Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F11 I3 R1	74	850.3	37733610	99.3%	37469474	136.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	1,499,480	2 bp→AC	intergenic (+57/‑106)	trpS CDS (Tryptophan‑‑tRNA ligase) → / → zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	1,499,480	0	C	A	95.2%	449.9 / NA	207	intergenic (+57/‑107)	trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain)	–/–
Reads supporting (aligned to +/- strand): ref base C (10/0); new base A (101/96); total (111/96)
Fisher's exact test for biased strand distribution p-value = 1.97e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	AE015451_phaM1	1,499,481	0	A	C	97.5%	480.2 / NA	207	intergenic (+58/‑106)	trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain)	–/–
Reads supporting (aligned to +/- strand): ref base A (4/0); major base C (107/91); minor base T (0/5); total (111/96)
Fisher's exact test for biased strand distribution p-value = 2.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

TTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑T‑T‑T‑CGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATC  >  AE015451_phaM1/1499352‑1499608

||

ttCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCttt                                                                                                                                <  2:5230731/137‑1 (MQ=255)

 tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg                                                                                                                               <  2:10421838/137‑1 (MQ=255)

 tCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTg                                                                                                                               <  1:9372552/137‑1 (MQ=255)

   gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGcc                                                                                                                             <  2:6937/137‑1 (MQ=255)

     cAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCgg                                                                                                                           <  2:4894192/137‑1 (MQ=255)

       aCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtg                                                                                                                         <  2:1069215/137‑1 (MQ=255)

        cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTTGCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtg‑t                                                                                                                       <  2:4264074/137‑1 (MQ=38)

           tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGTCCGCGAAACAGGCGCCGCGGTGCATGGCCCCGGCTTTGCCGGTG‑t‑tcg                                                                                                                   >  2:10847414/1‑135 (MQ=255)

           tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCGCGAAACAAGAGCCACGGTGCATGGCCCCGGCTTTTGCAGTG‑t‑t‑tt                                                                                                                  >  1:10837755/1‑136 (MQ=255)

           tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGATGACCACGAAACAAGCACCACAGTGCATGGCACCGACTTTGTCCGTT‑T‑T‑T‑c                                                                                                                 >  1:1138912/1‑137 (MQ=255)

             aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCGCGAAACAAGCGCCACGGTGCACGGCACCAGCTTTGCCGGTG‑t‑tcgcg                                                                                                                 >  1:11774809/1‑133 (MQ=255)

             aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCACGAAACAGGCGCCGCGGTGTATGGCACCGGCTTTGCCGGTG‑t‑tcgcg                                                                                                                 >  2:15529424/1‑133 (MQ=38)

             aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGGGAAACAGGGGCCGCGGTGTATGGCACCGGCTTTTCCGGTG‑t‑t‑tgcg                                                                                                                >  1:13578118/1‑134 (MQ=38)

                     gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCACGGTGCCCGGCACCGGCTTTGCCGGTG‑T‑TCG‑CAGGCAc‑g‑cc                                                                                                      >  2:8178206/1‑137 (MQ=25)

                     gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCACGGTGCATGGCACCGGCTTTGTCGGTG‑T‑TCG‑CGGGCAc‑g‑cc                                                                                                      >  2:254030/1‑137 (MQ=25)

                     gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAGGCACCGCAGTGTATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAc‑g‑cc                                                                                                      >  1:12830544/1‑137 (MQ=25)

                       tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCCCGAAACAAGAGCCACAGTGCATGGCCCCCGCTTTTTCTGTG‑T‑TCT‑CGGGAAc‑g‑cccc                                                                                                    >  1:1591178/1‑135 (MQ=255)

                       tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAAACGCCACGGTGCCCGGCACCCGCTTTTTCTGTGTT‑T‑T‑CGGGCAc‑a‑cccc                                                                                                    >  1:7210195/1‑135 (MQ=255)

                       tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAc‑g‑cccg                                                                                                    >  2:8600903/1‑135 (MQ=25)

                           gCCATTCAGAGCTTGAAAGAAGCTTTGGCACCACAGCGGGATGGGGGCTGACAAGCGGGGGGAGTGGGCGTTCCCGAGAAAAGGGCGCCGCGGTGCATGGCACCGGCTTTGCTGGTG‑T‑TCG‑CGGGCAC‑TCCCGCTcc                                                                                                 <  1:9136802/137‑1 (MQ=12)

                            ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTccc                                                                                                >  2:2146854/1‑137 (MQ=21)

                            ccATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTTCACGGCACCGGCTTTGCCAGTG‑T‑TCG‑CAGGCAC‑GCCCGCTccc                                                                                                >  2:10757387/1‑137 (MQ=18)

                              aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCcac                                                                                              >  2:8386896/1‑137 (MQ=21)

                              aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCATGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCcac                                                                                              >  2:11755414/1‑137 (MQ=21)

                                 cAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCAAGAAACAGGAGCCGCGGGGCATGGCACCGGATTTGCCAGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGa                                                                                           >  1:2601955/1‑137 (MQ=21)

                                       cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCCCCGGGTTTGTCGGTG‑T‑TCG‑CGGGCCC‑GTCCGCTCCCACAGACTGGtt                                                                                     >  2:7634847/1‑137 (MQ=21)

                                         gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAA‑GCCCGCTCCCACAGACCGATTGc                                                                                   >  2:5207217/1‑137 (MQ=18)

                                         gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCACGGCACCGGCTTTGCCAGTG‑T‑TCG‑CGGGCAC‑GCCCACTCCCACAGAATGATTGc                                                                                   >  2:16211417/1‑137 (MQ=18)

                                         gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAACAGGAGCCGCAGTGTATGGAACCGGATTTGCCAGTG‑T‑TCG‑CAGGCAC‑GCCCCCTCCCACAGAATGGTTga                                                                                   >  1:14015697/1‑136 (MQ=16)

                                         gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAAAAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCACTCCCACAGACTGATTGc                                                                                   >  2:15649237/1‑137 (MQ=21)

                                         gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGACCGCGAAAAAGGCACCGCGGTGCATGGCACCAGCTTTGGCGGTT‑T‑TCA‑CGGGCCC‑GCCCGATCCCACAGGCTGATTGc                                                                                   >  2:11411147/1‑137 (MQ=255)

                                             gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCAGCTTTTCCGGTG‑T‑TCT‑CGGGCACAC‑CCACTCCCCCAAACCGATTTCCAtt                                                                               >  1:8251449/1‑137 (MQ=255)

                                             gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGCCCACGAAACAAGAGCCGCGGTGTATGGCACCAGCCTTTCCTGTGTT‑T‑T‑CGGGCACAC‑CCACTCCCAAAAACTGATTGCCAtt                                                                               >  1:3842728/1‑137 (MQ=255)

                                             gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGGCCGCGAAACAAGAGCCGCGGTGTATTGCACCAGCTTTGCCGGTG‑T‑T‑TGCGGGACC‑GCCCACTCCCACAGAATGAGTGACAtt                                                                               >  2:14493285/1‑137 (MQ=255)

                                             gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAAACTGATTGCCAtt                                                                               >  2:17476067/1‑137 (MQ=21)

                                             gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCACGGTGCACTGCACCAGCTTTTCCGGTG‑T‑TCG‑CGGGCAC‑GCCCCCTCCCACAAAATGATTGCCAtt                                                                               >  2:8098455/1‑137 (MQ=18)

                                             gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGAGCCGAGGTGCACGGCAACAGCTTTGCCGGTG‑T‑TCG‑TGGGCAC‑GCCCGCTCCCACAGAATGAGTGCCAtt                                                                               >  2:14394106/1‑137 (MQ=18)

                                             gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAAAAAGCGCCGCGGTGCATGGCACCGGCTTTTCCGGTG‑T‑T‑TGCGGGCACAC‑CCGCTCCCACAGAATGATTGCCAtt                                                                               >  2:12625641/1‑137 (MQ=18)

                                             gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGTCCGCAAAACAGGAGCCACGGTGCATGGCACCGGCTTTTCCGGTGTT‑T‑T‑CGGGCAC‑GCCCACTCCCACCGACGGATTGCCCtt                                                                               >  1:17232763/1‑137 (MQ=21)

                                             gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAAGAGCCACAGTGCACGGCACCAGATTTGCCAGTG‑T‑T‑TGCGGGCAC‑GCCCACTCCCACAAAATGATTGCCact                                                                               >  2:6169870/1‑135 (MQ=255)

                                             gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCACGAAACAAGCACCACGGTGTCTGGCACCAACTTTTCCGGTGTT‑T‑T‑TGGGCACAC‑CCACTCCCCCAAAATGATTGCCAtt                                                                               >  1:5430246/1‑137 (MQ=255)

                                                 ccTTGACCCCACAACAGGCCTGAGGCTGCCAAACGGTGGGGAGGGGCGTACCCGCAAAAAGTACCCCGCGGTACGTAACACCGGCTTGGCTGGGG‑TCA‑G‑TGGGCAC‑GCCCGCTCCCACAGACTGATTACCATTAAGc                                                                           <  2:3107489/137‑1 (MQ=255)

                                                   ttGGCACCACAGCGGGACTGAGGCTGCAAAAAGGTGGGAGCGGGTGTGCCAGAGAAACGGGCGCTGTGGTGCGGGGCACCGGTTTTGCTGGTG‑T‑T‑CGCGGGAAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                         <  2:10918848/137‑1 (MQ=255)

                                                   ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGATTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGAATGATTGCCATTAAGCgg                                                                         >  2:1846857/1‑137 (MQ=21)

                                                   ttGGAACCACAGCAGGACTGAGGTTGCCAACCGGTGGGAGTGGGCGTACCCGCAAAACAGGCGCCGTGGTGCATGGCACTGGTTTTGCCGGTG‑T‑T‑TGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                         <  2:17361721/137‑1 (MQ=21)

                                                   tgggCACCACAGCAGGACTGAGGCTGCCAAATTGTGGGAGCGGGCGTGCCTGCGAAACAGTCGCCGCGGTGCGTGGCACCGGTTTTGCAGTTG‑T‑TCG‑TGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                         <  2:3475749/135‑1 (MQ=18)

                                                    tGGCACCACAGCGGGATTGAGGTTGCCAAATGGTGGGAGCGGTTGTTCCCGCGAAACGGGCGCCGTGGGGCAGGGAACTGGCTTTGCTGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCggg                                                                        <  2:1833063/137‑1 (MQ=16)

                                                      gaaccacAGAAGGATGGGGGCTACAAAATGGGGGGAGCGGGTGTTCCCGAGAAACAGGCGCCGTGGGGCGTGGCACTGGTTTTTCCGGTT‑T‑TCG‑CGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                      <  1:9341229/135‑1 (MQ=255)

                                                      gCACCACAGCAGTACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACGGGCGCCGTGGTACATGGCACTGGCTTTTCCGGTG‑T‑TCG‑CGGGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                      <  2:11886665/137‑1 (MQ=18)

                                                      gCACCACAGCAGGATTGAGGCTGCAAAATGGTGGGGGCGGGTGTGCCCGCGAAAAGGGCGCCGCGGTGCATGGCACCGGTTTTTCTGTTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                      <  2:8899140/137‑1 (MQ=16)

                                                      gCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGAGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                      <  2:16713815/137‑1 (MQ=21)

                                                      gCACCACAGAAGGGTGGGTGCGGCAAAATGGGGGGAGTGGGAGTTCCCGAGAAACAGGCGCCGGGGGGTGTGTCACTGGTTTTGTTGTTG‑T‑T‑CGTGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                      <  1:2070509/137‑1 (MQ=255)

                                                      gCACCACAGAAGGATTGAGGCTGCAAACCGGTGGGAGGGGGCGTTCCCGAGAAAAGGGCGCCACGGGGCGGGGCACTGGTTTTGCTGGGG‑T‑T‑TGCGGGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                      <  1:12384849/137‑1 (MQ=255)

                                                      acaccccAGCAGGACTGGGTTTGCCAAACGTGGGGATCGGTTTTCCCTGCGAAACGGGCGCCGTGGTGCGTGCCACCGTTTTTACCGGTG‑T‑TCG‑CGGGCAC‑ACCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                      <  1:6619821/136‑1 (MQ=255)

                                                      acaccacAGAAGGGTGGAGGTTGCAAAATGGGGGGAGGGGGTGTTCACGAAAAAAGTGCGCCGTGGGGCAGGGCACTGGTTTTGCGGGTG‑T‑TCG‑TGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                      <  2:5584688/136‑1 (MQ=255)

                                                      aaacaacaGAAGGATGGGGGCGGCAAAATGGGGGGGGGGGGGGGGCCCGCGAAAAGGGCGCGGTGGTGAGGGGCATTGGTTTTGCGGGTG‑T‑TCG‑TGGACAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc                                                                      <  1:3337782/132‑1 (MQ=255)

                                                              gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCACGGTGCCTGGCACCGGCTTTGTCGGTG‑T‑TCG‑AAGGCACAC‑CCGCTCCCACAGGATGATTGACATTAAGCGGGACTATCGCTa                                                              >  2:1312640/1‑137 (MQ=18)

                                                              gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAAGCGCCACGGTGCATGGCACCGGCTTTGTCGGTG‑T‑T‑TACGGGCAC‑GCCCGCTCCCACAGACCGATTGCCATTAAACGGGACTATCGccc                                                              >  2:1020820/1‑135 (MQ=18)

                                                                   aCTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCACAAAACAAGAGCCACGGTGTATGGCACCAGATTTTTCGGTG‑T‑TCA‑CGGGCACAC‑CCACCCCCCCAAACTGATTGCCATTAAACGGGCCCATCGCTGCAGTg                                                         >  1:4112236/1‑137 (MQ=255)

                                                                     tGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAGGCGCCACAGTGCACGGCACCAGCTTTGCCGGTG‑T‑T‑TGCGGGCACAA‑CCGCTTCCACAGACTGATTGCCACTCAGCAGGCCTACCACTACAATGAc                                                       >  2:1346109/1‑137 (MQ=255)

                                                                     ggAGGCTGCAAAATGGTGGGGGGGGGTGTGCCCGAAAAACGGGCGCCGCGGTGCATGGCATTGGTTTTTCTGGTG‑T‑T‑CGAGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc                                                       <  2:14789684/136‑1 (MQ=255)

                                                                      gAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCAGTTCACGGCACCGGCTTTGCCGGTT‑T‑TCG‑CGGGCCC‑GCCCGCTCCCACCGACTGATTGCCATTAAGCGGGCCTATCGCCACAGTGAca                                                      >  2:9880844/1‑136 (MQ=18)

                                                                          cTGCCAAACGGTGGGAGCGGGCGTTCCCGCGAAACAGACGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑TGGGCAC‑GCCCGCTCCCAAAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcac                                                  >  2:7252958/1‑135 (MQ=21)

                                                                          cTGCCAAACGGTGGGAGCGGGCGTGTCCGCGAAACAGGCGCCGCGGTGCCTGGCCCCGGCTTTGCCGGTG‑T‑T‑TGCGGGGAC‑GCCCGCTTCCACAAACTGATTGCCATTAAGCGGGCCTATTGCTACAGCGACGGccc                                                  >  2:6722324/1‑137 (MQ=37)

                                                                          cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGAATGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                  >  2:17104684/1‑137 (MQ=18)

                                                                          cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCACGGCACCGGATTTGCCAGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAAAATGATTGCAATTCAACGGGCCTATCGCCACAATGACGGccc                                                  >  2:12508491/1‑137 (MQ=17)

                                                                          cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAGGCGCCACGGTGCATGGCACCGGATTTGTCGGTG‑T‑T‑TGCGGGCACAC‑CCACTCCCACAGACTGGTTTCCCTTCAGCGGAACTGTTGCTGCAGTGAAGGgcc                                                  >  1:18470448/1‑137 (MQ=9)

                                                                               aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCCCAGACTGATTGCCCTTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttt                                             >  2:18772385/1‑137 (MQ=21)

                                                                               aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCACGtttt                                             >  2:9333810/1‑137 (MQ=21)

                                                                               aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTCAGCGGGCCTCTCGCCACAGTGACGGCCCGtttt                                             >  2:13888989/1‑137 (MQ=18)

                                                                               aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCATGGCCCCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑ACCCGCTCCCCCAGAATGATTGCCATTCAGCGGGCCTATCGCTACCGTGACGGACCGtttt                                             >  1:4223472/1‑137 (MQ=18)

                                                                               aaaCGGTGGGAGCGGGCGTGCCCGCAAAACAGGCGCCGCGGTGCCCGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCACAC‑CCGCTCCCAAAGAATGATTGCCATTAAGTGGGCCTATCGCTACAATGACGGCCCGtttt                                             >  2:2726313/1‑137 (MQ=18)

                                                                                 aCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACCGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt                                           >  2:15937897/1‑137 (MQ=18)

                                                                                   tttGGGAGCGGGCGTCCCCGCGAAATAGGCGTCGTGGGGCAGGGCACTGGTTTTGCTGGTG‑T‑TCG‑CGGGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt                                         <  1:5784409/135‑1 (MQ=255)

                                                                                        gAGCGGGCGTGCCCGCGAAACAAGCGTCCCAGTGCCTGGCCCCAGCTTTGTCCGTG‑T‑TCG‑CCGGCACAC‑CCGCTCCCACCAACTGATTGTCATTAAGCGGGCCTCCCTCTCCAGTGGCGGACCGTTTTTTGTTGCCt                                    >  2:400945/1‑137 (MQ=9)

                                                                                          gCGGGCGTGCCCACGAAAAAAGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTT‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTCAGCGGGCCTCTCGCTACAGTTGCGGCCCGTTTTTTGTTGCCTCg                                  >  2:18213356/1‑137 (MQ=18)

                                                                                            gggTGTGCCTGAGAAACAGGCGCCGCGGTGCGTGGCACCGGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCt                                <  2:16479936/137‑1 (MQ=25)

                                                                                                    ccGCGAAAAGGGCGCCGTGGTGCGTGGCATTGTTTTTGCGGGTG‑T‑TCG‑TGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc                        <  2:15865049/137‑1 (MQ=255)

                                                                                                    ccGCGAAAAAGGCGCCGGGGTGCGTGGAACCGGTTTTCCTGGTG‑T‑TCG‑TGGGAAC‑ACCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc                        <  2:1423918/137‑1 (MQ=255)

                                                                                                     tgcgAAACAGGTTCCGTGGGGCAGGGCACTGTTTTTGTGGGTG‑T‑T‑TGGGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:12828264/136‑1 (MQ=255)

                                                                                                     cgcgAAACGGGCGCCGCGGTGCATGGCACCGGTTTTTCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:6310582/137‑1 (MQ=25)

                                                                                                     cgcgAAACAGGTGCCGCGGTGCATGGCACCGTCTTTACCGTTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:1977617/137‑1 (MQ=37)

                                                                                                     cgcgAAACAGGCGCCGCGTTGCATGGCACCGGTTTTGCTGGTG‑T‑TCG‑AGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:15171002/137‑1 (MQ=25)

                                                                                                     cgcgAAACAGGCGCCGCGGTGCATGACACCGGCTTTTCCGTTG‑T‑TCG‑CGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:15655868/137‑1 (MQ=255)

                                                                                                     cgcaAAACGGGCGCCGCGGTGCATGCCACCGTTTTTTCCGTTG‑T‑TCT‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  1:18116023/137‑1 (MQ=255)

                                                                                                     cgagAAACGGGCTCCGTGGTGCATGGAACTGGTTTTGCTGGTG‑T‑T‑CGGGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  2:1234948/134‑1 (MQ=25)

                                                                                                     cgagAAACAGGTGCCGCGGTGAGGGGCACCGGTTTTGTTGGGG‑T‑T‑TGTGGGCAT‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  1:10226358/134‑1 (MQ=255)

                                                                                                     cacgAAACAGTCGCCGCGGTGCATGACACCGGCTTTGTCGTTT‑T‑CCG‑CGGGCAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  1:8593520/135‑1 (MQ=255)

                                                                                                     agcgAAACAGGCGCCGTGGTGCATGGCATCGGTTTTGCTGGTG‑T‑T‑TGCGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  1:12434153/136‑1 (MQ=255)

                                                                                                     agagAAACGGGCGCTGGGGTGCAGGGCACTGTTTTTGCTGGTG‑T‑TCG‑CGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa                       <  1:14902472/134‑1 (MQ=255)

                                                                                                      gcgAAACGGGCGCTGTGGGGCAGGGCACTGGCTTTGCTGGTT‑T‑TCG‑CGGGCAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                      <  1:15761641/137‑1 (MQ=255)

                                                                                                      gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                      <  2:17732192/137‑1 (MQ=25)

                                                                                                                cttcgcgGGGCAGGGCACTGGTTTTGCTGGTG‑T‑TCG‑CGGACAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa            <  1:10923403/134‑1 (MQ=255)

                                                                                                                ctccgcGGTGCATGGCACCGGCTTTTCCGGTG‑T‑T‑CGTGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa            <  2:6490289/135‑1 (MQ=37)

                                                                                                                ctccgcGGTACGTGGCACTGGCTCTGCCGGTT‑T‑TCG‑GGGGCACTC‑CCGCTCACACAGACTGAGTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa            <  1:5500727/135‑1 (MQ=255)

                                                                                                                cgccgtGGTGCGTGACACCGTCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa            <  2:7882999/137‑1 (MQ=37)

                                                                                                                cgccgcGGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa            >  2:10681648/1‑137 (MQ=37)

                                                                                                                caccgcGGTGCATGGCACCGGCTTGTCCGGGG‑T‑TCG‑TGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa            <  2:7414084/135‑1 (MQ=25)

                                                                                                                     cgGTGCATGGCACCTGCTTTGCCGGTG‑T‑T‑CGCGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg       <  2:13705471/137‑1 (MQ=37)

                                                                                                                     cgGTGCATGGCACCGGCTTTGCCGGTG‑T‑TCG‑CGGGCAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACg       <  2:17891435/137‑1 (MQ=37)

||

TTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTG‑T‑T‑T‑CGGGCAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATC  >  AE015451_phaM1/1499352‑1499608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGG‑TG‑TT‑‑T‑‑C‑‑GGGCA‑C‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAA  >  AE015451_phaM1/1499335‑1499622

TTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGC                                                                                                                                                      <  LH00941:114:22FVMWLT1:2:2126:51607:21689/151‑1 (MQ=60)

TTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGC                                                                                                                                                      <  LH00941:114:22FVMWLT1:2:1131:3515:26012/151‑1 (MQ=60)

 TGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCGTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCT                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:2101:14100:28046/151‑1 (MQ=60)

 TGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCT                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:2147:48871:8366/151‑1 (MQ=60)

 TGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCT                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1202:45616:20279/151‑1 (MQ=60)

      ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1142:20304:13618/151‑1 (MQ=60)

      ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2104:39847:7309/151‑1 (MQ=60)

      ACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2136:9015:3322/151‑1 (MQ=60)

         GGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGAGCCGCGGTGCATGGCACCGGCCTTGCCGG‑T                                                                                                                                            >  LH00941:114:22FVMWLT1:2:2134:23623:18534/1‑151 (MQ=60)

          GCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGG‑TG                                                                                                                                           <  LH00941:114:22FVMWLT1:2:1205:8322:2057/151‑1 (MQ=60)

          GCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGG‑TG                                                                                                                                           <  LH00941:114:22FVMWLT1:2:1244:41909:13826/151‑1 (MQ=60)

          GCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGG‑TG                                                                                                                                           <  LH00941:114:22FVMWLT1:2:2205:22985:3594/151‑1 (MQ=60)

              CAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCGCGAAACAAGAGCCACGGTGCATGGCCCCGGCTTTtgcagtgtttt                                                                                                                                        >  LH00941:114:22FVMWLT1:2:2121:6353:22665/1‑140 (MQ=60)

              CAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGG‑TG‑TTcg                                                                                                                                      <  LH00941:114:22FVMWLT1:2:1253:25601:25772/151‑3 (MQ=60)

                GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCGCGAAACAAGCGCCACGGTGCACGGCACCAGCTTTGCCGG‑TG‑TTcgcg                                                                                                                                    >  LH00941:114:22FVMWLT1:2:2135:6649:24203/1‑147 (MQ=60)

                GTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGGGAAACAGGGGCCGCGGTGTATGGCACCGGCTTTTCCGG‑TG‑TT‑‑Tgcg                                                                                                                                  >  LH00941:114:22FVMWLT1:2:2162:23576:23546/1‑148 (MQ=60)

                  TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGG‑TGccatgcac                                                                                                                                   <  LH00941:114:22FVMWLT1:2:1269:30149:19879/151‑9 (MQ=60)

                  TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGG‑TG‑TTcgcggg                                                                                                                                  <  LH00941:114:22FVMWLT1:2:2122:21052:2938/151‑7 (MQ=60)

                  TCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGG‑TG‑TTcgcggg                                                                                                                                  <  LH00941:114:22FVMWLT1:2:1222:39265:29679/151‑7 (MQ=60)

                        ACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGG‑TG‑TTcgcggggacgcc                                                                                                                            >  LH00941:114:22FVMWLT1:2:2151:41604:17621/1‑139 (MQ=60)

                        ACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAGGCACCGCAGTGTATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑G‑CC                                                                                                                    >  LH00941:114:22FVMWLT1:2:2151:41595:17637/1‑151 (MQ=60)

                          GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCACGGTGCCTGGCCCCGGCTTTtcctgtgttcgccggcccgccca                                                                                                                            >  LH00941:114:22FVMWLT1:2:2159:42260:13922/1‑128 (MQ=60)

                                 CCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGTCCGCGAAACAAGAGCCACGGGGCATGGCACCGGCTTTGCCGG‑TG‑TTcgcgggtacacccgctcccac                                                                                                                   >  LH00941:114:22FVMWLT1:2:2253:16707:23146/1‑130 (MQ=60)

                                  CCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAAGCGCCGCAGTGCCTGGCACCGGCTTTTCCGG‑TG‑TTcgtgggaacgcccactcccaca                                                                                                                  >  LH00941:114:22FVMWLT1:2:1157:18575:12833/1‑129 (MQ=60)

                                    GTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCAAGAAACAGGAGCCGCGGGGCATGGCACCGGATTTGCCAG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGA                                                                                                         >  LH00941:114:22FVMWLT1:2:1139:45200:20327/1‑151 (MQ=60)

                                                TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCGCGAAAAAGGTGCCGCGGTGTATGGCCCCGGCTTTGTCGG‑TG‑TT‑‑Tgtgggcccgcccgctcccacagaactattgaccct                                                                                                  >  LH00941:114:22FVMWLT1:2:1160:32747:20920/1‑116 (MQ=60)

                                                TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGATTTGCCAG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCAAAGAATGATTGTCATT                                                                                             >  LH00941:114:22FVMWLT1:2:1155:14636:9118/1‑151 (MQ=60)

                                                TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGTCCGCAAAACAGGAGCCACGGTGCATGGCACCGGCTTTTCCGG‑TGTTT‑‑T‑‑C‑‑GGGCA‑C‑GCCCACTCCCACCGACGGATTGCCCTT                                                                                             >  LH00941:114:22FVMWLT1:2:2246:35881:28686/1‑151 (MQ=60)

                                                            cccaccgtTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGTCCTCGAAACAGGCGCCCCGGTGCCTGGCCCCGGCTTTTCCGG‑TG‑TTcgcgggtccgcccgctcccaccaaattattggccttcaacgggcccat                                                                                        >  LH00941:114:22FVMWLT1:2:2261:46106:16869/9‑103 (MQ=60)

                                                            cccaccgtTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAGGCGCCGCGGTGCATGGTACCGGCTTTGCCGG‑TG‑TTCGT‑‑G‑‑GGGAA‑G‑C‑CCGCTCCCACAGACCGATTGCCATCAAAAGGGCCTAT                                                                                 >  LH00941:114:22FVMWLT1:2:2207:2202:3178/9‑151 (MQ=52)

                                                                    TTGGCACCAAAGAAGGATTGAGGTTGCAAAATGGTGGGAGTGGGCGTGCCCGCAAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGG‑T‑‑TT‑‑TCGC‑‑GGGCA‑CTC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAG                                                                         <  LH00941:114:22FVMWLT1:2:2123:44747:16532/151‑1 (MQ=45)

                                                                          CCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAGGAGCCGCGGTGCATGGCACCGGCTTTGCCGG‑TGTTT‑‑T‑‑C‑‑GGGCA‑C‑GCCCGCTCCCCCAAACCGATTGCCATTCAGCGGGACCATCGCTCCAGTGAAGG                                                                   >  LH00941:114:22FVMWLT1:2:2109:19277:3418/1‑151 (MQ=50)

                                                                          CCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCGCGGAACAAGCGCCACGGTGCATGGCCCCGGGTTTGCCGG‑TG‑TTcgcaggccccaccaatccccaaaaacgattgccattaagcgggcctcttgctccaatgaaag                                                                          >  LH00941:114:22FVMWLT1:2:2266:32387:24139/1‑89 (MQ=60)

                                                                          CCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGCCCGCGAAACAGGCGCCGCGGTGCCTGGCACCGGCTTTTCCGG‑TG‑TT‑‑Tgcgggcccgcccgctcccacaaactcagtgccattcaacagggcccccgcctccattacgg                                                                        >  LH00941:114:22FVMWLT1:2:2221:37157:1592/1‑90 (MQ=60)

                                                                          CCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGGAACAGGTGCCGCAGTGCATGGCCccggggttgtcggtttttgtgggggcgcccactcccaccgactgattgccactcagagggcctctcgcttcagtggaga                                                                            >  LH00941:114:22FVMWLT1:2:2120:11817:23098/1‑72 (MQ=60)

                                                                             CAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCTCGAAACAGGCGTCGCGGTGCATGGCACCAGCTTTTCCGGTTT‑TT‑‑T‑‑C‑‑GGGCA‑CAC‑CCGCTCCCAAAGAATGATTGCCATTCAGCGGGCCTctctctccaatgaaggccc                                                                >  LH00941:114:22FVMWLT1:2:1228:15154:19463/1‑132 (MQ=43)

                                                                             CAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAAGCGCCGCGGTGCATGGCACCAGCTTTTCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCCCAGACCGAGTGCCATTCAGCAGGCCCCTCTCTACAGTGACGGCCC                                                                >  LH00941:114:22FVMWLT1:2:2259:20747:17013/1‑151 (MQ=35)

                                                                                  GGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCtgtctcttatacacatctccgagcccacgagactcgtgcatccatctggttggtgagttttttgtcttgaaagagggggggtggggg                                                                    >  LH00941:114:22FVMWLT1:2:1167:2720:23578/1‑64 (MQ=60)

                                                                                  GGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCATGGCCCCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑CAC‑CCGCTCCCCCAGAATGATTGCCATTCAGCGGGCCTATCGCTACCGTGACGGACCGTTTT                                                           >  LH00941:114:22FVMWLT1:2:1163:26784:22217/1‑151 (MQ=52)

                                                                                                      gggggGCGGGCTTGCCCGCGAAACAGGCGCCGTGGTGCATGGCACTGTCTTTGCTGG‑TG‑TTCGT‑‑‑‑‑GGGCACC‑C‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAT                                       <  LH00941:114:22FVMWLT1:2:2165:29493:17813/146‑1 (MQ=60)

                                                                                                        GGAGCGGGTGTGCCCGCGAAACGTTTGCCGCGGTGCAGGGCACCGGTTTTGCCGT‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCA                                     <  LH00941:114:22FVMWLT1:2:2145:25055:4715/151‑1 (MQ=60)

                                                                                                                      tgtgtataagagacaGCGGTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAA                       <  LH00941:114:22FVMWLT1:2:1107:51034:17878/136‑1 (MQ=60)

                                                                                                                      CGCGAAACGGGCGCCGCGGTGCATGGCATTGGTTTTGCTGG‑TG‑‑T‑‑T‑‑CGAGGACA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAA                       <  LH00941:114:22FVMWLT1:2:1128:32054:11616/151‑1 (MQ=60)

                                                                                                                      AGCGAAACAGGCGCCGTGGTGCATGGCATCGGTTTTGCTGG‑TG‑TT‑‑TG‑C‑‑GGGCACC‑C‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAA                       <  LH00941:114:22FVMWLT1:2:2145:51663:19767/151‑1 (MQ=60)

                                                                                                                              ggcaaagcCGGTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCA               >  LH00941:114:22FVMWLT1:2:1211:36297:27869/9‑151 (MQ=60)

                                                                                                                                GCGCCGCGGTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG             >  LH00941:114:22FVMWLT1:2:2121:45579:4171/1‑151 (MQ=60)

                                                                                                                                   CCGCGGTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTACCGAATCATGACTCCCTTAGAACGCTATCAAGCAG          >  LH00941:114:22FVMWLT1:2:2202:31361:7053/1‑151 (MQ=60)

                                                                                                                                   CCGCGGTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAG          >  LH00941:114:22FVMWLT1:2:2162:18926:26637/1‑151 (MQ=60)

                                                                                                                                   CCGCGGTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAG          >  LH00941:114:22FVMWLT1:2:1269:37435:26540/1‑151 (MQ=60)

                                                                                                                                   CCGCGGTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAG          >  LH00941:114:22FVMWLT1:2:2202:31379:7053/1‑151 (MQ=60)

                                                                                                                                     GCGGTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAT        >  LH00941:114:22FVMWLT1:2:1255:4060:18342/1‑151 (MQ=60)

                                                                                                                                     GCGGTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAT        >  LH00941:114:22FVMWLT1:2:2243:27857:10335/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGANTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2260:42418:24763/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTCTCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1240:52125:11857/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2223:21330:26797/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2202:5253:20151/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2204:20738:4827/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2215:6760:16516/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2225:43730:25532/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2223:21311:26797/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2140:18704:5916/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1106:47983:15796/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1107:24686:3338/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1119:19481:25548/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2265:11604:5019/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1133:36094:2057/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1141:38572:10480/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1141:38590:10480/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1141:47733:7517/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1141:7037:24619/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1141:7055:24619/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1167:24667:4491/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1213:14045:6620/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1223:12020:10864/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1223:12029:10848/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1241:3690:24203/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2109:44618:27837/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2116:4328:22521/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1105:12140:11905/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCATTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1167:24686:4491/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAATCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:1239:25028:21160/1‑151 (MQ=60)

                                                                                                                                        GTGCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCACGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTG     >  LH00941:114:22FVMWLT1:2:2116:3635:2329/1‑151 (MQ=60)

                                                                                                                                          GCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:2255:48880:2873/1‑151 (MQ=60)

                                                                                                                                          GCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:2117:26784:12417/1‑151 (MQ=60)

                                                                                                                                          GCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:2223:12935:8158/1‑151 (MQ=60)

                                                                                                                                          GCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:2209:25028:23242/1‑151 (MQ=60)

                                                                                                                                          GCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:2147:50119:8542/1‑151 (MQ=60)

                                                                                                                                          GCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:2143:37194:12161/1‑151 (MQ=60)

                                                                                                                                          GCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:2140:48686:13714/1‑151 (MQ=60)

                                                                                                                                          GCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:2122:51478:19607/1‑151 (MQ=60)

                                                                                                                                          GCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:1216:35317:25756/1‑151 (MQ=60)

                                                                                                                                          GCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:1128:5715:24379/1‑151 (MQ=60)

                                                                                                                                          GCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA   >  LH00941:114:22FVMWLT1:2:1116:31878:20504/1‑151 (MQ=60)

                                                                                                                                          GCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACG‑ATCCTGCCTCCCTTAGAACGCTATCAAGCAGATCTGAAA  >  LH00941:114:22FVMWLT1:2:2127:31499:10143/1‑151 (MQ=60)

                                                                                                                                          GCATGGCACCGGCTTTGCCGG‑TG‑TTC‑G‑‑C‑‑GGGCA‑C‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACG‑ATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAA  >  LH00941:114:22FVMWLT1:2:2270:35216:26156/1‑151 (MQ=60)

TTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGG‑TG‑TT‑‑T‑‑C‑‑GGGCA‑C‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAA  >  AE015451_phaM1/1499335‑1499622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`