Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F49 I1 R1 57 62.9 2678826 98.4% 2635964 146.3

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAC  >  NC_002947_CJ‑RC/4348672‑4348954
                                                                                                                                            |                                                                                                                                              
ATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCC                                                                                                                                      <  NB501016:282:H2V7GAFX2:3:11407:6581:3799/151‑1 (MQ=0)
    CAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTC                                                                                                                                  >  NB501016:282:H2V7GAFX2:1:21311:18429:5076/1‑151 (MQ=0)
          CCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGC                                                                                                                             <  NB501016:282:H2V7GAFX2:3:21410:16641:13294/150‑1 (MQ=0)
            GCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCG                                                                                                                          >  NB501016:282:H2V7GAFX2:2:21304:2029:14350/1‑151 (MQ=0)
                 GGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGTCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAG                                                                                                                      <  NB501016:282:H2V7GAFX2:1:21103:3152:5661/150‑1 (MQ=0)
                 GGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAG                                                                                                                      <  NB501016:282:H2V7GAFX2:3:11410:12487:19071/150‑1 (MQ=0)
                   GAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCAC                                                                                                                   <  NB501016:282:H2V7GAFX2:1:21210:10710:8558/151‑1 (MQ=0)
                         TCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGG                                                                                                              <  NB501016:282:H2V7GAFX2:1:11302:15086:14781/150‑1 (MQ=0)
                          CTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC                                                                                                             >  NB501016:282:H2V7GAFX2:1:21111:11616:9894/1‑150 (MQ=2)
                             CATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGC                                                                                                         <  NB501016:282:H2V7GAFX2:4:11501:6653:3700/151‑1 (MQ=6)
                             CATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGACGCCTTTCGACGCGCGGTAGCACAGTGGCAAGC                                                                                                         >  NB501016:282:H2V7GAFX2:3:21610:22785:11497/1‑151 (MQ=6)
                                  CGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                     >  NB501016:282:H2V7GAFX2:4:21410:3335:12639/1‑150 (MQ=21)
                                   GGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAG                                                                                                   <  NB501016:282:H2V7GAFX2:3:11505:2959:13538/151‑1 (MQ=21)
                                    GTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGA                                                                                                  <  NB501016:282:H2V7GAFX2:3:11603:24988:13769/151‑1 (MQ=21)
                                      TACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                     >  NB501016:282:H2V7GAFX2:4:21510:16202:10860/1‑146 (MQ=22)
                                            CGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAG                                                                                          <  NB501016:282:H2V7GAFX2:3:11408:11096:9071/151‑1 (MQ=21)
                                            CGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAG                                                                                          <  NB501016:282:H2V7GAFX2:1:21211:23744:15295/151‑1 (MQ=22)
                                              AGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGG                                                                                        <  NB501016:282:H2V7GAFX2:3:11510:8791:3487/151‑1 (MQ=22)
                                              AGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGG                                                                                        <  NB501016:282:H2V7GAFX2:3:11502:6084:9666/151‑1 (MQ=22)
                                                       CGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCG                                                                               <  NB501016:282:H2V7GAFX2:2:21303:15303:7165/151‑1 (MQ=22)
                                                       CGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCG                                                                               <  NB501016:282:H2V7GAFX2:1:21103:15181:12529/151‑1 (MQ=22)
                                                         CCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCG                                                                             >  NB501016:282:H2V7GAFX2:4:11403:9985:9728/1‑151 (MQ=21)
                                                         CCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAACACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCG                                                                             >  NB501016:282:H2V7GAFX2:3:11612:15529:19977/1‑151 (MQ=20)
                                                           TACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGAC                                                                           >  NB501016:282:H2V7GAFX2:4:21510:14864:18651/1‑151 (MQ=21)
                                                           TACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATC                                                                                                >  NB501016:282:H2V7GAFX2:3:11504:21296:11769/1‑130 (MQ=22)
                                                                  GGCATAACCTGTTCGGCCCTTGATGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTG                                                                    <  NB501016:282:H2V7GAFX2:4:21505:14390:8105/151‑1 (MQ=21)
                                                                    CATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCT                                                                  >  NB501016:282:H2V7GAFX2:3:21604:21880:9348/1‑151 (MQ=21)
                                                                    CATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCT                                                                  <  NB501016:282:H2V7GAFX2:3:11412:26203:9138/151‑1 (MQ=22)
                                                                            GTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCC                                                            <  NB501016:282:H2V7GAFX2:2:21312:15003:15165/149‑1 (MQ=21)
                                                                            GTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCC                                                            <  NB501016:282:H2V7GAFX2:1:21109:14354:2924/149‑1 (MQ=21)
                                                                            GTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCC                                                            <  NB501016:282:H2V7GAFX2:1:21111:3379:19540/149‑1 (MQ=22)
                                                                               CGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCG                                                       >  NB501016:282:H2V7GAFX2:4:11408:4119:7026/1‑151 (MQ=22)
                                                                                GGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGG                                                      >  NB501016:282:H2V7GAFX2:3:21605:9239:5631/1‑151 (MQ=21)
                                                                                   CCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATC                                                   >  NB501016:282:H2V7GAFX2:4:21410:5234:12087/1‑151 (MQ=21)
                                                                                   CCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATC                                                   <  NB501016:282:H2V7GAFX2:4:11509:8294:8238/151‑1 (MQ=22)
                                                                                      TGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCT                                                  <  NB501016:282:H2V7GAFX2:2:11305:14565:18287/149‑1 (MQ=22)
                                                                                            CGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCAC                                           >  NB501016:282:H2V7GAFX2:4:11609:14462:8009/1‑150 (MQ=21)
                                                                                             GCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAG                                         <  NB501016:282:H2V7GAFX2:4:21607:4793:11227/151‑1 (MQ=21)
                                                                                                   AACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCC                                   <  NB501016:282:H2V7GAFX2:2:21311:11863:2860/151‑1 (MQ=22)
                                                                                                     CCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGT                                 >  NB501016:282:H2V7GAFX2:3:21503:6429:7915/1‑151 (MQ=21)
                                                                                                           CTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGT                           >  NB501016:282:H2V7GAFX2:2:11302:22004:5762/1‑151 (MQ=22)
                                                                                                               CTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTG                       >  NB501016:282:H2V7GAFX2:3:11507:4983:11841/1‑151 (MQ=21)
                                                                                                               CTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTA                                                                                      >  NB501016:282:H2V7GAFX2:2:21109:18008:8284/1‑88 (MQ=26)
                                                                                                               CTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTG                       >  NB501016:282:H2V7GAFX2:3:11601:23855:17887/1‑151 (MQ=22)
                                                                                                                TACGGCTTCGGCTGACTTCTGCACGCTCATCCATTCGCCTTTCGCCGCGCGGTAGAACAGTCGCAAGCGTACAGATCTCACAGGGTAATTCGCGGGACCTTCCTGCTTATGACTGTCGGATCTACGTCACCGCGTTCCGTGCATGTATTGG                      >  NB501016:282:H2V7GAFX2:4:21604:3276:4426/1‑151 (MQ=16)
                                                                                                                 ACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGG                     <  NB501016:282:H2V7GAFX2:4:21403:8061:4080/151‑1 (MQ=21)
                                                                                                                   GGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCT                   >  NB501016:282:H2V7GAFX2:3:21506:7888:1800/1‑151 (MQ=22)
                                                                                                                    GCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTT                  <  NB501016:282:H2V7GAFX2:2:11208:1315:5605/151‑1 (MQ=21)
                                                                                                                    GCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTT                  <  NB501016:282:H2V7GAFX2:2:11209:26826:5596/151‑1 (MQ=21)
                                                                                                                    GCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTT                  <  NB501016:282:H2V7GAFX2:2:11212:11751:12801/151‑1 (MQ=21)
                                                                                                                     CATCGGCTGACTTCTGCAAGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTAGCGCGACCTTCCTGCTTAAGCCAGTCGGATCTACGACACAGCGTTCCCTGCAAGTATTGGGCTTT                 >  NB501016:282:H2V7GAFX2:1:11312:25927:13993/1‑151 (MQ=18)
                                                                                                                        CGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGC              <  NB501016:282:H2V7GAFX2:2:11210:11693:2830/151‑1 (MQ=21)
                                                                                                                        CGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCATGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGC              <  NB501016:282:H2V7GAFX2:2:21203:21644:11602/151‑1 (MQ=21)
                                                                                                                        CGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGC              <  NB501016:282:H2V7GAFX2:3:11504:7063:17491/151‑1 (MQ=22)
                                                                                                                        CGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGC              <  NB501016:282:H2V7GAFX2:2:11307:21356:1986/151‑1 (MQ=22)
                                                                                                                        CGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGG               <  NB501016:282:H2V7GAFX2:3:11508:8767:15204/150‑1 (MQ=22)
                                                                                                                         GGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCG             >  NB501016:282:H2V7GAFX2:4:11511:10510:19327/1‑151 (MQ=21)
                                                                                                                         GGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGG                     <  NB501016:282:H2V7GAFX2:4:21403:3766:14548/143‑1 (MQ=22)
                                                                                                                              ACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTG        <  NB501016:282:H2V7GAFX2:1:21104:18797:12076/151‑1 (MQ=21)
                                                                                                                               CTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGT       >  NB501016:282:H2V7GAFX2:1:11104:16327:1673/1‑151 (MQ=21)
                                                                                                                               CTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGT       >  NB501016:282:H2V7GAFX2:3:21507:16137:7248/1‑151 (MQ=21)
                                                                                                                               CTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATAGGGCTTTGGCGATTTGT       >  NB501016:282:H2V7GAFX2:1:21208:21742:5693/1‑151 (MQ=20)
                                                                                                                                    GCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAC  >  NB501016:282:H2V7GAFX2:1:21211:6542:2661/1‑151 (MQ=22)
                                                                                                                                            |                                                                                                                                              
ATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAC  >  NC_002947_CJ‑RC/4348672‑4348954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑