (MQ=255)
gTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATc > 8:117895/1‑149 (MQ=255)
gtcgtcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTg > 4:202639/1‑148 (MQ=255)
gtcgtcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGc < 5:137116/149‑1 (MQ=255)
tcgtcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGca < 5:220811/149‑1 (MQ=255)
tcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCTGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCAcat < 2:140797/149‑1 (MQ=255)
tGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCAcatccat < 3:100922/149‑1 (MQ=255)
gCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCAGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCAcatccat > 3:247330/1‑148 (MQ=255)
cTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCa > 4:138391/1‑149 (MQ=255)
tgCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCa < 5:214143/149‑1 (MQ=255)
caacaaCGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAgg > 7:139533/1‑149 (MQ=255)
aacaacGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGt > 3:276531/1‑149 (MQ=255)
aacaacGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGt > 3:233057/1‑149 (MQ=255)
gcgcgTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGTGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAAc > 5:80014/1‑148 (MQ=255)
gcgcgTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAAc > 7:332931/1‑148 (MQ=255)
gcgTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAAt > 4:66195/1‑149 (MQ=255)
cgTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAAtt < 7:42450/149‑1 (MQ=255)
tttttCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAAttct < 4:233057/149‑1 (MQ=255)
tttCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAAttcttc > 4:124210/1‑149 (MQ=255)
ttCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTcc > 6:38878/1‑149 (MQ=255)
ttCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTcc > 4:70746/1‑149 (MQ=255)
tCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTcc < 4:183588/148‑1 (MQ=255)
gTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGc < 7:18120/149‑1 (MQ=255)
atggcatggGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCt > 1:114317/1‑149 (MQ=255)
atggcatggGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCt < 5:111295/149‑1 (MQ=255)
atggGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTAcc > 7:292265/1‑149 (MQ=255)
gggCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAAc > 7:216865/1‑148 (MQ=255)
acgaTCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCAttgt < 5:282066/149‑1 (MQ=255)
acgaTCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCAttgt < 8:139533/149‑1 (MQ=255)
cAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGc > 1:270996/1‑148 (MQ=255)
||
CACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGC > NC_002947_CJ‑RC/1842434‑1842708
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCC > NC_002947_CJ‑RC/1842442‑1842709
|
CACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCG < NB501016:282:H2V7GAFX2:4:21502:4852:11374/151‑1 (MQ=60)
AGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGT > NB501016:282:H2V7GAFX2:3:11408:15586:9092/1‑150 (MQ=60)
TGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTAC > NB501016:282:H2V7GAFX2:1:11109:11516:7429/1‑149 (MQ=60)
GGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGG > NB501016:282:H2V7GAFX2:3:11404:19273:6184/1‑151 (MQ=60)
AGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCA < NB501016:282:H2V7GAFX2:3:11606:5869:12748/151‑1 (MQ=60)
GTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCAC < NB501016:282:H2V7GAFX2:3:21501:13810:12179/151‑1 (MQ=60)
ATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATC < NB501016:282:H2V7GAFX2:2:11211:19237:13048/151‑1 (MQ=60)
TGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCAGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATC > NB501016:282:H2V7GAFX2:2:21210:26078:6462/1‑150 (MQ=60)
GTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAG < NB501016:282:H2V7GAFX2:3:21412:3512:2405/151‑1 (MQ=60)
GCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGT > NB501016:282:H2V7GAFX2:4:11606:8135:12607/1‑151 (MQ=60)
CAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTC > NB501016:282:H2V7GAFX2:2:21207:11077:3319/1‑151 (MQ=60)
CAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTC > NB501016:282:H2V7GAFX2:2:21305:9035:9316/1‑151 (MQ=60)
CGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGTGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACA > NB501016:282:H2V7GAFX2:3:11506:12841:13706/1‑150 (MQ=60)
CGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACA > NB501016:282:H2V7GAFX2:4:21612:2968:6827/1‑150 (MQ=60)
GCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTC < NB501016:282:H2V7GAFX2:4:11409:6958:16290/151‑1 (MQ=60)
CGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCA < NB501016:282:H2V7GAFX2:4:11404:17483:16780/151‑1 (MQ=60)
CATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTG < NB501016:282:H2V7GAFX2:3:11601:16269:7783/151‑1 (MQ=60)
CATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTG > NB501016:282:H2V7GAFX2:1:11301:21662:1542/1‑151 (MQ=60)
CATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCG > NB501016:282:H2V7GAFX2:4:21603:12302:3593/1‑151 (MQ=60)
AGGGCGACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACC > NB501016:282:H2V7GAFX2:4:21411:25075:1465/1‑150 (MQ=60)
GACGATCGGCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTT < NB501016:282:H2V7GAFX2:3:21603:6309:4189/151‑1 (MQ=60)
GCAGCGGGTTGCCGCTC‑‑GGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCC > NB501016:282:H2V7GAFX2:1:21211:23220:13004/1‑150 (MQ=60)
|
CACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCC > NC_002947_CJ‑RC/1842442‑1842709
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
