Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F43 I1 R1 57 79.1 3360134 98.7% 3316452 147.0

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCCC  >  NC_002947_CJ‑RC/4348670‑4348962
                                                                                                                                              |                                                                                                                                                      
GGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCG                                                                                                                                                >  NB501016:282:H2V7GAFX2:2:21207:9158:19742/1‑151 (MQ=0)
   TGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCT                                                                                                                                             >  NB501016:282:H2V7GAFX2:3:11611:18886:11506/1‑151 (MQ=0)
       AGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCG                                                                                                                                         <  NB501016:282:H2V7GAFX2:4:21407:26662:11996/151‑1 (MQ=0)
        GTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCG                                                                                                                                         >  NB501016:282:H2V7GAFX2:3:21504:4343:6912/1‑150 (MQ=0)
                 GTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGT                                                                                                                                >  NB501016:282:H2V7GAFX2:3:21609:21844:5970/1‑150 (MQ=0)
                   GGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGC                                                                                                                             >  NB501016:282:H2V7GAFX2:3:11411:18494:13341/1‑151 (MQ=0)
                              GCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAG                                                                                                                  <  NB501016:282:H2V7GAFX2:3:11504:11725:9458/151‑1 (MQ=4)
                               CATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGC                                                                                                                 >  NB501016:282:H2V7GAFX2:3:11412:20918:7838/1‑151 (MQ=6)
                                ATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCG                                                                                                                <  NB501016:282:H2V7GAFX2:4:21501:22197:13394/151‑1 (MQ=17)
                                ATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCG                                                                                                                >  NB501016:282:H2V7GAFX2:4:21512:6039:4106/1‑151 (MQ=17)
                                ATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCACTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCG                                                                                                                <  NB501016:282:H2V7GAFX2:4:11504:3057:20070/151‑1 (MQ=16)
                                  TACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGT                                                                                                               <  NB501016:282:H2V7GAFX2:1:21101:17645:8618/150‑1 (MQ=19)
                                   ACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                             <  NB501016:282:H2V7GAFX2:4:11512:24091:12096/151‑1 (MQ=22)
                                     GGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAG                                                                                                           <  NB501016:282:H2V7GAFX2:4:11407:14239:1551/151‑1 (MQ=21)
                                            AACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCC                                                                                                    >  NB501016:282:H2V7GAFX2:4:21512:14205:6224/1‑151 (MQ=22)
                                                         CGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGAGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCG                                                                                       >  NB501016:282:H2V7GAFX2:4:11401:25598:1669/1‑151 (MQ=21)
                                                           CCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCG                                                                                     >  NB501016:282:H2V7GAFX2:4:11405:22827:10998/1‑151 (MQ=21)
                                                           CCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCG                                                                                     <  NB501016:282:H2V7GAFX2:1:21103:3293:18735/151‑1 (MQ=22)
                                                              ACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGA                                                                                    <  NB501016:282:H2V7GAFX2:1:21203:6792:17744/149‑1 (MQ=21)
                                                               CTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCT                                                                                 >  NB501016:282:H2V7GAFX2:3:11606:18534:6348/1‑151 (MQ=22)
                                                                  GCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCC                                                                              <  NB501016:282:H2V7GAFX2:1:11106:6606:2592/151‑1 (MQ=21)
                                                                    GGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTG                                                                            <  NB501016:282:H2V7GAFX2:3:21412:3932:3876/151‑1 (MQ=21)
                                                                      AATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCT                                                                          <  NB501016:282:H2V7GAFX2:1:11302:10604:13693/151‑1 (MQ=21)
                                                                        TAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTT                                                                         >  NB501016:282:H2V7GAFX2:1:11101:2794:17384/1‑150 (MQ=21)
                                                                        TAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTT                                                                         >  NB501016:282:H2V7GAFX2:1:21109:5291:15583/1‑150 (MQ=21)
                                                                         AACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTAT                                                                       <  NB501016:282:H2V7GAFX2:1:21309:20738:3371/151‑1 (MQ=22)
                                                                         AACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTAT                                                                       <  NB501016:282:H2V7GAFX2:1:21210:5029:7921/151‑1 (MQ=22)
                                                                          ACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATG                                                                      <  NB501016:282:H2V7GAFX2:4:11408:11140:17819/151‑1 (MQ=21)
                                                                          ACCTGATCGGCCCTTGGTGGCGCGGTAAACCGCCACTTACTACGGCTACGGCTGACTTCTGCACGCTCGTCCCGTCGCCATTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAAATC                                                                                          >  NB501016:282:H2V7GAFX2:2:21110:25616:14721/1‑131 (MQ=19)
                                                                                 CGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCG                                                               >  NB501016:282:H2V7GAFX2:4:21502:23062:10936/1‑151 (MQ=21)
                                                                                    CCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGAT                                                            >  NB501016:282:H2V7GAFX2:3:21510:3648:14901/1‑151 (MQ=22)
                                                                                      CTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCT                                                          >  NB501016:282:H2V7GAFX2:4:11403:19194:19866/1‑151 (MQ=21)
                                                                                         GGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACG                                                       <  NB501016:282:H2V7GAFX2:2:21308:17331:15657/151‑1 (MQ=21)
                                                                                         GGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACG                                                       <  NB501016:282:H2V7GAFX2:2:11108:3462:9940/151‑1 (MQ=22)
                                                                                               GCGGTTAACCGCCACTTACTACTGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAG                                                 <  NB501016:282:H2V7GAFX2:4:11511:3378:16913/151‑1 (MQ=20)
                                                                                               GCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCAGTCGGATCTACGTCACAG                                                 <  NB501016:282:H2V7GAFX2:1:11208:5576:8673/151‑1 (MQ=20)
                                                                                                      ACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCG                                          >  NB501016:282:H2V7GAFX2:2:21207:11676:4509/1‑151 (MQ=22)
                                                                                                        CGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTG                                        <  NB501016:282:H2V7GAFX2:1:21103:19723:20118/151‑1 (MQ=21)
                                                                                                            ACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAG                                    <  NB501016:282:H2V7GAFX2:3:11512:16547:14594/151‑1 (MQ=22)
                                                                                                                   ACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGG                             <  NB501016:282:H2V7GAFX2:2:21212:9526:19093/151‑1 (MQ=21)
                                                                                                                    CGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGC                            <  NB501016:282:H2V7GAFX2:1:11101:6562:9814/151‑1 (MQ=21)
                                                                                                                         TCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTG                        <  NB501016:282:H2V7GAFX2:4:21412:20009:7605/150‑1 (MQ=21)
                                                                                                                          CGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGG                       <  NB501016:282:H2V7GAFX2:1:11104:16020:11410/150‑1 (MQ=21)
                                                                                                                          CGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGC                      <  NB501016:282:H2V7GAFX2:4:11502:11239:7812/151‑1 (MQ=22)
                                                                                                                            GCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGA                    <  NB501016:282:H2V7GAFX2:3:11401:2818:16539/151‑1 (MQ=21)
                                                                                                                            GCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCG                     >  NB501016:282:H2V7GAFX2:1:21201:6559:19072/1‑150 (MQ=21)
                                                                                                                               GACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTT                 <  NB501016:282:H2V7GAFX2:4:11606:11763:8433/151‑1 (MQ=22)
                                                                                                                               GACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTT                 >  NB501016:282:H2V7GAFX2:2:11203:25998:8456/1‑151 (MQ=22)
                                                                                                                                 CTTCTGCACGCTCGTCCCGTCGCCTTACGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCGGTGCAAGTATTGGGCTTTGGCGATTTGT               >  NB501016:282:H2V7GAFX2:4:21412:14684:1767/1‑151 (MQ=20)
                                                                                                                                   TCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGC             >  NB501016:282:H2V7GAFX2:4:21604:23385:12251/1‑151 (MQ=21)
                                                                                                                                   TCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTG              <  NB501016:282:H2V7GAFX2:1:21206:11810:2700/150‑1 (MQ=22)
                                                                                                                                      GCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCC            <  NB501016:282:H2V7GAFX2:4:21605:26096:15122/149‑1 (MQ=21)
                                                                                                                                           CTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATT‑GGCTTTGGCGATTTGTGCCACCTCACC    >  NB501016:282:H2V7GAFX2:3:21504:11296:4442/1‑151 (MQ=20)
                                                                                                                                              ATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCCC  >  NB501016:282:H2V7GAFX2:4:21604:14802:1159/1‑151 (MQ=22)
                                                                                                                                              |                                                                                                                                                      
GGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCCC  >  NC_002947_CJ‑RC/4348670‑4348962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑