Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F24 I1 R1 57 68.7 2934938 98.3% 2885044 146.2

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NC_002947_CJ‑RC/4348662‑4348961
                                                                                                                                                      |                                                                                                                                                     
GGGATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCA                                                                                                                                                       <  NB501016:282:H2V7GAFX2:1:11211:16040:14277/151‑1 (MQ=0)
  GATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTGATC                                                                                                                                                     <  NB501016:282:H2V7GAFX2:4:21511:13172:1175/151‑1 (MQ=0)
        GGATGACAGTTTCCGCTGTGGGAAATTTCCGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCG                                                                                                                                               >  NB501016:282:H2V7GAFX2:1:21107:8632:9251/1‑151 (MQ=0)
          ATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCC                                                                                                                                             >  NB501016:282:H2V7GAFX2:4:21610:5828:6583/1‑151 (MQ=0)
             ACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTT                                                                                                                                          <  NB501016:282:H2V7GAFX2:3:21602:5655:15070/151‑1 (MQ=0)
             ACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTT                                                                                                                                          >  NB501016:282:H2V7GAFX2:3:21605:10619:13008/1‑151 (MQ=0)
               AGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCG                                                                                                                                        <  NB501016:282:H2V7GAFX2:3:21506:16916:18197/151‑1 (MQ=0)
                   TCCGCTGTGTGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACG                                                                                                                                     <  NB501016:282:H2V7GAFX2:3:21407:4983:9569/150‑1 (MQ=0)
                      GCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGTATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCG                                                                                                                                 <  NB501016:282:H2V7GAFX2:1:11304:1254:10558/151‑1 (MQ=0)
                         GTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGG                                                                                                                                <  NB501016:282:H2V7GAFX2:2:21105:1755:4740/149‑1 (MQ=0)
                            GGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGC                                                                                                                            >  NB501016:282:H2V7GAFX2:2:11311:12540:3060/1‑150 (MQ=0)
                             GAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTTGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTAGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCA                                                                                                                           >  NB501016:282:H2V7GAFX2:3:11605:15494:1805/1‑150 (MQ=0)
                               AATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAG                                                                                                                        <  NB501016:282:H2V7GAFX2:1:21206:3470:11282/151‑1 (MQ=0)
                                ATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGT                                                                                                                       <  NB501016:282:H2V7GAFX2:4:11504:5610:14129/151‑1 (MQ=0)
                                ATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGT                                                                                                                       >  NB501016:282:H2V7GAFX2:4:21602:16058:8423/1‑151 (MQ=0)
                                  TTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTG                                                                                                                      <  NB501016:282:H2V7GAFX2:2:21107:18653:9212/150‑1 (MQ=0)
                                   TCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGG                                                                                                                     <  NB501016:282:H2V7GAFX2:3:21503:1598:9801/150‑1 (MQ=0)
                                      TCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC                                                                                                                    <  NB501016:282:H2V7GAFX2:4:21505:4901:2201/148‑1 (MQ=1)
                                           ACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                            <  NB501016:282:H2V7GAFX2:1:21211:14884:5526/151‑1 (MQ=21)
                                           ACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                            <  NB501016:282:H2V7GAFX2:4:11607:20289:14021/151‑1 (MQ=21)
                                           ACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                            >  NB501016:282:H2V7GAFX2:1:21108:7881:11840/1‑151 (MQ=21)
                                           ACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                            <  NB501016:282:H2V7GAFX2:3:11407:20477:12543/151‑1 (MQ=21)
                                             GGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAG                                                                                                          <  NB501016:282:H2V7GAFX2:1:21112:18473:2914/151‑1 (MQ=22)
                                              GTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCGAGCGTAC                                                                                                            >  NB501016:282:H2V7GAFX2:4:11407:6644:14246/1‑148 (MQ=16)
                                                 ACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCT                                                                                                      >  NB501016:282:H2V7GAFX2:1:21108:24768:19687/1‑151 (MQ=21)
                                                          ACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGT                                                                                              >  NB501016:282:H2V7GAFX2:3:21405:3231:18575/1‑150 (MQ=22)
                                                           CGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTA                                                                                             >  NB501016:282:H2V7GAFX2:1:11106:20325:12439/1‑150 (MQ=22)
                                                                ACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGC                                                                                       <  NB501016:282:H2V7GAFX2:3:11504:3267:7719/151‑1 (MQ=22)
                                                                       CTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCT                                                                                >  NB501016:282:H2V7GAFX2:2:21107:3440:12651/1‑151 (MQ=21)
                                                                          GCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCC                                                                             <  NB501016:282:H2V7GAFX2:1:11104:7925:18839/151‑1 (MQ=21)
                                                                           CGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCT                                                                            >  NB501016:282:H2V7GAFX2:3:21607:2224:6280/1‑151 (MQ=21)
                                                                           CGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCT                                                                            <  NB501016:282:H2V7GAFX2:2:21305:6099:14084/151‑1 (MQ=22)
                                                                              CATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCT                                                                         <  NB501016:282:H2V7GAFX2:1:11310:17928:19356/151‑1 (MQ=22)
                                                                                TAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTT                                                                        >  NB501016:282:H2V7GAFX2:3:11603:22239:3589/1‑150 (MQ=22)
                                                                                 AACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTAT                                                                      >  NB501016:282:H2V7GAFX2:3:21601:5400:19324/1‑151 (MQ=21)
                                                                                   CCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTA                                                                       <  NB501016:282:H2V7GAFX2:3:11610:12567:6050/148‑1 (MQ=21)
                                                                                   CCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCT                                                                         <  NB501016:282:H2V7GAFX2:3:11403:8996:7462/146‑1 (MQ=21)
                                                                                   CCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGC                                                                    >  NB501016:282:H2V7GAFX2:2:21303:18657:20211/1‑151 (MQ=22)
                                                                                     TGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCT                                                                  >  NB501016:282:H2V7GAFX2:3:11410:8210:8939/1‑151 (MQ=22)
                                                                                           GCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGA                                                            <  NB501016:282:H2V7GAFX2:4:11606:8860:9918/151‑1 (MQ=21)
                                                                                            CCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGAT                                                           <  NB501016:282:H2V7GAFX2:4:11602:17776:16556/151‑1 (MQ=22)
                                                                                            CCCTTGGTGGCGCGGTAAACCGCCACTTACTACGGCTTCGGCTGACATCTGCACGCTCATCCCGTCGCCTATCGACGCGCGGTAGCACACTGGCAAGCGTACACAACTCCCAGCGTAATTCGCGCGACCATCCTGCTTATGCCTGTCGGA                                                            >  NB501016:282:H2V7GAFX2:2:21110:2010:17877/1‑150 (MQ=0)
                                                                                                    GGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTC                                                    >  NB501016:282:H2V7GAFX2:3:11609:16825:1834/1‑150 (MQ=21)
                                                                                                     GCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCAC                                                  <  NB501016:282:H2V7GAFX2:1:11311:6963:12349/151‑1 (MQ=22)
                                                                                                       GCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTTCGTCACAG                                                <  NB501016:282:H2V7GAFX2:4:21603:1586:19524/151‑1 (MQ=20)
                                                                                                        CGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGC                                               >  NB501016:282:H2V7GAFX2:2:21104:3660:9806/1‑151 (MQ=21)
                                                                                                              ACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCG                                         >  NB501016:282:H2V7GAFX2:2:21105:8027:7746/1‑151 (MQ=21)
                                                                                                               CCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCATACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGT                                        >  NB501016:282:H2V7GAFX2:4:21409:5847:1621/1‑151 (MQ=20)
                                                                                                                  CCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGTCGCGCGGTAGGACAGTGGCAAGCGTACAGATCTCCCAGTGTAATTCGCGCGTCCTTCCTGCTTATGCCTGTCGGATCTACGTCTCAGCGTTCCGTGCA                                     <  NB501016:282:H2V7GAFX2:1:21210:26486:12434/151‑1 (MQ=19)
                                                                                                                    ACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCA                                     >  NB501016:282:H2V7GAFX2:3:21411:13742:18402/1‑149 (MQ=21)
                                                                                                                        ACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTAT                                <  NB501016:282:H2V7GAFX2:1:21201:22343:14247/150‑1 (MQ=21)
                                                                                                                           ACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGG                            <  NB501016:282:H2V7GAFX2:1:21101:19546:13713/151‑1 (MQ=21)
                                                                                                                           ACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGG                            <  NB501016:282:H2V7GAFX2:4:21511:10089:17470/151‑1 (MQ=22)
                                                                                                                                  CGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGG                      <  NB501016:282:H2V7GAFX2:4:11610:8487:9272/150‑1 (MQ=21)
                                                                                                                                    GCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCG                    >  NB501016:282:H2V7GAFX2:2:21302:24948:2999/1‑150 (MQ=22)
                                                                                                                                        ACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTG               <  NB501016:282:H2V7GAFX2:1:21308:4277:5749/151‑1 (MQ=22)
                                                                                                                                        ACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTG               <  NB501016:282:H2V7GAFX2:2:21108:10701:2605/151‑1 (MQ=22)
                                                                                                                                          TTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTG             >  NB501016:282:H2V7GAFX2:2:11108:18507:6932/1‑151 (MQ=22)
                                                                                                                                           TCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTG             <  NB501016:282:H2V7GAFX2:3:11407:2640:3651/150‑1 (MQ=22)
                                                                                                                                              GCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCA          <  NB501016:282:H2V7GAFX2:3:21608:22509:3242/150‑1 (MQ=22)
                                                                                                                                              GCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCC           <  NB501016:282:H2V7GAFX2:4:21610:22794:13384/149‑1 (MQ=22)
                                                                                                                                               CACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACC        <  NB501016:282:H2V7GAFX2:3:11501:14992:2222/151‑1 (MQ=21)
                                                                                                                                                     CATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NB501016:282:H2V7GAFX2:3:11502:8945:9550/1‑151 (MQ=22)
                                                                                                                                                      |                                                                                                                                                     
GGGATTCGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NC_002947_CJ‑RC/4348662‑4348961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑