Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 54 84.9 3616994 98.4% 3559122 146.8

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NC_002947_CJ‑RC/4348668‑4348961
                                                                                                                                                |                                                                                                                                                     
CGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCG                                                                                                                                                  <  NB501016:282:H2V7GAFX2:1:11102:15102:15100/150‑1 (MQ=0)
CGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCG                                                                                                                                                  <  NB501016:282:H2V7GAFX2:4:21510:20923:2879/150‑1 (MQ=0)
     TGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCT                                                                                                                                            >  NB501016:282:H2V7GAFX2:4:11604:19650:17567/1‑151 (MQ=0)
        CAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTC                                                                                                                                         <  NB501016:282:H2V7GAFX2:2:11201:6478:15170/151‑1 (MQ=0)
          GTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGA                                                                                                                                       <  NB501016:282:H2V7GAFX2:1:21201:10546:12877/151‑1 (MQ=0)
             TCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACG                                                                                                                                     <  NB501016:282:H2V7GAFX2:4:21604:16959:13755/150‑1 (MQ=0)
                GCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCG                                                                                                                                 >  NB501016:282:H2V7GAFX2:4:11603:10508:3298/1‑151 (MQ=0)
                   GTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGT                                                                                                                               >  NB501016:282:H2V7GAFX2:4:11606:6892:13020/1‑150 (MQ=0)
                     GGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGC                                                                                                                            >  NB501016:282:H2V7GAFX2:4:11505:17481:9318/1‑151 (MQ=0)
                            TTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTG                                                                                                                      <  NB501016:282:H2V7GAFX2:2:21108:12380:5266/150‑1 (MQ=0)
                                GCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC                                                                                                                    <  NB501016:282:H2V7GAFX2:3:21404:4396:6062/148‑1 (MQ=2)
                                GCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAG                                                                                                                 <  NB501016:282:H2V7GAFX2:4:21606:8209:16249/151‑1 (MQ=4)
                                GCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGC                                                                                                                    <  NB501016:282:H2V7GAFX2:2:11206:21133:14040/148‑1 (MQ=2)
                                  ATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCG                                                                                                               <  NB501016:282:H2V7GAFX2:4:11503:20049:15258/151‑1 (MQ=17)
                                     ACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                            <  NB501016:282:H2V7GAFX2:1:11305:15990:5575/151‑1 (MQ=21)
                                        GTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGA                                                                                                         <  NB501016:282:H2V7GAFX2:2:21311:19819:12697/151‑1 (MQ=21)
                                         TTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTAC                                                                                                            >  NB501016:282:H2V7GAFX2:4:21406:8286:19352/1‑147 (MQ=21)
                                           ACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCT                                                                                                      >  NB501016:282:H2V7GAFX2:1:11312:12484:16028/1‑151 (MQ=22)
                                           ACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATC                                                                                                       <  NB501016:282:H2V7GAFX2:2:11107:2030:14226/150‑1 (MQ=22)
                                            CCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTC                                                                                                     >  NB501016:282:H2V7GAFX2:1:21108:12889:5315/1‑151 (MQ=21)
                                            CCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTC                                                                                                     >  NB501016:282:H2V7GAFX2:3:21608:10867:1728/1‑151 (MQ=21)
                                            CCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCT                                                                                                      >  NB501016:282:H2V7GAFX2:1:11310:26459:9712/1‑150 (MQ=22)
                                              TACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCC                                                                                                   >  NB501016:282:H2V7GAFX2:1:21206:18855:10095/1‑151 (MQ=21)
                                              AACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCC                                                                                                   <  NB501016:282:H2V7GAFX2:3:11402:3078:1739/151‑1 (MQ=22)
                                                CGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGAGGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAG                                                                                                 <  NB501016:282:H2V7GAFX2:3:11405:8630:7691/151‑1 (MQ=20)
                                                CGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAG                                                                                                 <  NB501016:282:H2V7GAFX2:2:21301:21552:4535/151‑1 (MQ=22)
                                                  AGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGG                                                                                               <  NB501016:282:H2V7GAFX2:3:11501:2016:2738/151‑1 (MQ=21)
                                                   GACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGT                                                                                              <  NB501016:282:H2V7GAFX2:1:21105:23331:15295/151‑1 (MQ=21)
                                                     CGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTA                                                                                             >  NB501016:282:H2V7GAFX2:4:21409:13475:11613/1‑150 (MQ=21)
                                                       GCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATT                                                                                          >  NB501016:282:H2V7GAFX2:4:11603:3905:17347/1‑151 (MQ=22)
                                                        CGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTC                                                                                         >  NB501016:282:H2V7GAFX2:4:11608:9771:7307/1‑151 (MQ=22)
                                                         GACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGCGTAATTCG                                                                                        <  NB501016:282:H2V7GAFX2:4:11412:10588:12422/151‑1 (MQ=21)
                                                             CCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCG                                                                                    >  NB501016:282:H2V7GAFX2:4:21612:21723:2237/1‑151 (MQ=21)
                                                             CCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCG                                                                                    <  NB501016:282:H2V7GAFX2:1:21201:24385:11524/151‑1 (MQ=21)
                                                             CCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCACGGTAATTCGCGCC                                                                                    >  NB501016:282:H2V7GAFX2:3:11405:26272:4538/1‑151 (MQ=21)
                                                              CTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCG                                                                                    >  NB501016:282:H2V7GAFX2:1:21112:2875:6220/1‑150 (MQ=21)
                                                                ACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGAC                                                                                  <  NB501016:282:H2V7GAFX2:1:11305:14187:17971/150‑1 (MQ=22)
                                                                   GGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTC                                                                              >  NB501016:282:H2V7GAFX2:3:21509:15322:13658/1‑151 (MQ=21)
                                                                    GCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCC                                                                             <  NB501016:282:H2V7GAFX2:2:11302:11117:5109/151‑1 (MQ=21)
                                                                    GCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCC                                                                             <  NB501016:282:H2V7GAFX2:2:11210:19929:13769/151‑1 (MQ=22)
                                                                      GGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTG                                                                           <  NB501016:282:H2V7GAFX2:3:21610:5074:1044/151‑1 (MQ=21)
                                                                      GGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTG                                                                           <  NB501016:282:H2V7GAFX2:2:11301:12785:2743/151‑1 (MQ=22)
                                                                         ATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTT                                                                        >  NB501016:282:H2V7GAFX2:2:21309:13730:6745/1‑151 (MQ=22)
                                                                          TAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTT                                                                        >  NB501016:282:H2V7GAFX2:3:11601:20884:8161/1‑150 (MQ=22)
                                                                          TAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTT                                                                        >  NB501016:282:H2V7GAFX2:1:21111:20438:12228/1‑150 (MQ=22)
                                                                            ACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATG                                                                     >  NB501016:282:H2V7GAFX2:2:21302:13632:1808/1‑151 (MQ=21)
                                                                             CCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGC                                                                    >  NB501016:282:H2V7GAFX2:2:11104:23033:3144/1‑151 (MQ=21)
                                                                             CCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGC                                                                    <  NB501016:282:H2V7GAFX2:4:11610:5798:17137/151‑1 (MQ=22)
                                                                             CCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGC                                                                    >  NB501016:282:H2V7GAFX2:4:11507:5775:6721/1‑151 (MQ=22)
                                                                                   CGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCG                                                              >  NB501016:282:H2V7GAFX2:1:11305:1737:4283/1‑151 (MQ=21)
                                                                                   CGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGG                                                                                               <  NB501016:282:H2V7GAFX2:1:21103:2081:19415/118‑1 (MQ=23)
                                                                                    GGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGG                                                             >  NB501016:282:H2V7GAFX2:2:11208:22915:15970/1‑151 (MQ=21)
                                                                                              GGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTC                                                    >  NB501016:282:H2V7GAFX2:2:21307:13714:13351/1‑150 (MQ=22)
                                                                                                CGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCAC                                                  >  NB501016:282:H2V7GAFX2:3:11601:1159:12066/1‑150 (MQ=21)
                                                                                                 GCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAG                                                <  NB501016:282:H2V7GAFX2:3:11408:2039:9651/151‑1 (MQ=21)
                                                                                                 GCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAG                                                <  NB501016:282:H2V7GAFX2:2:21105:16100:1208/151‑1 (MQ=22)
                                                                                                       AACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCC                                          >  NB501016:282:H2V7GAFX2:2:11310:4740:13980/1‑151 (MQ=21)
                                                                                                        ACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCG                                         <  NB501016:282:H2V7GAFX2:4:21601:12315:7968/151‑1 (MQ=21)
                                                                                                        ACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCG                                         >  NB501016:282:H2V7GAFX2:2:11204:23703:6477/1‑151 (MQ=21)
                                                                                                        ACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCG                                         >  NB501016:282:H2V7GAFX2:4:21610:25797:11010/1‑151 (MQ=22)
                                                                                                         CCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGT                                        >  NB501016:282:H2V7GAFX2:2:11308:7103:9158/1‑151 (MQ=22)
                                                                                                              ACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAG                                   <  NB501016:282:H2V7GAFX2:2:11312:8352:8011/151‑1 (MQ=22)
                                                                                                              ACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCA                                     >  NB501016:282:H2V7GAFX2:3:11407:24077:11508/1‑149 (MQ=22)
                                                                                                                   CTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTG                              >  NB501016:282:H2V7GAFX2:4:21408:5932:16976/1‑151 (MQ=21)
                                                                                                                     ACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGG                            >  NB501016:282:H2V7GAFX2:1:21110:9662:13924/1‑151 (MQ=21)
                                                                                                                     ACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTG                                       >  NB501016:282:H2V7GAFX2:3:21501:1612:19307/1‑140 (MQ=22)
                                                                                                                       GGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCT                          >  NB501016:282:H2V7GAFX2:2:21308:20440:19829/1‑151 (MQ=22)
                                                                                                                           TCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTG                       <  NB501016:282:H2V7GAFX2:4:11403:18780:3627/150‑1 (MQ=21)
                                                                                                                            CGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGG                      <  NB501016:282:H2V7GAFX2:2:11311:16628:6376/150‑1 (MQ=21)
                                                                                                                                 GACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTT                >  NB501016:282:H2V7GAFX2:3:21512:10093:17712/1‑151 (MQ=21)
                                                                                                                                  ACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCG                    <  NB501016:282:H2V7GAFX2:1:11304:21486:3739/146‑1 (MQ=21)
                                                                                                                                   CTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGT              >  NB501016:282:H2V7GAFX2:3:11405:7236:10295/1‑151 (MQ=21)
                                                                                                                                   CTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGT              >  NB501016:282:H2V7GAFX2:4:21401:23223:6197/1‑151 (MQ=21)
                                                                                                                                   CTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGT              >  NB501016:282:H2V7GAFX2:3:11601:20301:18321/1‑151 (MQ=22)
                                                                                                                                     TCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTG             <  NB501016:282:H2V7GAFX2:1:21209:1769:16657/150‑1 (MQ=22)
                                                                                                                                     TCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTG             <  NB501016:282:H2V7GAFX2:1:21306:7401:11631/150‑1 (MQ=22)
                                                                                                                                      CTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCC           >  NB501016:282:H2V7GAFX2:4:21506:17511:16438/1‑151 (MQ=22)
                                                                                                                                      CTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAG                                   >  NB501016:282:H2V7GAFX2:2:11309:22655:4037/1‑127 (MQ=23)
                                                                                                                                       TGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCC           >  NB501016:282:H2V7GAFX2:1:11303:24846:3196/1‑150 (MQ=22)
                                                                                                                                        GCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAC         >  NB501016:282:H2V7GAFX2:4:21603:22577:1354/1‑151 (MQ=21)
                                                                                                                                        GCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAC         >  NB501016:282:H2V7GAFX2:1:21109:3789:3298/1‑151 (MQ=21)
                                                                                                                                        GCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCC           <  NB501016:282:H2V7GAFX2:2:21103:14549:15775/149‑1 (MQ=21)
                                                                                                                                         CACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACC        <  NB501016:282:H2V7GAFX2:4:11406:13467:14508/151‑1 (MQ=22)
                                                                                                                                           CGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTC      >  NB501016:282:H2V7GAFX2:4:21411:11043:19085/1‑151 (MQ=21)
                                                                                                                                               CATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NB501016:282:H2V7GAFX2:3:21605:21419:1202/1‑151 (MQ=22)
                                                                                                                                                |                                                                                                                                                     
CGGGATGACAGTTTCCGCTGTGGGAAATTTCTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACCTCACCC  >  NC_002947_CJ‑RC/4348668‑4348961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑