Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F11 I4 R1	73	908.3	39027734	99.3%	38754539	136.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	1,499,480	2 bp→AC	intergenic (+57/‑106)	trpS CDS (Tryptophan‑‑tRNA ligase) → / → zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	1,499,480	0	C	A	91.9%	590.9 / ‑0.4	286	intergenic (+57/‑107)	trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain)	–/–
Reads supporting (aligned to +/- strand): ref base C (17/6); new base A (131/131); total (148/138)
Fisher's exact test for biased strand distribution p-value = 3.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	AE015451_phaM1	1,499,481	0	A	C	95.5%	701.4 / ‑3.0	286	intergenic (+58/‑106)	trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain)	–/–
Reads supporting (aligned to +/- strand): ref base A (7/6); new base C (141/132); total (148/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

CGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTG‑CCGG‑TG‑T‑T‑T‑CGGG‑CA‑C‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATC  >  AE015451_phaM1/1499347‑1499608

||

  cAGTTCGACAACGCTGAAGCACGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCATGCCCGCGGAAAAGGCGCCGTGGTGTATGGCAAC‑GGc                                                                                                                                       >  2:11059863/1‑137 (MQ=255)

    gTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCtt                                                                                                                                     <  1:16075181/137‑1 (MQ=255)

    gTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCtt                                                                                                                                     >  2:15866356/1‑137 (MQ=255)

    gTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCtt                                                                                                                                     <  1:13893984/137‑1 (MQ=255)

    gTTCGACAACGCTGAAGCCCGTGATGCGACCATTCAGATCCTGAACGAAGCCGTGGCACCACAGCAGGACGGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCtt                                                                                                                                     <  2:8855977/137‑1 (MQ=38)

      tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTg                                                                                                                                   <  1:4708741/137‑1 (MQ=255)

      tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTg                                                                                                                                   <  1:8371441/137‑1 (MQ=255)

      tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTg                                                                                                                                   <  1:8767752/137‑1 (MQ=255)

      tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTg                                                                                                                                   <  1:1542127/137‑1 (MQ=255)

      tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTg                                                                                                                                   <  2:18362769/137‑1 (MQ=255)

      tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTg                                                                                                                                   <  2:3848216/137‑1 (MQ=255)

      tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTg                                                                                                                                   <  2:4222761/137‑1 (MQ=255)

      tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTg                                                                                                                                   <  1:10382599/137‑1 (MQ=255)

            aCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGg‑tg                                                                                                                           <  1:18042769/137‑1 (MQ=255)

                tGAAGCCCGTGATGCGGCCATTCAGAGCCTGCACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑t‑tcg                                                                                                                     >  2:13896775/1‑135 (MQ=255)

                tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑t‑tcg                                                                                                                     >  2:6507713/1‑135 (MQ=255)

                          gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAAGAGCCGCGGTGCATGGCACC‑GGTTTTT‑CCGG‑TG‑T‑T‑TACGGG‑CA‑c‑g‑cc                                                                                                      >  1:6044929/1‑137 (MQ=255)

                          gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCGCGAAACAGGAGCCGGGGGGCACGGCACC‑CGCTTTT‑TCGG‑TG‑T‑T‑TGCGGG‑GA‑c‑g‑cc                                                                                                      >  1:128771/1‑137 (MQ=255)

                          gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑c‑a‑cc                                                                                                      >  2:19005410/1‑137 (MQ=25)

                          gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCACGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑c‑a‑cc                                                                                                      >  2:13168356/1‑137 (MQ=25)

                          gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACAAAAAAAGCGCCCCGGTGCATGGCCCC‑AGCTTTT‑TCAG‑TT‑T‑TCG‑CGGG‑CA‑c‑g‑cc                                                                                                      >  1:7585657/1‑137 (MQ=25)

                                 ctaTTCAGAGCTTGAACGAAGCCTGGGCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGTGGGTGTGCCCGCGAAACGGGCGCTGCTGGGCCTGGCACT‑GGTTTTG‑CTGG‑TG‑T‑TAG‑CGGG‑CA‑C‑GCCCGCTccc                                                                                                <  2:12141575/135‑1 (MQ=255)

                                 ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCAGTGGCTGGCACC‑GGCTTTG‑CCTG‑TT‑T‑TCT‑CGGG‑CA‑C‑GCCCGCTccc                                                                                                >  2:15223691/1‑137 (MQ=25)

                                 ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAAAAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTccc                                                                                                >  2:13985222/1‑137 (MQ=21)

                                   aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCAG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCcaa                                                                                              >  2:14395035/1‑136 (MQ=21)

                                   aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGAGGTGCATGGCACC‑GGCTTTG‑CCAG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCcac                                                                                              >  2:5956804/1‑137 (MQ=21)

                                             tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑A‑GCCCGCTCCCACAGACTGATTg                                                                                    >  2:16317676/1‑137 (MQ=18)

                                              gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGTGAAACAGGAGCAGCGGTGTATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑T‑TGCGGG‑CA‑C‑GCCCGCTCCCACGGGATGATTGc                                                                                   >  2:3079256/1‑137 (MQ=21)

                                              gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCTCTCCCACAGACTGATTGc                                                                                   >  2:12420983/1‑137 (MQ=21)

                                              gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGc                                                                                   >  2:9157170/1‑137 (MQ=21)

                                              gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCACGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGAATGATTGc                                                                                   >  2:14989420/1‑137 (MQ=21)

                                              gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAAAAAGCGCCACGGTGCACGGCCCC‑TGATCTG‑CCGG‑TT‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCACCCAGAATGATTGc                                                                                   >  1:14574739/1‑137 (MQ=19)

                                                 cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAAGCGCCACGGTGCCTTGCCCC‑AGCTTTG‑CCGG‑TT‑T‑TCG‑CTGG‑CC‑CAC‑CCGCTCCAACAGAATGATTGccct                                                                                >  2:1661529/1‑135 (MQ=255)

                                                 cGAAGCCTTGGCACCAAAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACAAGCGCCTCGGTGCATGGCAAC‑GGTTTTG‑CCAG‑TA‑T‑TCG‑CGGG‑CA‑CAA‑CCGCTCCCACAGACCGGTTTCCAt                                                                                >  2:12339615/1‑137 (MQ=18)

                                                  ggcaccTTGTCACCACAATAATACTGAGTTGACCAAATGTTGGAGGCGGGAGTGCCCGCGAAAAAGGCGCCGTGGTGCAGGGCCCT‑GGTTGTT‑TAGG‑TG‑T‑T‑C‑GGGGACA‑A‑GCCCGCTCCCACAGACTGATTGCCAtt                                                                               <  2:5112977/133‑1 (MQ=255)

                                                  gcaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTAGGAGCGGGCGTGCCCGCGAAAAAAGCGCCGCCGTGCATGGCAAC‑GGCCTTG‑CCGG‑TG‑T‑TCT‑CGGG‑CA‑C‑GCCCCCTCCCACAGACTGATCGCCAtt                                                                               >  2:13459259/3‑137 (MQ=21)

                                                  gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAAAAAGCGCCACGGTGCATGGCACC‑AGCTTTT‑CCGG‑TGTT‑T‑T‑TGGG‑CA‑CAC‑CCGCTCCCCCAGACTGACTTCCAtt                                                                               >  1:14189225/1‑137 (MQ=255)

                                                  gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGTATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCAtt                                                                               >  2:17841217/1‑137 (MQ=21)

                                                  gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGAGCCACAGTGTCTGGCCCC‑AGGTTTT‑CCGG‑TG‑T‑TCG‑CGGG‑AA‑C‑GCCCGCTCCCACAGACTGATTGTCAtt                                                                               >  1:13518501/1‑137 (MQ=18)

                                                  gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCCTGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGAATGATTGCCAtt                                                                               >  2:17929630/1‑137 (MQ=21)

                                                  gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCATGGCACC‑AGCTTTG‑CCGG‑TG‑T‑T‑TGCGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCAtt                                                                               >  2:10497540/1‑137 (MQ=25)

                                                  gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAAGGTGGGAGCGGGCGTGTCCGCGAAACAGACGCTGAGATGTCTGTCACC‑GGCTTTG‑CCGG‑TG‑T‑TCA‑CGGG‑TA‑C‑GCCCGCTCCCAAAGACCGATTGCCAtt                                                                               >  2:10588537/1‑137 (MQ=19)

                                                  gAAGCCTTGCCACCACAACAGGACTGAGGCTGCAAACGGGGGGGAGCGGGCGTGCCCGCGAAACGGGCGCCGCGGGGCAGGGCACC‑GGCTTTG‑CAGG‑TG‑TCC‑G‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCAtt                                                                               <  2:9231687/137‑1 (MQ=12)

                                                      ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAGGCGCCGCGGTGCATGGCAAC‑GGATTTG‑CCAG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACCGATTGCCATTCAGc                                                                           >  2:17165786/1‑137 (MQ=21)

                                                      ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGAGAAACAGGCGCCGCGGTGTATGGCACC‑GGCTTTG‑CCAG‑TG‑T‑TCG‑CGGG‑CC‑C‑GCCCGCTCCCACAGGCTGATTGTCATTAAGc                                                                           >  2:10096653/1‑137 (MQ=18)

                                                      ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAAAAAGCGCCACGGTGCACGGCACC‑GGATTTT‑TCGG‑TG‑T‑TCT‑CGGG‑CA‑C‑GCCCACTCCCACAAACTGATTGCCCTTAAga                                                                           >  1:9116470/1‑136 (MQ=255)

                                                       cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGACCACGAAACAAGCGCCGCGGTGCCTGGTACC‑GGCTTTT‑TCGGTTT‑T‑T‑T‑TGGG‑TA‑C‑GCCCCCCCCCACAAAATAATTGCCCTTCAGCg                                                                          >  1:3447035/1‑137 (MQ=255)

                                                        ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCAAC‑GGATTTG‑CCGG‑TG‑T‑TCG‑CGGG‑AA‑C‑GCCCACTCCCACAGAATGATTGCCCTTAAGCgg                                                                         >  2:2554355/1‑137 (MQ=21)

                                                        ttGGCACCACAGAAGGATTGGGGTGGCAAAATGGTGGGAGTGGGTGTTCCCGAGAAACAGGCGCTG‑GGGGGAGGGGCACGGGTTTTG‑CTGG‑TG‑T‑T‑TGGGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                         <  1:1105792/137‑1 (MQ=255)

                                                          ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCATGGCACC‑AGCCTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCCCCCACAAACTGAGTGCCAATAAGCgggg                                                                       >  2:785359/1‑136 (MQ=21)

                                                          ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGTCCGCGAAAAAGGCGCCGCGGTGCATGGCACC‑GGGTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTTCCACAGACTGATTGCCATTCAGCgggg                                                                       >  2:7964594/1‑136 (MQ=21)

                                                           gaaccacGGAAGGATTGGGGTTGCCAAACGGTGGGAGTGGGCGGGCCCGCGAAAAGGGCGCTGTGGTGAGTGGCACT‑GGCTTTG‑CTGG‑TG‑T‑TCG‑GGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                      <  1:12141205/135‑1 (MQ=12)

                                                           gCACCGCAGCGGGGCGGGGGTTGCCATCTGGGGGGAGTGGTTGTGCCTGCGAAAAAGGCGCTTTGGGGCGTGGCACC‑GGTTTTG‑CTGG‑TG‑T‑TCT‑CGGG‑CA‑C‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                      <  1:19251508/137‑1 (MQ=255)

                                                           gCACCAAAGCAGGACTGAGGTTGCCAAACGGTGGGAGCGGGCGTGCCTGCGAAAAGGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑T‑CGCGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                      <  2:4859200/137‑1 (MQ=18)

                                                            caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAGGAGACACGGTGCATGGCACC‑AGCTTTG‑CCGG‑TG‑T‑T‑TGCGGG‑TA‑CAC‑CCTCTCCCCCAGACTGACTGCCCTTCCGCGGGCCt                                                                     >  1:7326042/1‑137 (MQ=255)

                                                            caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAGGCGCCGCGGTGCCTGGCACC‑GGCTTTG‑CCGG‑TG‑T‑T‑CGCGGG‑CA‑C‑GCCCGATCCCACAGACTGATTGCCCTTAAGCGGGctt                                                                     >  2:8626533/1‑135 (MQ=18)

                                                             accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAAGAGCCGCGGTGCATGGCACC‑AGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑A‑GCCCACTCCCACAGAACGATTGCCACTCAGCGGGCCTa                                                                    >  1:18645930/1‑137 (MQ=14)

                                                                acaGCAGGACTGAGGCTACTAAATGGTGGGAGCGGGCGTTCCCTCGAAACAGGCCCCGCGGTACGTGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑TGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg                                                                 <  2:4428047/137‑1 (MQ=18)

                                                                   gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa                                                              >  2:9120134/1‑137 (MQ=21)

                                                                   gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAAGCGCCGCGGTGTACGGCACC‑AGCTTTT‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑CAC‑CCGCTCCCCCAAACCGACTTCCACTCAGCGGGCCTATCGCtc                                                              >  2:1613244/1‑136 (MQ=255)

                                                                         cTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCGCGAAACAGGCGCCACGGTGCATGGCACC‑GGCTTTG‑CCAG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGACTGCCACTCAGCGGGACTATCACTACAGTGa                                                        >  2:16831169/1‑137 (MQ=17)

                                                                          tGAGTCTGCCAAACGTGGGAAGCGGGTTTGCCCGCGAAACGGGCGCCGCGGTGCGTGGCACC‑GGCTTTT‑CCGT‑TG‑T‑TCG‑CGGG‑CA‑C‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc                                                       <  2:3502440/137‑1 (MQ=255)

                                                                            aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGTACGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCTCTCCCCCAAAACTACTTCCATTAAAAAGGCCTATCACCACAGTGACgg                                                     >  2:6797797/1‑137 (MQ=9)

                                                                            aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCACCGCGGTGCATGGCACC‑GGATTTG‑CCGG‑TG‑T‑TCG‑CTGG‑CA‑C‑GCCCGCTCCCCCCAAATGACTTACATTAAGTGGGCCTACCGCTACAGTGACgg                                                     >  2:17111026/1‑137 (MQ=9)

                                                                               ctacaaaaCGGGGGGGGGGGGGGTGCCCGAGAAACAGGCGCTGCGGGGAATGGCACT‑GTTTTTT‑CCGG‑TG‑T‑TCG‑TGGG‑CA‑C‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                  <  1:17479890/132‑1 (MQ=255)

                                                                               cTGCCAAACGGTGGGAGCGGGTGTGTCCTTGAAACAGGTGTCTCGGTGTCTGGTACC‑GGCTTTT‑TCGG‑TG‑T‑T‑TGCGGG‑TA‑C‑GCCCGCTCCCCCAGACCGGTTGCCATTCAGCGGGTCTCTCTCTACAGTGACGGccc                                                  >  2:15960531/1‑137 (MQ=255)

                                                                                 gCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACC‑GGCTTTG‑CCGG‑TG‑T‑T‑CGCGGG‑CA‑C‑GACCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTCCAGTGACGGCCCGt                                                >  2:18547803/1‑137 (MQ=21)

                                                                                    aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCAGTGCATGGCCCC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGACTGCCATTAAGAAGGACTATTGCTAAAGTGACGGTCCGtttt                                             >  2:9729314/1‑137 (MQ=17)

                                                                                     aaCGGTGGGAGCGGGCGTGTCCGCGGAACAGGCGCCGCGGTGCATGGCACC‑GGATTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGAGGGCCTATCGCTACAATGACGGCCCGctttt                                            >  2:11547479/1‑137 (MQ=18)

                                                                                         gTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑T‑CGCGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTTCCATTAAGCGGGCCTCTCGCTACAATGACGGCCCGGTTTttgtt                                        >  2:16147671/1‑137 (MQ=18)

                                                                                          tGGGAGCGGGTGTGCCCGAGAAAAGGGCGCCGCGGGGCGTGACACT‑GGCTTTT‑CCGG‑TT‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg                                       <  2:16683899/137‑1 (MQ=21)

                                                                                          tGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGTGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg                                       <  2:19275366/137‑1 (MQ=21)

                                                                                            ggAGTGGGCGTTCCTGTAAAACAGGCTCTGCGGTGCAGGACCCT‑GTTTTTT‑TTGG‑TG‑T‑T‑T‑GGGGACACC‑C‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGcc                                     <  1:3802861/137‑1 (MQ=255)

                                                                                               gCGGGTGTGTCCGCGAAATAGGCGCCGCGGTGCATGGAACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGAATGATTGCCATTAAGCGGGACTATCGCTACAGTGACGGCCCGTTTTTTGTTGCATCg                                  >  2:19213887/1‑137 (MQ=21)

                                                                                                tgtttttGCCCGTGAAACAGGCCCCGTGGGGCGCGACACC‑GGCTTTG‑CCGG‑TG‑T‑T‑CGAGGA‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAGCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc                                 <  2:10692027/131‑1 (MQ=19)

                                                                                                ggggTGTGCCGGCGAAAAGGGCGCCGTGGTACAGGGCACT‑GGTTTTG‑CCGG‑TG‑T‑T‑C‑GGGGACA‑C‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc                                 <  1:13431228/136‑1 (MQ=255)

                                                                                                   gtgTGCCTTCGAAACGTGCGCCGTGGTGCGTGGCACT‑GTCTTTG‑CTGG‑TG‑T‑T‑CGTGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa                              <  1:2380521/135‑1 (MQ=19)

                                                                                                        cccGCGAAACGTGCGCCGCGGTGCATGGCACC‑GTTTTTG‑CTGG‑TG‑T‑TCG‑TGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGTCTCGCTAACGAAt                         <  2:9419672/137‑1 (MQ=25)

                                                                                                        cccGAGAAAAGGCCGCCGGGGGGCATGGAACC‑GGTTGTG‑CCGG‑TG‑T‑TAG‑GGGG‑CA‑C‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt                         <  1:17764803/137‑1 (MQ=255)

                                                                                                          cgtgAAACAGGCGCCATGGTGCATGGCACC‑GGTTTTT‑CTGG‑TG‑T‑TCG‑TGGG‑CA‑T‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:8974696/134‑1 (MQ=255)

                                                                                                          cgcgAAACGGTCGCCGTGGTGCATGGCACC‑GTCTTTG‑CCGT‑TT‑T‑TCG‑CGGA‑CA‑C‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACNGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  1:18811845/137‑1 (MQ=255)

                                                                                                          cgcgAAACAGGTGCCGAGGTGAGTGTCACA‑GGCTTTG‑CCGG‑TG‑T‑TCG‑TGGA‑CA‑C‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:7664033/137‑1 (MQ=255)

                                                                                                          cgcgAAACAGGCGCCGCGGTGCATGGCACT‑GGTTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:14559781/137‑1 (MQ=25)

                                                                                                          cgcgAAAAAGGCGCCGCGGTACATGGCACC‑GGCTTTT‑CTGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  1:18364348/137‑1 (MQ=25)

                                                                                                          cgcaaaaCAGACCCCGCGTGGAATGGCACC‑GGTTTTG‑CCGG‑TG‑T‑TCG‑TGCG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:702107/133‑1 (MQ=21)

                                                                                                          cgcaAAACAGGCGCCACGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑TGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  1:13625716/137‑1 (MQ=25)

                                                                                                          cgcaAAAAAGGCGCCGCGGTGAATGACACC‑GGTTTTG‑CTGG‑TT‑T‑T‑TGAGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:6432181/137‑1 (MQ=255)

                                                                                                          cgagAAAAAGGCGTCGTGGTGCAGGGCACT‑GGTTTTG‑CTGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  1:16714405/134‑1 (MQ=25)

                                                                                                           gcgAAACGGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                      <  2:13636591/137‑1 (MQ=25)

                                                                                                           gcgAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑ACCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                      <  2:18740373/137‑1 (MQ=25)

                                                                                                           gcgAAACAGGCGCCGCGGTGCAGGGCACC‑GGCTTTT‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                      <  2:11731394/137‑1 (MQ=37)

                                                                                                           gcgAAACAGGCGCCGCGGGGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑T‑CGCGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                      <  2:19466132/137‑1 (MQ=37)

                                                                                                           gcaaaaCAGGCCCCGCGGTACAGGGCACC‑GGCTTTC‑CCGG‑TT‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                      <  2:5654137/134‑1 (MQ=25)

                                                                                                               aaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       >  1:540212/1‑132 (MQ=37)

                                                                                                               aaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑T‑CGCGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:540212/132‑1 (MQ=25)

                                                                                                                     cgccgtGGTACGTGGCACC‑GGCTTTT‑CCGG‑TT‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa            <  2:13136217/137‑1 (MQ=25)

                                                                                                                     ccccctggTGCATGG‑CAC‑GGGTTTGACCGG‑TG‑TCC‑G‑CGGG‑CA‑C‑ACCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa            <  1:7484363/131‑1 (MQ=255)

                                                                                                                       ccgcGGTGCATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGa          >  1:10129997/1‑137 (MQ=37)

                                                                                                                               cATGGCACC‑GGTTTTG‑CCGG‑TG‑T‑TCG‑CGGA‑CA‑C‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc  <  1:6737480/137‑1 (MQ=255)

                                                                                                                               cATGGCACC‑GGCTTTG‑CCGG‑TG‑T‑TCG‑CGGG‑CA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc  <  2:12693782/137‑1 (MQ=37)

||

CGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTG‑CCGG‑TG‑T‑T‑T‑CGGG‑CA‑C‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATC  >  AE015451_phaM1/1499347‑1499608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑TT‑‑T‑‑CGGG‑‑CA‑CG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACG  >  AE015451_phaM1/1499333‑1499624

GGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGTCCGCGAAACAAGAGCCGCGGTGCCTGGCCCCG                                                                                                                                                         >  LH00941:114:22FVMWLT1:2:2266:9052:1945/1‑151 (MQ=60)

  TTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGC                                                                                                                                                       <  LH00941:114:22FVMWLT1:2:1126:46809:15556/151‑1 (MQ=60)

  TTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGC                                                                                                                                                       <  LH00941:114:22FVMWLT1:2:1126:46800:15540/151‑1 (MQ=60)

   gtgtataagagacagGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCANCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCT                                                                                                                                                      <  LH00941:114:22FVMWLT1:2:2249:45043:6701/136‑1 (MQ=60)

   TGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAGCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCT                                                                                                                                                      <  LH00941:114:22FVMWLT1:2:1123:45228:28574/151‑1 (MQ=60)

   TGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCT                                                                                                                                                      <  LH00941:114:22FVMWLT1:2:1233:11225:19062/151‑1 (MQ=60)

   TGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCT                                                                                                                                                      <  LH00941:114:22FVMWLT1:2:1102:48658:22185/151‑1 (MQ=60)

        ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC                                                                                                                                                 <  LH00941:114:22FVMWLT1:2:2146:8202:23050/151‑1 (MQ=60)

        ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC                                                                                                                                                 <  LH00941:114:22FVMWLT1:2:1127:21783:21977/151‑1 (MQ=60)

          GGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGG                                                                                                                                               <  LH00941:114:22FVMWLT1:2:1141:40818:14851/151‑1 (MQ=60)

          GGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGG                                                                                                                                               <  LH00941:114:22FVMWLT1:2:1214:34356:28158/151‑1 (MQ=60)

             CCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑C                                                                                                                                           <  LH00941:114:22FVMWLT1:2:2201:5641:17301/151‑1 (MQ=60)

             CCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑C                                                                                                                                           <  LH00941:114:22FVMWLT1:2:2201:5650:17317/151‑1 (MQ=60)

                CAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCTCGAAACAGGCCCCACGGGGCATGGCCCCAGCTTTGTCGGTG‑TTct                                                                                                                                        >  LH00941:114:22FVMWLT1:2:2221:27321:25452/1‑149 (MQ=60)

                  GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCGCGAAACAAGAGCCGCGGTGCATGGCCCCAGCTTTGCCGGTtttcgag                                                                                                                                       >  LH00941:114:22FVMWLT1:2:2149:25037:14483/1‑144 (MQ=60)

                  GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGccatgc                                                                                                                                       <  LH00941:114:22FVMWLT1:2:2160:39727:25580/151‑7 (MQ=60)

                  GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGccatgc                                                                                                                                       <  LH00941:114:22FVMWLT1:2:1219:17022:1304/151‑7 (MQ=60)

                  GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGccatgc                                                                                                                                       <  LH00941:114:22FVMWLT1:2:2222:37056:8622/151‑7 (MQ=60)

                  GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACGGAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGccatgc                                                                                                                                       <  LH00941:114:22FVMWLT1:2:2131:21653:22361/151‑7 (MQ=60)

                    TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGccatgcac                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1168:18445:23690/151‑9 (MQ=60)

                    TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGccatgcac                                                                                                                                     <  LH00941:114:22FVMWLT1:2:2155:1296:15123/151‑9 (MQ=60)

                    TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGccatgcac                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1262:13102:3162/151‑9 (MQ=60)

                    TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcggg                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1123:26156:13922/151‑7 (MQ=60)

                    TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcggg                                                                                                                                    <  LH00941:114:22FVMWLT1:2:2109:13832:29631/151‑7 (MQ=60)

                    TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcggg                                                                                                                                    <  LH00941:114:22FVMWLT1:2:2109:13814:29631/151‑7 (MQ=60)

                    TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcggg                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1256:28143:22105/151‑7 (MQ=60)

                    TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcggg                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1256:28134:22121/151‑7 (MQ=60)

                    TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcggg                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1251:23160:4923/151‑7 (MQ=60)

                    TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcggg                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1246:37869:15347/151‑7 (MQ=60)

                    TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCTTTGCCGGTGccatgcac                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1168:18464:23690/151‑9 (MQ=60)

                    TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTGTGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcggg                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1256:28153:22121/151‑7 (MQ=60)

                          ACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAAGAGCCGCGGTGCATGGCACCGGTTTTTCCGGTG‑TT‑‑TA‑CGGG‑‑CA‑CG‑C‑C                                                                                                                      >  LH00941:114:22FVMWLT1:2:1218:3431:4731/1‑151 (MQ=60)

                          ACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCtgtctcttatacacatctccgagcccacg                                                                                                                               >  LH00941:114:22FVMWLT1:2:1226:47049:13922/1‑122 (MQ=60)

                          ACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CG‑C‑C                                                                                                                      <  LH00941:114:22FVMWLT1:2:2154:18362:10255/151‑1 (MQ=60)

                          ACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CG‑C‑C                                                                                                                      <  LH00941:114:22FVMWLT1:2:2250:37499:7437/151‑1 (MQ=60)

                          ACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACAAAAAAAGCGCCCCGGTGCATGGCCccagctttttcagttttcgcgggcacgcc                                                                                                                               >  LH00941:114:22FVMWLT1:2:1240:42806:1865/1‑122 (MQ=60)

                          ACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGGGGCCACGAAACAAGCGCCACGGTGCCTGGCCACGGATTTTTCGGTGTTT‑‑T‑‑TGGG‑‑CA‑CG‑C‑C                                                                                                                      >  LH00941:114:22FVMWLT1:2:1150:21163:23530/1‑151 (MQ=60)

                           CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCACGAAACAAGAGCCACGGTGCATGGCACCAGCTTTGCCGGTG‑TTcgctggcacaccc                                                                                                                             >  LH00941:114:22FVMWLT1:2:2128:42713:22137/1‑138 (MQ=60)

                            GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCtgtctcttatacacatctccgagcccacgag                                                                                                                             >  LH00941:114:22FVMWLT1:2:1127:38553:15251/1‑120 (MQ=60)

                                                 ATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCTAAACAAGCTACGCGGTGCCTGGCACCCGCTTTGCCGGTG‑TT‑‑TG‑CTGG‑‑CA‑CGAC‑CGCTCCCCCAGACTTATTGCCAT                                                                                                >  LH00941:114:22FVMWLT1:2:1215:11678:18278/1‑151 (MQ=60)

                                                  TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCGCGAAAAAGGAGCCGCGGTGTAGGGCACCGGCTGTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CG‑CACGCTCCCACAGAACGATTGCCATT                                                                                               >  LH00941:114:22FVMWLT1:2:2159:34596:5900/1‑151 (MQ=60)

                                                  TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAAGAGCCGCAGTGCCTGGCACCGGCTTTTCCGGTG‑TT‑‑Tttgggcacgcccgctcccccaaaacgagtgccact                                                                                                    >  LH00941:114:22FVMWLT1:2:2148:37971:13858/1‑116 (MQ=60)

                                                  TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAAAAAGCGCCGTGGTGCCCGGCACCTGCTTTGCCGGTG‑TT‑‑TG‑CGGG‑‑CA‑CGCC‑CGCTCCCACcaaccgagtgccatt                                                                                               >  LH00941:114:22FVMWLT1:2:2247:14951:5884/1‑136 (MQ=60)

                                                  TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCGCGAAAAAGGAGCCGCGGTGCATGGTACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGAATGGTTGCCACT                                                                                               >  LH00941:114:22FVMWLT1:2:1255:43185:11328/1‑151 (MQ=60)

                                                  TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCTCGAAAAAGGAGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATT                                                                                               >  LH00941:114:22FVMWLT1:2:2155:3191:14563/1‑151 (MQ=60)

                                                  TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCGGCCTTTCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAAAATGATTGCCATT                                                                                               >  LH00941:114:22FVMWLT1:2:2251:40421:20215/1‑151 (MQ=60)

                                                      GAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAAAAAGCGCCACGGTGCACGGCACCGGATTTTTCGGTG‑TTC‑T‑‑CGGG‑‑CA‑CGCC‑CACTCCCACAAACTGATTGCCCTTAAGA                                                                                           >  LH00941:114:22FVMWLT1:2:1261:16800:19687/1‑151 (MQ=60)

                                                            GAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAGGAGACACGGTGCATGGCACCAGCTTTGCCGGTG‑TT‑‑Tgcgggtacaccctctcccccagactgactgcccttccgcgggcct                                                                                          >  LH00941:114:22FVMWLT1:2:1236:10559:11024/1‑106 (MQ=60)

                                                            GAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCACGAAAAAAGAGCCACGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcgggcaagccccctcccccaaaatgagttccactcaacgggcct                                                                                            >  LH00941:114:22FVMWLT1:2:2127:38313:28254/1‑105 (MQ=40)

                                                             AACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCACGAAACAAGCGCCACGGTGTATGGAAACAGCTTTTCCGGTG‑TTC‑G‑‑CGGGCCCA‑C‑‑C‑CGCTCCCACAGACTGGTTGCCATTCAGTGGGCCTG                                                                                    >  LH00941:114:22FVMWLT1:2:1154:12926:25275/1‑151 (MQ=49)

                                                             AACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAAGAGCCGCGGTGCATGGCACCAGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑AGCC‑CACTCCCACAGAACGATTGCCACTCAGCGGGCCTA                                                                                    >  LH00941:114:22FVMWLT1:2:2258:1361:22569/1‑151 (MQ=41)

                                                                       TGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCACGAAAAAAGCGCCGCGGTGCATGGCCCCGGCCTTTCCGGTG‑TTcgcgggcacacccacccccacaaaccgatttcccttcaacaggcctctcgctaccat                                                                                 >  LH00941:114:22FVMWLT1:2:1266:42473:4779/1‑94 (MQ=60)

                                                                         GCACCACAGCAGGACTGAGGCTACCAAATGGGGGGAGCGGGCGTACCTGCGAAAAAGGCGCCTTGGTGCGTGGCACTGGTTTTTCTGGT‑‑TT‑‑TCGCGGG‑‑CACCC‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA                                                                        <  LH00941:114:22FVMWLT1:2:2157:26840:28942/151‑1 (MQ=35)

                                                                               CAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAAAAGGCGCCACGGTGCATGGCAACAGCTTTGCCTGTG‑TTC‑T‑‑CGGG‑‑CA‑Cacccgctcccccagaacgatttccattacgcgggtccatcgctacaatggaggtaa                                                                   >  LH00941:114:22FVMWLT1:2:1260:17733:14066/1‑95 (MQ=60)

                                                                                     GACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCCAAACAAGCGCCACGGTGCATGGCCccaacctttcctgttttctttggcccccccccccccccaaaactatttcccttcagcaggaacctccccccaacgacagcccactttt                                                                    >  LH00941:114:22FVMWLT1:2:2107:46948:10480/1‑63 (MQ=43)

                                                                                         GAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTTTCGGTGTTT‑‑T‑‑CGGGAACA‑C‑‑C‑CCCTCCCACAGAATGGTTGTCATTCAGCGGGCCTATCGCTACAGTGACGGCCCATTTTTTTTT                                                        >  LH00941:114:22FVMWLT1:2:1147:51737:13106/1‑151 (MQ=55)

                                                                                                       tgtttcGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAATGACGGCCCGTTTTTTGTTGCCTCGCTAACGAA                                          >  LH00941:114:22FVMWLT1:2:1213:47789:3098/7‑151 (MQ=60)

                                                                                                              CGGGCTTGCCCGCGAAACAGGCGCCGCGGGGCATGACACCGGCTTGGCTGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAC                                   <  LH00941:114:22FVMWLT1:2:2169:46042:14803/151‑1 (MQ=60)

                                                                                                               GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCATTTTTTGTTGCCTCGCTAACGAATCATGACT                                  >  LH00941:114:22FVMWLT1:2:1108:46689:27677/1‑151 (MQ=60)

                                                                                                                GGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTC                                 >  LH00941:114:22FVMWLT1:2:1237:40421:22329/1‑151 (MQ=60)

                                                                                                                       CCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGA                          >  LH00941:114:22FVMWLT1:2:2106:43952:8494/1‑151 (MQ=60)

                                                                                                                        CGTGAAACAGGCGCTGTGGTGCATGGCACCGTCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAA                         <  LH00941:114:22FVMWLT1:2:2163:47428:9967/151‑1 (MQ=60)

                                                                                                                        CGCGAAACAGGCGCTACGGTGCATGGCACCGGCTTTGCCGGTG‑TTCGT‑‑‑GGA‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACNGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAA                         <  LH00941:114:22FVMWLT1:2:2260:49721:21208/151‑1 (MQ=60)

                                                                                                                        CGCGAAAAAGGCGCCGCGGTACATGGCACCGGCTTTTCTGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAA                         <  LH00941:114:22FVMWLT1:2:2254:28023:23466/151‑1 (MQ=60)

                                                                                                                        CGCAAAACAGGCGCCACGGTGCATGGCACCGGCTTTGCCGGTG‑TTCGT‑‑‑GGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAA                         <  LH00941:114:22FVMWLT1:2:2157:14100:1368/151‑1 (MQ=60)

                                                                                                                           GAAACAGGCGCCGCGGGGCATGGCACCGGTTTTGCTGGTG‑TTCGT‑‑‑GGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGC                      <  LH00941:114:22FVMWLT1:2:2244:17022:20936/151‑1 (MQ=60)

                                                                                                                                  agagacaGGTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG               <  LH00941:114:22FVMWLT1:2:2262:50655:23754/144‑1 (MQ=60)

                                                                                                                                  GCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG               >  LH00941:114:22FVMWLT1:2:1154:23354:10864/1‑151 (MQ=60)

                                                                                                                                  GCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCACGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG               >  LH00941:114:22FVMWLT1:2:2264:3339:16452/1‑151 (MQ=60)

                                                                                                                                      gcCGGTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGA           >  LH00941:114:22FVMWLT1:2:2113:29188:17125/3‑151 (MQ=60)

                                                                                                                                       GCGGTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAT          >  LH00941:114:22FVMWLT1:2:1240:4365:14643/1‑151 (MQ=60)

                                                                                                                                        CGGTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATC         >  LH00941:114:22FVMWLT1:2:2135:51700:14995/1‑151 (MQ=60)

                                                                                                                                          GTNCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTNNGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:1266:21912:1032/1‑151 (MQ=60)

                                                                                                                                          GTNCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTNNGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:1266:21931:1032/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGCACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:1118:4596:29006/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCTAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2227:9709:21560/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCCAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2163:19749:12593/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2206:35151:12273/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2211:38137:21897/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:1206:51394:29391/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:1122:52180:10191/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2246:33496:6909/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:1142:18945:5948/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2203:17216:3114/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:1104:8627:9823/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2202:1185:10191/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2144:7065:8718/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2106:35854:14323/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2154:27681:10031/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:1256:18316:3546/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:1256:18307:3562/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:1240:10559:27037/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2163:11142:3098/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2163:19758:12577/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2163:19767:12561/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:1238:28393:18854/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:1206:51413:29391/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2206:31111:9855/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCAGTTTTTTGTTGCCTCGCTAACGAATCATGAATCCCTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2101:1240:15027/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACAGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCACTTAGAACGCTATCAAGCAGATCTG       >  LH00941:114:22FVMWLT1:2:2206:35160:12289/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA     >  LH00941:114:22FVMWLT1:2:1141:36880:19431/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA     >  LH00941:114:22FVMWLT1:2:2257:31416:10512/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA     >  LH00941:114:22FVMWLT1:2:1230:51542:7357/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTAAAGTGACGGCCCG‑TTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAA    >  LH00941:114:22FVMWLT1:2:2170:20821:8910/1‑151 (MQ=60)

                                                                                                                                             CATGGCACCGGTTTTGCCGGTG‑TT‑‑TG‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAA    <  LH00941:114:22FVMWLT1:2:1142:21376:19447/151‑1 (MQ=60)

                                                                                                                                               TGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACG  >  LH00941:114:22FVMWLT1:2:2245:4569:17077/1‑151 (MQ=60)

                                                                                                                                               TGGCACCGGCTTTGCCGGTG‑TTC‑G‑‑CGGG‑‑CA‑CGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACG  >  LH00941:114:22FVMWLT1:2:2241:17410:18374/1‑151 (MQ=60)

GGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑TT‑‑T‑‑CGGG‑‑CA‑CG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACG  >  AE015451_phaM1/1499333‑1499624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`