Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F11 I4 R1	74	758.2	33768536	99.3%	33532156	136.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	1,499,480	2 bp→AC	intergenic (+57/‑106)	trpS CDS (Tryptophan‑‑tRNA ligase) → / → zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	1,499,480	0	C	A	92.2%	408.6 / NA	194	intergenic (+57/‑107)	trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain)	–/–
Reads supporting (aligned to +/- strand): ref base C (14/1); new base A (106/71); total (120/74)
Fisher's exact test for biased strand distribution p-value = 1.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	AE015451_phaM1	1,499,481	0	A	C	97.4%	463.3 / ‑6.3	195	intergenic (+58/‑106)	trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain)	–/–
Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (116/71); total (120/75)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑T‑T‑T‑CGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG  >  AE015451_phaM1/1499351‑1499611

||

  tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg                                                                                                                                  <  1:3964874/137‑1 (MQ=255)

  tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg                                                                                                                                  <  2:7854379/137‑1 (MQ=255)

  tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg                                                                                                                                  <  2:16323413/137‑1 (MQ=255)

   cGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGc                                                                                                                                 <  1:15831770/137‑1 (MQ=255)

     acgaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCTTTGGCACCACAGCATGACTGGGGCTGCCAAACGTTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg                                                                                                                               <  2:14902189/134‑1 (MQ=37)

      cAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCGCGAAACAGGCGCCGCGGTGTCTGGCACCAGATTTGCCgg                                                                                                                              >  2:427895/1‑137 (MQ=255)

      cAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAACGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGcctg                                                                                                                              >  2:9720241/1‑135 (MQ=255)

         cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtg‑t                                                                                                                          <  2:668486/137‑1 (MQ=255)

         cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtg‑t                                                                                                                          <  1:9424266/137‑1 (MQ=255)

         cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCAGCTTTGCCGgtg‑t                                                                                                                          >  2:4809709/1‑137 (MQ=255)

            tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCCCGAAACAAGCGCCACAGTTCATTGCCCCAGCTTTTCCCGTG‑t‑t‑tt                                                                                                                     >  1:4971436/1‑136 (MQ=255)

            tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCACGCAAAAGGAGCCTCAGGGCATGGCCCCAGATTTTCCGGTG‑t‑tct                                                                                                                      >  1:13026964/1‑135 (MQ=255)

            tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCACGAAACAAGCGCCACGGTGCCTGGCACCGGCTTTGCCTGTG‑t‑tct                                                                                                                      >  1:11701029/1‑135 (MQ=255)

            tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCACGGCACCGGCTTTGTCGGTG‑t‑tcg                                                                                                                      >  2:6301126/1‑135 (MQ=37)

            tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCATGCCCGCAAAACAGGCGCCGAGGTGCATGGCACCGGCTTTGCCGGTG‑t‑tcg                                                                                                                      >  2:150466/1‑135 (MQ=38)

            tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAAGCGCCACGGTGCACGGCACCGGATTTTTCGGTG‑t‑t‑tt                                                                                                                     >  1:2853386/1‑136 (MQ=255)

              aaGCCCGTGATGTGGCCATTCAGAGCCTGAACGAAGCATTGGCACCACCGCAGGACTGAGGCTGCCAAACGGTAGAAGCGGGCGTGTCCACGGAACAGGCGCCACCGTGCACGGCACCAACTTTGCCGGTG‑t‑tcgcg                                                                                                                    >  2:6108530/1‑133 (MQ=255)

                      gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCGAGAAAAAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAa‑g‑cc                                                                                                         >  2:2441653/1‑137 (MQ=25)

                      gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCGGATTTGCCGGTG‑T‑TCG‑CGGGCAc‑g‑cc                                                                                                         >  2:16218334/1‑137 (MQ=25)

                      gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCCCTGCTTTGTCGGTG‑T‑TCG‑CGGGCAc‑g‑cc                                                                                                         >  2:10817124/1‑137 (MQ=37)

                      gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAGGCGCCACGGGGTATGGCACCCGCTTTTTCCGTG‑T‑TCT‑CGGGCAcaac                                                                                                           >  1:5258733/1‑134 (MQ=255)

                      gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAAGCGCCACAGTGTATGGCACCAGCTTTGCCGGTG‑T‑TCT‑CGGGCAc‑a‑cc                                                                                                         >  2:14170624/1‑137 (MQ=25)

                      gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAAAAAGAGCCGCGGTGCATGGGACCAGATTTTCCGGTG‑T‑TCT‑TGGGCAc‑a‑cc                                                                                                         >  1:10175655/1‑137 (MQ=255)

                       aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCTTTGCCAGTG‑T‑TCG‑CGGGCAc‑g‑ccc                                                                                                        >  2:9600394/1‑136 (MQ=37)

                       aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCGGCTTTGTCGGTG‑T‑TCG‑CGGGCAc‑g‑ccc                                                                                                        >  2:3984472/1‑136 (MQ=25)

                        tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGGCCGCGAAACAAGAGCCACGGTGCCTGGCCCCGGGTTTTCCGGTGTT‑T‑T‑CGGGCAc‑gaccg                                                                                                        >  1:4777954/1‑133 (MQ=255)

                             ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTccc                                                                                                   >  2:6751384/1‑137 (MQ=21)

                             ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGTCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTccc                                                                                                   >  2:3017836/1‑137 (MQ=21)

                             ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTccc                                                                                                   >  2:10071397/1‑137 (MQ=21)

                             ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCATGGCACCGGGTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTccc                                                                                                   >  2:15297598/1‑137 (MQ=18)

                             ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCAAAACAAGCGCCATGGTGCACGGCACCAGCTTTTTCGGTGTT‑T‑T‑CGGGCAC‑GCCCGCTccc                                                                                                   >  1:13872817/1‑137 (MQ=255)

                                    gatccTGAACGAAGCTTTGGCACCACAGCAGGACGGAGGCTGCCAAGCGGTGGGAGCGGGCGTGCCCGTGAAACATGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGACAC‑GCCCGCTCCCACAGACt                                                                                            <  2:15394393/134‑1 (MQ=17)

                                    gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCACGGTGCATGGCACCAGTTTTGCCGGTG‑T‑TCG‑CAGGCACAC‑CCGCTCCCAAAGACt                                                                                            >  2:9820211/1‑137 (MQ=21)

                                    gagCCGGAACGAGGCCTTGGCACCACAGCAGGACTAAGGCTGCCAAACGGTGAGAGCGGGCGTGCCATCGAAACAGGCGCCGCGGTGCATGGCACCGTCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACt                                                                                            <  2:5469515/137‑1 (MQ=18)

                                          gAACGAAGCCTTGGCACCACCGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCACTCCCCCAGAATGATTGc                                                                                      >  2:7079614/1‑137 (MQ=18)

                                          gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGc                                                                                      >  2:10242089/1‑137 (MQ=21)

                                          gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGAATGATTGc                                                                                      >  2:3069670/1‑137 (MQ=21)

                                          gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCAAAGACTGATTGc                                                                                      >  2:12613622/1‑137 (MQ=21)

                                          gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAAAAGGCGCCACGGTGCCTGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAAACTGATTGc                                                                                      >  1:14132766/1‑137 (MQ=18)

                                              gaggtcTTGGCACCACAGAAGGACGGAGGCTGCAAAATGGTGGGATTGGGCGTTCCCGCGAAACAGGTTCCGTGGGGCATGGAACTGGTTTGGTTGGTG‑T‑TCG‑TGGGCAC‑GCCCGCTCCCACAGACTGATTGCCAtt                                                                                  <  2:8813186/132‑1 (MQ=255)

                                              gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGTGGGCGTGCCCGCGGAACAGGCGCCGAGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGATCCCACGGGCTGAGTGCCagt                                                                                  >  2:11254793/1‑135 (MQ=18)

                                              gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCAAAACAGGCGCCGCGGGGCAGGGCACCGGCTTTGTCGGTG‑T‑TCG‑CGGGTAC‑GCCCACCCCCACAAACCAATTGCCact                                                                                  >  1:15265554/1‑135 (MQ=255)

                                              gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCACGAAACAAGCGCCGCGGTGCATGGCACCGGATTTGCCGGTG‑T‑TCG‑CGGGGAA‑GCCCGCTCCCACAGAATGAGTGACAtt                                                                                  >  1:6651840/1‑137 (MQ=255)

                                              gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCCCGAAACAGGAGCCACGGTGTCTGGCCCCAGCTTTGCCGGTG‑T‑T‑TGCGGGCCCAC‑CCACTCCCCCAGACCGGTTGCCact                                                                                  >  1:13507491/1‑135 (MQ=255)

                                              gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGTGCCGCGGTGCATGGCACCAGATTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCACTCCCACAGAATGACTGCCact                                                                                  >  2:5352466/1‑135 (MQ=18)

                                              gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCact                                                                                  >  2:3671859/1‑135 (MQ=21)

                                              gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCAtt                                                                                  >  2:7218445/1‑137 (MQ=18)

                                              gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCACCACGGTGCCTGGCACCCGCCTTTCCGGTG‑T‑TCT‑CGGGCACAC‑CCGCCCCCACAAACCGATTTcccct                                                                                  >  2:5264328/1‑134 (MQ=16)

                                              gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAAGCGCCGCAGTGCATGGCACCAGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCACCCCCACCGAATGATTGCCact                                                                                  >  1:12233814/1‑135 (MQ=17)

                                              gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCAAAATGGTTGAAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCGGGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCAtt                                                                                  <  2:15150973/137‑1 (MQ=18)

                                              gAAGCCTTGGCACCAAAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGGGCCGCGGTGCCTGGCCCCGGCTTTGCCGGTG‑T‑T‑TGCGGGCCCTC‑CCCCTCCCACAGACTGATTGCCact                                                                                  >  2:3046908/1‑135 (MQ=21)

                                                  ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCACGGCACCAGGTTTGCCAGTG‑T‑TCG‑CAGGCAC‑GCCCGCTCCCAAAGAATGATTGCCATTAAGc                                                                              >  2:2832541/1‑137 (MQ=255)

                                                  ccTTGGCACCACAACAGGACTAAGACTGCAAAATGGGGGGAGTGGGTGTGCCCGAAAAACAGGCGCCGTGGTGAGTGGAACTGGTTTTGCTGGTG‑T‑T‑CGTGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGc                                                                              <  1:7558198/137‑1 (MQ=255)

                                                    tttccaccacaccaGAACGGAGCCTACCACCCGTTGGGAGCGGGCGTCCCCGCGAAACGGGCTCCGCGGTGCATGTCACCGTCTTTGCCGGTT‑T‑TCT‑GGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                            <  2:4892964/133‑1 (MQ=12)

                                                    ttGGCACCCAACCAGGACTGATCCGGCCAAATGGTGGGAGCGGGCGTACCCGCGACACATGCACCGCAGTCCATAACACCGTCTTTTCCGGTG‑T‑TAA‑AGGACAT‑ACCCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                            <  2:2015233/137‑1 (MQ=255)

                                                    ttGGCACCACACCAGGACTGAGGCGGCAAAACGGGGGGAGGGGGCGTGCCCGAGAAAGAGGTGCCGTGGGGCATGGCACCGGTTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                            <  2:6652756/137‑1 (MQ=16)

                                                       acaccacAGAGGGATGGAGGCGTCCAAAAGGGGGGAGGGGGGGGGCCCGTAAAACGGGAGCCGTGGGGAATGGCATTGGTTTTGCGGGGG‑TGC‑G‑TGGACAG‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                         <  2:2542474/136‑1 (MQ=255)

                                                        caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGTCCGCGAAAAAAGCGCCACGGTGCCTGGCACCAGCTTTTTCGGTG‑T‑TCG‑CGGGTCC‑GCCCCCTCCCACAAAATGATTGCCACTCAACGGGACt                                                                        >  1:7001934/1‑137 (MQ=255)

                                                        caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGTCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCt                                                                        >  2:6352587/1‑137 (MQ=21)

                                                        caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCCTGGCACCAGATTTGCCGGTG‑T‑TCG‑CAGGCAC‑GCCCACTCCCACAGAATGATTGCCATTCAACGGGCCt                                                                        >  1:10451558/1‑137 (MQ=255)

                                                        caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGTCCGCGAAACAAGCGCCGCGGTGTACGGCACCGGATTTGCCAGTGTT‑T‑T‑CGGGTAC‑GCCCACCCCCACAAAATGAGTGCCATTGAGAGGGCCt                                                                        >  1:7288381/1‑137 (MQ=255)

                                                          ccacAGCAGGACTGAGGCTGCCAAATGGTGGGAGTGGGTGTACCTGCGAAACAGGCACCGCGGTGCGTGGCACCGGTTTTGCCGGTG‑T‑TCG‑TGGACAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAt                                                                      <  1:13297130/137‑1 (MQ=255)

                                                            acaGCAGGACTGAGTCTTCCAAACGGTGGGGGCGGGCGTGCCCGCGAAACAGTCGCCGCGGTGCAGGGCACTGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg                                                                    <  2:11701272/137‑1 (MQ=18)

                                                                    aCTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCGAAACAAGCGCCACGGTGCCTGGCACCAGCCTTTTCAGTG‑T‑TCG‑CAGGCAC‑GCCCGCTCCCACAAAATGATTGCCACTCAGCGGGACCATCGCTCCAATg                                                            >  2:3006359/1‑137 (MQ=12)

                                                                    aCTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCATGGCACCGGATTTGCCGGTG‑T‑T‑TGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTCAGCGGGACTATCGCTACAGTg                                                            >  2:7547334/1‑137 (MQ=21)

                                                                     cTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCGGCTTTGCCGGTG‑T‑TCG‑AGGGCACAA‑CCGCTCCCACAGAATGATTGCCACTCAGCGGGACTACCGATAAAGGGa                                                           >  2:5066090/1‑137 (MQ=12)

                                                                      tGAGGCTACCAACCGGGGGGAGTGGGCGTACCCGCGAAACAGGCGCCGCGGTGCATGACACCGGCTTTGCTGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc                                                          <  2:13798245/137‑1 (MQ=21)

                                                                        aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCCCCTGCTTTGTCGGTG‑T‑TCG‑CGGGCCC‑GCCCGCTCCCAAAGACTGATTGCCATTAAGCGGGTCTACCGCTACAGTGACgg                                                        >  2:10411353/1‑137 (MQ=21)

                                                                          gttGCCAAACGGGGGGGGCGGGCGTTCCTGTGAAACGTGCGCCGCGGTGCGTGGCACCGGTTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcc                                                      <  2:11912032/135‑1 (MQ=18)

                                                                           cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCATGGCACCGGGTTTGCCAGTG‑T‑T‑TGCGGGACC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                     >  2:1680656/1‑137 (MQ=21)

                                                                           cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGTGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑TGTGGGGAC‑GCCCGCTCCCACAGGCTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAAGGccc                                                     >  2:8942531/1‑137 (MQ=21)

                                                                           cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCACGGTGCACGGCACCAGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                     >  2:15911107/1‑137 (MQ=21)

                                                                           cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGAGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAAAACGATTGCCATTAAACGGGCCTATCGCTACAGTGACGGccc                                                     >  2:7192611/1‑137 (MQ=18)

                                                                           cTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAAGCACCGCGGTGCATGGCACCGGATTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCAAAGAATGATTGCAATTAAGCGGGCCGACCACTCAAGTGACGGacc                                                     >  2:6453055/1‑137 (MQ=9)

                                                                           cTGCCAAACGGTGGGAGCGGGAGTGCCCGTGAAACAGGCGCCGCGGTGCATGGCACCGGTTTTGTCGGTG‑T‑TCG‑CGGGCAC‑GACCGCTCCCAAAGACTGAGTGCCATTAAGCGGGACTCGCACTACAGTGACGGacc                                                     >  2:5531945/1‑137 (MQ=255)

                                                                             gCCAAACGGTGGGAGCGGGCGTGCCCACAAAACAAGCGCCACAGTGCACGGCCCCAGCTTTTCCCGTG‑T‑TCT‑CGGGCACAC‑CCACCCCCCCAAAACGATTGCCACTCAACGGGCCCATCACTCCAATGACAGcccat                                                   >  2:4380961/1‑135 (MQ=255)

                                                                                aaaCGGTGGGAGCGGGCGTGCCCGCAAAACAAGAGCCGCGGTGCACGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCCCCGACTGATTGCCATTCAGGGGGACTATCGCCCCCGTGACGGCCCGtttt                                                >  2:5872676/1‑137 (MQ=9)

                                                                                 aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGTATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTCAGCGGGCCTCTCGCTACAGTGACGGCCCGttttt                                               >  2:15598995/1‑137 (MQ=18)

                                                                                 aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGAAC‑GCCCACTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt                                               >  2:8667077/1‑137 (MQ=21)

                                                                                      tGGGAGCGGGTGTGCCCGCGAAACGGGCGCCGTGGTGCAGGGCACCGGCTTTGCTGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg                                          <  2:3317264/137‑1 (MQ=21)

                                                                                           gCGGGGGTGCCCGCGGAACAGGCGCCACGGTGCATGGAACCGGCTTTGGCGGTG‑T‑TCG‑CGGGAAC‑GCCCGATCCAACAGGCTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCAATTTTCTGTTGCCTCg                                     >  2:407127/1‑137 (MQ=255)

                                                                                            cGGGCTTGCCCGCGAAACAGGTGCCGTGGTGCGTGGCATCGGCTTTGCCGGTG‑T‑T‑TGAGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc                                    <  2:14577654/137‑1 (MQ=25)

                                                                                            agggCGTACCAGAGAAACAGGCGCCGTGGTGCAGGGCACCGGTTTTGCTGGTG‑T‑TCG‑CGGACAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc                                    <  1:9071084/136‑1 (MQ=255)

                                                                                                      ctcgAAAAAGGCGCCGTGTTGCTTTGCATCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                          <  2:9073355/135‑1 (MQ=25)

                                                                                                      cgcgAAACAGGCGCTGCGGTGCATGGCACCGGCTTTGCCGGGG‑T‑TCG‑CGGGAAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCTTTTTTTGTTGCCTCGCTAACGAATCa                          <  2:10674676/137‑1 (MQ=25)

                                                                                                      cgcgAAACAGGCGCAGCGGTGCATGGCACTGGTTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCAACAGACTGATTGCCATTAAGCGGGCCTATCGTTATAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                          <  2:13179536/137‑1 (MQ=25)

                                                                                                      cgcaAAACATTCGCCGCGGTGCGTGCCCCCGTCTTTTCCGTTG‑T‑T‑CGCGGACAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                          <  1:4407140/137‑1 (MQ=255)

                                                                                                      cgaaaaaCAGGCGCCGTGGTGCGGGGCACTGGTTTTGCCGGTG‑T‑T‑TGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                          <  1:200421/133‑1 (MQ=37)

                                                                                                      cgaaaaaCAGGCGCCGTGGTGCATGGCACCGTCTTTGCTGGTG‑T‑TCG‑CGGACAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGATAACGAATCa                          <  1:14170624/133‑1 (MQ=255)

                                                                                                           aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCAAAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGACTCATGACt                     >  2:12225911/1‑137 (MQ=25)

                                                                                                                 cgcttcgGTGCATGGCACAAGCTTTGCTGGTG‑G‑TCG‑TGGGCAC‑GCCCGCTCCCACAGACTGATCGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCGCGCTAACGAATCATGACTCCCTTa               <  2:9145510/132‑1 (MQ=25)

                                                                                                                 cgccgtGGTGCGGGGCACCGGTTTGGCCGGTG‑T‑T‑TGTGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa               <  1:3796010/137‑1 (MQ=255)

                                                                                                                 cgccggGTTGCGTGGCACCGGTTTTTCGGGTG‑T‑T‑CGCGGGCAT‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa               <  2:6252241/137‑1 (MQ=25)

                                                                                                                 cgccgcGGTGCGTGGCACCGGCTTTGCTGGTT‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa               <  2:1061534/137‑1 (MQ=37)

                                                                                                                 cgcagcGGTGCATGGCACTGTCTTTGCGGTTG‑TCC‑G‑CGGACAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa               <  2:8218430/137‑1 (MQ=255)

                                                                                                                   ccgtGGTGCATGGCGCTGTTTTTGCTGGTG‑T‑T‑TGGGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGa             <  1:10483162/137‑1 (MQ=255)

                                                                                                                      cgGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg          >  1:3694123/1‑137 (MQ=37)

                                                                                                                      cgGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg          >  2:13207512/1‑137 (MQ=37)

                                                                                                                          gCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt      >  1:6109434/1‑137 (MQ=37)

                                                                                                                           cATGGCACCGGCTTTTCTGGGG‑T‑TCT‑CGGGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc     <  2:2669011/137‑1 (MQ=255)

||

GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑T‑T‑T‑CGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG  >  AE015451_phaM1/1499351‑1499611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑TT‑T‑CG‑‑GGCAC‑‑‑G‑‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTGAAA  >  AE015451_phaM1/1499335‑1499622

TTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGC                                                                                                                                                          <  LH00941:114:22FVMWLT1:2:1145:23539:13746/151‑1 (MQ=60)

 TGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCT                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1212:19453:15860/151‑1 (MQ=60)

 TGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCT                                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1262:24353:29022/151‑1 (MQ=60)

      ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGTAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC                                                                                                                                                    <  LH00941:114:22FVMWLT1:2:2106:14757:28446/151‑1 (MQ=60)

      ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC                                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1209:31832:2649/151‑1 (MQ=60)

      ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC                                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1160:43509:3402/151‑1 (MQ=60)

      ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC                                                                                                                                                    <  LH00941:114:22FVMWLT1:2:2239:41613:8510/151‑1 (MQ=60)

      ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC                                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1137:29308:14707/151‑1 (MQ=60)

      ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC                                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1244:26304:22601/151‑1 (MQ=60)

      ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC                                                                                                                                                    <  LH00941:114:22FVMWLT1:2:2208:23271:19143/151‑1 (MQ=60)

      ACGGGCCGCAGTTCGACAACGCCGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACATGCGCCGCGGTGCATGGCACCGGCTTTGCC                                                                                                                                                    <  LH00941:114:22FVMWLT1:2:2256:42815:5083/151‑1 (MQ=60)

          GCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGG                                                                                                                                                  <  LH00941:114:22FVMWLT1:2:1233:41835:29038/151‑1 (MQ=60)

          GCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1162:43545:13490/151‑1 (MQ=60)

          GCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1206:25092:8558/151‑1 (MQ=60)

              CAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCACGAAACAAGCGCCACGGTGCCTGGCACCGGCTTTGCCTGTG‑TTct                                                                                                                                           >  LH00941:114:22FVMWLT1:2:2155:39764:21801/1‑149 (MQ=60)

                GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcg                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1143:47280:3914/151‑5 (MQ=60)

                 TTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACATGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcgg                                                                                                                                        <  LH00941:114:22FVMWLT1:2:2114:42223:19687/151‑6 (MQ=60)

                  TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGANTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcggg                                                                                                                                       <  LH00941:114:22FVMWLT1:2:2232:42797:20039/151‑7 (MQ=60)

                  TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGANTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcggg                                                                                                                                       <  LH00941:114:22FVMWLT1:2:2232:42806:20023/151‑7 (MQ=60)

                  TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGccatgcac                                                                                                                                        <  LH00941:114:22FVMWLT1:2:1212:18815:2457/151‑9 (MQ=60)

                  TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcggg                                                                                                                                       <  LH00941:114:22FVMWLT1:2:1167:24760:5868/151‑7 (MQ=60)

                  TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcggg                                                                                                                                       <  LH00941:114:22FVMWLT1:2:2237:40411:10624/151‑7 (MQ=60)

                   CGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcgggc                                                                                                                                      <  LH00941:114:22FVMWLT1:2:2253:30713:22649/151‑8 (MQ=60)

                         CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTcgcgggcacgccc                                                                                                                                <  LH00941:114:22FVMWLT1:2:2117:49999:20343/151‑14 (MQ=60)

                          GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGGCCGCGAAACAAGAGCCACGGTGCCTGGCCCCGGGTTTTCCGGTGTTT‑T‑CG‑‑GGCAC‑‑‑GA‑‑CCG                                                                                                                       >  LH00941:114:22FVMWLT1:2:1210:27598:21160/1‑151 (MQ=60)

                                GCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCACGGTGCATGGCACCGGATTTGCCGGTG‑TTcgcgggaacgcccgctcccc                                                                                                                         >  LH00941:114:22FVMWLT1:2:1170:38655:21288/1‑131 (MQ=60)

                                 CCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAAAAGGAGCCGCGGTGTATGGAACCGGCTTTGTCGGTG‑TT‑TGCG‑‑GGCAC‑‑‑GA‑‑CCGCTCCCAC                                                                                                                >  LH00941:114:22FVMWLT1:2:2202:46541:19351/1‑151 (MQ=60)

                                       ATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGTGCCGCGGTGTATGGCACCTGCTTTGCCGGTG‑TTctcgggcccgcccacccccaaaaactg                                                                                                                  >  LH00941:114:22FVMWLT1:2:2265:11613:15219/1‑124 (MQ=60)

                                            GCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAAAAGGCGCCACGGTGCCTGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAAACTGATTGC                                                                                                     >  LH00941:114:22FVMWLT1:2:2225:5992:24603/1‑151 (MQ=60)

                                               ATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGAGCCACGGTGCATGGCACCGGCCTTGCCGGTG‑TT‑Tgcaggaccgccccctcccacagaatgggtgccct                                                                                                         >  LH00941:114:22FVMWLT1:2:1235:15080:15075/1‑117 (MQ=60)

                                                TTCGGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCCCGAAACAGGAGCCACGGTGTCTGGCCCCAGCTTTGCCGGTG‑TT‑Tgcgggcccacccactcccccagaccggttgccact                                                                                                        >  LH00941:114:22FVMWLT1:2:2215:42418:16564/1‑116 (MQ=60)

                                                TTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCAAAACAGGCGCCGCGGGGCAGGGCACCGGCTTTGTCGGTG‑TTcgcgggtacgcccacccccacaaaccaattgccact                                                                                                         >  LH00941:114:22FVMWLT1:2:2244:16365:13970/1‑115 (MQ=60)

                                                TTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCACGAAACAAGCGCCGCGGTGCATGGCACCGGATTTGCCGGTG‑TTcgcggggaagcccgctcccacagaatgagtgacatt                                                                                                         >  LH00941:114:22FVMWLT1:2:1241:11604:19687/1‑115 (MQ=60)

                                                TTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAAGCGCCGCAGTGCATGGCACCAGCTTTGCCGGTG‑TTcgcgggcacgcccacccccaccgaatgattgccact                                                                                                         >  LH00941:114:22FVMWLT1:2:2164:18972:17045/1‑115 (MQ=60)

                                                          GAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCCTGGCACCAGATTTGCCGGTG‑‑T‑T‑CGCAGGCAC‑‑‑GC‑‑CCACTCCCACAGAATGATTGCCATTCAACGGGCCT                                                                                       >  LH00941:114:22FVMWLT1:2:2134:44442:25452/1‑151 (MQ=60)

                                                                     TGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCGCGAAAAAGGTGCCGCGGTGCATGGCCccagttttgtcggtgttcgcgggaaagcccgatcccaaagaatgattggcattcagcgggactactgatgccgt                                                                                     >  LH00941:114:22FVMWLT1:2:1114:36140:26092/1‑77 (MQ=60)

                                                                       C‑‑CACCACAGAAGGACTGAGGCGGCCAAGTGGTGGGAGCGGGCGTGCCCGCAAAACAGGCACCGCGGGGCATGGCACCGTTTTTGCCGGT‑‑TT‑T‑CG‑‑GGGGC‑‑‑GCTCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGA                                                                          <  LH00941:114:22FVMWLT1:2:2161:3394:7485/151‑1 (MQ=60)

                                                                            CCACAGCAGGACTGAGGCTGCCAAATGGTGGGAGTGGGTGTACCTGCGAAACAGGCACCGCGGTGCGTGGCACCGGTTTTGCCGGTG‑‑T‑T‑CG‑‑TGGACACTC‑‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGG                                                                       <  LH00941:114:22FVMWLT1:2:2212:22448:3690/151‑1 (MQ=60)

                                                                                    GGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCTAGAAACAAGAGCCGCGGTGCATGGCACCGGCTTTTCCGGTG‑TTCT‑CG‑‑GGCACacccgctcccccaaaccggtttccattgagcgggtccctcgctacaatggagggccgtttt                                                                    >  LH00941:114:22FVMWLT1:2:2138:51681:12721/1‑90 (MQ=19)

                                                                                                               GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAACCATGACT                                    >  LH00941:114:22FVMWLT1:2:1208:37000:27133/1‑151 (MQ=60)

                                                                                                               GGGCGTGCCCGCAAAACAGGCACCGCGGTGCATGGCACCGGCTTTGTCGGTG‑TTCG‑CG‑‑GGAAC‑‑‑GC‑‑CCGCTCCCAAAGACTGATTGCACTTAAGCAGGCCTATCGCTACCATGACAGACCGTTTTTTGTTGCCTCGCTACCGAACCATGACT                                    >  LH00941:114:22FVMWLT1:2:2245:40809:18422/1‑151 (MQ=18)

                                                                                                                      CCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑G                         >  LH00941:114:22FVMWLT1:2:1241:14378:26252/1‑151 (MQ=60)

                                                                                                                        gatgtgtataagagacaGGTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAA                       <  LH00941:114:22FVMWLT1:2:2157:26211:6556/134‑1 (MQ=60)

                                                                                                                         GCGAAACGGGCGCTGCGGTGCAGTGCACCGGTTTTTCCGGTG‑TTCT‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAAC                      <  LH00941:114:22FVMWLT1:2:1267:18279:24811/151‑1 (MQ=60)

                                                                                                                          CGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACG                     >  LH00941:114:22FVMWLT1:2:1162:50267:19943/1‑151 (MQ=60)

                                                                                                                          CGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACG                     >  LH00941:114:22FVMWLT1:2:1162:50257:19927/1‑151 (MQ=60)

                                                                                                                          CGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACG                     >  LH00941:114:22FVMWLT1:2:1157:16393:15844/1‑151 (MQ=60)

                                                                                                                              ACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTAT                 >  LH00941:114:22FVMWLT1:2:1232:7915:20920/1‑151 (MQ=60)

                                                                                                                                  GCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAG             >  LH00941:114:22FVMWLT1:2:2222:48094:7757/1‑151 (MQ=60)

                                                                                                                                  GCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAG             >  LH00941:114:22FVMWLT1:2:2252:42778:27469/1‑151 (MQ=60)

                                                                                                                                     agcCGGTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACtgtctcttatac          >  LH00941:114:22FVMWLT1:2:2120:31213:6508/4‑139 (MQ=60)

                                                                                                                                     CCGCGGTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAG          >  LH00941:114:22FVMWLT1:2:2153:38211:19975/1‑151 (MQ=60)

                                                                                                                                       GCGGTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGAT        >  LH00941:114:22FVMWLT1:2:1228:2710:2105/1‑151 (MQ=60)

                                                                                                                                       GCGGTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTANCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGAT        >  LH00941:114:22FVMWLT1:2:2249:16134:3834/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTTCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGACCCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1242:26341:16196/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTCTCGCTACAGTAACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1223:28051:14675/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGCATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1235:6473:27677/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACtgtctcttatacacatc     >  LH00941:114:22FVMWLT1:2:1116:50100:9086/1‑134 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2207:4032:16148/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2218:20803:8910/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2245:33598:1737/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2207:4023:16132/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2206:9931:14835/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1105:9126:10656/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2165:19518:4923/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2165:19508:4907/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2144:12307:9343/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2141:24778:28958/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2123:50063:27821/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1105:6787:8590/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1106:40744:12545/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1270:14230:11328/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1106:40753:12561/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1207:47003:10896/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1108:39505:20968/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2203:15977:9006/1‑151 (MQ=60)

                                                                                                                                          GTGCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1140:13000:13010/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGAACGCTATCAAGCAGATC       >  LH00941:114:22FVMWLT1:2:2250:30159:5035/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:1151:51912:16196/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:2256:25444:19447/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:1137:24066:1849/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:1168:9311:1528/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:1231:6695:9423/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:1259:7277:29391/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:2107:11826:29327/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:2123:46227:27773/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACAAATCATGACTCCCTTAGAACGAACGCTATCAAGCAGATC       >  LH00941:114:22FVMWLT1:2:2250:30149:5051/1‑151 (MQ=60)

                                                                                                                                            GCATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTAATTGCCATCAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:2269:25277:4010/1‑151 (MQ=60)

                                                                                                                                             CATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTGAAA  >  LH00941:114:22FVMWLT1:2:1125:16014:15956/1‑151 (MQ=60)

                                                                                                                                             CATGGCACCGGCTTTGCCGGTG‑TTCG‑CG‑‑GGCAC‑‑‑GC‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTGAAA  >  LH00941:114:22FVMWLT1:2:1112:42612:4219/1‑151 (MQ=60)

TTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGG‑‑CACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑TT‑T‑CG‑‑GGCAC‑‑‑G‑‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTA‑‑‑‑GAACGCTATCAAGCAGATCTGAAA  >  AE015451_phaM1/1499335‑1499622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`