Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F42 I4 R1 76 794.2 35354760 99.3% 35107276 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 5,681,415 +CGGG intergenic (‑133/+71) PP_4986 CDS (Channel protein_ hemolysin III family) ← / ← PP_4987 CDS (putative Chemotaxis protein) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,681,4151.C100.0% 312.2 / NA 86intergenic (‑133/+71)PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (71/15);  total (71/15)
*AE015451_phaM15,681,4152.G98.8% 312.9 / NA 86intergenic (‑133/+71)PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (69/15);  minor base A (1/0);  total (71/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*AE015451_phaM15,681,4153.G97.7% 309.4 / ‑4.4 86intergenic (‑133/+71)PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (69/15);  minor base A (2/0);  total (71/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*AE015451_phaM15,681,4154.G100.0% 318.6 / NA 86intergenic (‑133/+71)PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein)–/–
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (71/15);  total (71/15)

Only 100 of 102 total aligned reads displayed.

ACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTC  >  AE015451_phaM1/5681279‑5681530
                                                                                                                                           ||||                                                                                                                   
aCATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  2:10081155/137‑1 (MQ=255)
aCATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  2:5434764/137‑1 (MQ=255)
aCATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  1:6340213/137‑1 (MQ=255)
aCATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  1:8928243/137‑1 (MQ=255)
aCATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  2:10081126/137‑1 (MQ=255)
aCATACAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  1:2480324/137‑1 (MQ=255)
    gCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccggg                                                                                                                     <  1:6919/137‑5 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGGTGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  2:6229929/137‑6 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  1:4215292/137‑6 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  2:7363467/137‑6 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  2:6229891/137‑6 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  2:7571098/137‑6 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  2:1852175/137‑6 (MQ=255)
     cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgccgggg                                                                                                                    <  2:1851799/137‑6 (MQ=255)
          acaGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         <  1:16856419/127‑1 (MQ=255)
          acaGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                         >  2:16856419/1‑127 (MQ=255)
                   cttaGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGTGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGccc                                                                                                      <  2:8722025/134‑1 (MQ=37)
                    tGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCt                                                                                                     >  2:5252850/1‑137 (MQ=37)
                     gagttGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  2:13411725/133‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTTTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:2714552/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  2:14556221/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:5974397/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  2:14964073/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:2409052/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:238946/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  2:12296190/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  2:6781079/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  2:723655/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:12095756/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:11822561/137‑1 (MQ=37)
                     gAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTAATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:5128752/137‑1 (MQ=37)
                                ggTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGacac                                                                                           >  2:2460001/1‑137 (MQ=37)
                                ggTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGAAT‑‑TTTGTCGTGGAAGGGGGGCGAAGCGGCCCCAGGTTTTCATTTTTACCACATGAAACGCCAGGGCTGCTGCGCGGCCCTCCCGCGACACaa                                                                                         >  2:11723773/1‑137 (MQ=255)
                                        ttgtGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGTCT‑‑TGTTTCGCTAAAGGGGAGCGGAGCAGCCCCCGGCTTTTCCTGGCACTGCACGAACCGCCTAGGCTGCTGTGTAGACCCCTCGCGACACAAGGCTGccc                                                                                 >  2:16739792/1‑135 (MQ=255)
                                                      gCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTNCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    <  1:17016609/104‑1 (MQ=21)
                                                      gCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTa                                                                                                    >  2:17016609/1‑104 (MQ=25)
                                                                                           agggggCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGCCAgg                                >  2:11180528/3‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCCGGCTTTCATTGCCCCTGCATGAACCGCCGGGGCTGCTTCGCAGCCATATCGCGACACAAGGCCGCCCCCACTCCAGACCACCAGGCCCTGCGCATCCCATCCTCACCAAGGTCAgg                                >  1:6216686/1‑137 (MQ=25)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTCTCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTACCCAAGGTCAgg                                >  2:16643336/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCCGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:2030152/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCGAGGTCggg                                >  2:13748265/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCACGGTCAgg                                >  1:10683428/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:15575164/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:16542238/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:13765638/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:16708577/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:4731243/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:5762194/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:6514954/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:7153167/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:9485854/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:13964664/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:8872527/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:8195999/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:16721276/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:16877790/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:16877791/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:17156932/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:1969386/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  2:11258741/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:3196605/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:3196650/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCACCTCACATCCTAACCAAGGTCAgg                                >  1:11033762/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAAACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:7580492/1‑137 (MQ=38)
                                                                                           aaGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTANCGCGACACAAGGCCGCTCCCCCTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                >  1:15195180/1‑137 (MQ=38)
                                                                                             ggggCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGtt                              >  1:244510/1‑137 (MQ=38)
                                                                                             ggggCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGtt                              >  1:12886663/1‑137 (MQ=38)
                                                                                                  gcgAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGa                         >  2:4205431/1‑137 (MQ=38)
                                                                                                  gcgAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGa                         >  1:14039546/1‑137 (MQ=38)
                                                                                                    gAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATc                       >  1:6437302/1‑137 (MQ=38)
                                                                                                    gAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATc                       >  2:6520685/1‑137 (MQ=38)
                                                                                                    gAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATc                       >  1:2292117/1‑137 (MQ=38)
                                                                                                        cGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCcgcg                   >  1:16115150/1‑137 (MQ=38)
                                                                                                             ccAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACg              >  1:9077445/1‑137 (MQ=38)
                                                                                                             ccAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACg              >  1:298202/1‑137 (MQ=38)
                                                                                                             ccAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACg              >  1:298236/1‑137 (MQ=38)
                                                                                                                   tttCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAg        >  1:3295579/1‑137 (MQ=38)
                                                                                                                       aTTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGNCCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCtt    >  1:15219483/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACCCCAGGCCGCTCCCCCTCCAAACCAACCGGCCCTGCGCACCACACCCTACCCAAGGTCAGGCTGCAAATCCGCGTCACGCACCAGCCtgg   >  2:9482473/1‑135 (MQ=19)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  1:11197343/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:11731685/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:12049258/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:1349395/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  1:11197342/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:5392493/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:1349396/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:1349436/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  1:5623363/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:2881354/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:2881353/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:14453516/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:1720331/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:15372936/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACNCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  1:16260305/1‑137 (MQ=38)
                                                                                                                        ttGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCACTATCGCGACACAAGGCCACTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCCCGCACCAGCCttt   >  2:6868227/1‑137 (MQ=255)
                                                                                                                        ttGCCACTGCATGAACCGCCGAGGCTACTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCAATCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCCAGGTCCGGTTGCAGCTCCACGTCACACACCAGCCttt   >  2:5672237/1‑137 (MQ=37)
                                                                                                                        ttGCCACTGCATAAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCCGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCttt   >  2:14453481/1‑137 (MQ=37)
                                                                                                                         tGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTc  >  1:6398990/1‑137 (MQ=38)
                                                                                                                         tGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTc  >  1:6398955/1‑137 (MQ=38)
                                                                                                                         tGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTc  >  1:5069640/1‑137 (MQ=38)
                                                                                                                                           ||||                                                                                                                   
ACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCT‑‑TGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTC  >  AE015451_phaM1/5681279‑5681530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGC  >  AE015451_phaM1/5681270‑5681564
                                                                                                                                                 |                                                                                                                                                         
CACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcgggg                                                                                                                                                      <  LH00941:114:22FVMWLT1:2:1159:24242:28318/151‑6 (MQ=60)
CACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcgggg                                                                                                                                                      <  LH00941:114:22FVMWLT1:2:1117:19989:29535/151‑6 (MQ=60)
 ACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggc                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1207:51321:27181/151‑7 (MQ=60)
 ACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggc                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:2103:28513:19639/151‑7 (MQ=60)
  CCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCACGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggct                                                                                                                                                    <  LH00941:114:22FVMWLT1:2:2108:31490:13106/151‑8 (MQ=60)
  CCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGACCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggct                                                                                                                                                    >  LH00941:114:22FVMWLT1:2:1204:8988:17205/1‑144 (MQ=60)
    GTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgc                                                                                                                                                  <  LH00941:114:22FVMWLT1:2:2220:5299:1945/151‑10 (MQ=60)
      AATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTG                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1222:46781:23770/151‑1 (MQ=60)
         ACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGC                                                                                                                                             <  LH00941:114:22FVMWLT1:2:2102:44507:10960/151‑1 (MQ=60)
         ACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGC                                                                                                                                             <  LH00941:114:22FVMWLT1:2:2258:50109:13554/151‑1 (MQ=60)
         ACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGC                                                                                                                                             <  LH00941:114:22FVMWLT1:2:2134:22994:13666/151‑1 (MQ=60)
         ACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGC                                                                                                                                             <  LH00941:114:22FVMWLT1:2:1232:36048:1464/151‑1 (MQ=60)
         ACATACAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGC                                                                                                                                             <  LH00941:114:22FVMWLT1:2:1140:7555:27693/151‑1 (MQ=60)
            TGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGC                                                                                                                                          >  LH00941:114:22FVMWLT1:2:2233:28310:24507/1‑151 (MQ=60)
             GCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCC                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1101:30926:4411/151‑1 (MQ=60)
              CAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCC                                                                                                                                        <  LH00941:114:22FVMWLT1:2:1168:42029:3434/151‑1 (MQ=60)
                  CACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATC                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1253:49573:19415/151‑1 (MQ=60)
                   ACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                   <  LH00941:114:22FVMWLT1:2:1102:35280:4523/151‑1 (MQ=60)
                   ACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                   <  LH00941:114:22FVMWLT1:2:2158:12510:28782/151‑1 (MQ=60)
                   ACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                   <  LH00941:114:22FVMWLT1:2:2204:39653:1657/151‑1 (MQ=60)
                   ACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                   <  LH00941:114:22FVMWLT1:2:2204:39663:1641/151‑1 (MQ=60)
                   ACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                   <  LH00941:114:22FVMWLT1:2:1143:21921:14147/151‑1 (MQ=60)
                   ACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                   <  LH00941:114:22FVMWLT1:2:2208:46966:27229/151‑1 (MQ=60)
                   ACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCG                                                                                                                                   <  LH00941:114:22FVMWLT1:2:1114:12972:5948/151‑1 (MQ=60)
                     ATACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCG                                                                                                                                 <  LH00941:114:22FVMWLT1:2:2223:13139:9086/151‑1 (MQ=60)
                        CCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACA                                                                                                                              <  LH00941:114:22FVMWLT1:2:2155:22042:24187/151‑1 (MQ=60)
                              agatgtgtataagagacaGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTNCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                        <  LH00941:114:22FVMWLT1:2:2260:44840:27005/133‑1 (MQ=60)
                              GAGGTGGGCTGGGTTCCTGTTTTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                        <  LH00941:114:22FVMWLT1:2:1144:31647:18502/151‑1 (MQ=60)
                              GAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                        <  LH00941:114:22FVMWLT1:2:1226:15321:7517/151‑1 (MQ=60)
                              GAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                        <  LH00941:114:22FVMWLT1:2:1104:52023:27501/151‑1 (MQ=60)
                              GAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                        <  LH00941:114:22FVMWLT1:2:1139:40606:23834/151‑1 (MQ=60)
                              GAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                        <  LH00941:114:22FVMWLT1:2:2149:11327:3994/151‑1 (MQ=60)
                              GAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                        <  LH00941:114:22FVMWLT1:2:2153:31185:13602/151‑1 (MQ=60)
                              GAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTAATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGC                                                                                                                        <  LH00941:114:22FVMWLT1:2:1212:49592:22521/151‑1 (MQ=60)
                                         GGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCtgtctcttatacacatctccg                                                                                                             >  LH00941:114:22FVMWLT1:2:2152:20257:15203/1‑130 (MQ=60)
                                                                     cacaccagatgtcttctaatgtctcgtgggctcggagatgtgtataagagacagTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATC                                                                                 <  LH00941:114:22FVMWLT1:2:1131:30630:26028/97‑1 (MQ=60)
                                                                            agagacaggctTTGTGTCGCGAAGGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCAGGAACCGCTGGGGTTGTTGCGCAGCCCTATCGCGACAAAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCA                                                                          <  LH00941:114:22FVMWLT1:2:2129:39875:28974/140‑1 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCCGGCTTTCATTGCCCCTGCATGAACCGCCGGGGCTGCTTCGCAGCCATATCGCGACACAAGGCCGCCCCCACTCCAGACCACCAGGCCCTGCGCATCCCATCCTCACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:1230:51709:2713/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCCGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:1133:36020:23066/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCACGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:2130:6612:26156/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:2212:37028:28078/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:1260:14988:29615/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:2263:7481:2617/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:2101:44183:13186/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:2258:42085:23002/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:2258:42066:23002/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:2256:47280:11248/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:1152:23170:6829/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:1132:37869:24058/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:1152:23179:6813/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCACCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:2136:49601:16484/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAAACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:1251:3949:14563/1‑151 (MQ=60)
                                                                                    GCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTANCGCGACACAAGGCCGCTCCCCCTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                                  >  LH00941:114:22FVMWLT1:2:2232:30898:14867/1‑151 (MQ=60)
                                                                                      CTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTT                                                                >  LH00941:114:22FVMWLT1:2:1105:41114:1464/1‑151 (MQ=60)
                                                                                      CTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTT                                                                >  LH00941:114:22FVMWLT1:2:2165:5345:22553/1‑151 (MQ=60)
                                                                                           GTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGA                                                           >  LH00941:114:22FVMWLT1:2:2214:15940:4971/1‑151 (MQ=60)
                                                                                             CGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATC                                                         >  LH00941:114:22FVMWLT1:2:1233:6455:16917/1‑151 (MQ=60)
                                                                                             CGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATC                                                         >  LH00941:114:22FVMWLT1:2:1137:51653:27950/1‑151 (MQ=60)
                                                                                                 AAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCG                                                     >  LH00941:114:22FVMWLT1:2:2246:33764:27998/1‑151 (MQ=60)
                                                                                                   AGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggcctgtctcttatacacatctccgagccaacgagacattagaagacatctggggtgtggtttttttttttgaaaaagggggggggggggggggggggggg                                                   >  LH00941:114:22FVMWLT1:2:1150:22763:10960/1‑47 (MQ=60)
                                                                                                      GGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACG                                                >  LH00941:114:22FVMWLT1:2:1105:14720:26717/1‑151 (MQ=60)
                                                                                                      GGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACG                                                >  LH00941:114:22FVMWLT1:2:1105:14710:26701/1‑151 (MQ=60)
                                                                                                      GGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACG                                                >  LH00941:114:22FVMWLT1:2:2104:40911:24139/1‑151 (MQ=60)
                                                                                                            AAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAG                                          >  LH00941:114:22FVMWLT1:2:1153:23900:22922/1‑151 (MQ=60)
                                                                                                                GGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGNCCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTT                                      >  LH00941:114:22FVMWLT1:2:2232:19693:25724/1‑151 (MQ=60)
                                                                                                                 GCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTT                                     >  LH00941:114:22FVMWLT1:2:1220:50267:19719/1‑151 (MQ=60)
                                                                                                                 GCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTT                                     >  LH00941:114:22FVMWLT1:2:2139:44017:6012/1‑151 (MQ=60)
                                                                                                                 GCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTT                                     >  LH00941:114:22FVMWLT1:2:2139:43998:6012/1‑151 (MQ=60)
                                                                                                                 GCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACNCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTT                                     >  LH00941:114:22FVMWLT1:2:2249:41539:6492/1‑151 (MQ=60)
                                                                                                                  CCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTC                                    >  LH00941:114:22FVMWLT1:2:1211:48538:23994/1‑151 (MQ=60)
                                                                                                                  CCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTC                                    >  LH00941:114:22FVMWLT1:2:1232:30954:28094/1‑151 (MQ=60)
                                                                                                                  CCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTC                                    >  LH00941:114:22FVMWLT1:2:1232:30945:28078/1‑151 (MQ=60)
                                                                                                                      AGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAAT                                >  LH00941:114:22FVMWLT1:2:1114:26322:22729/1‑151 (MQ=60)
                                                                                                                       GGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCCGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATG                               >  LH00941:114:22FVMWLT1:2:1243:12556:7469/1‑151 (MQ=60)
                                                                                                                       GGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCctgtctcttatacaca                               >  LH00941:114:22FVMWLT1:2:1203:43721:16036/1‑135 (MQ=60)
                                                                                                                       GGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATG                               >  LH00941:114:22FVMWLT1:2:2265:48917:8446/1‑151 (MQ=60)
                                                                                                                       GGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATG                               >  LH00941:114:22FVMWLT1:2:1224:41715:8206/1‑151 (MQ=60)
                                                                                                                       GGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATG                               >  LH00941:114:22FVMWLT1:2:1215:37666:28990/1‑151 (MQ=60)
                                                                                                                       GGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATG                               >  LH00941:114:22FVMWLT1:2:1119:10227:10512/1‑151 (MQ=60)
                                                                                                                       GGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATG                               >  LH00941:114:22FVMWLT1:2:2165:45626:23978/1‑151 (MQ=60)
                                                                                                                       GGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATG                               >  LH00941:114:22FVMWLT1:2:1116:3145:14739/1‑151 (MQ=60)
                                                                                                                       GGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATG                               >  LH00941:114:22FVMWLT1:2:2160:39413:22986/1‑151 (MQ=60)
                                                                                                                       GGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATG                               >  LH00941:114:22FVMWLT1:2:2160:39404:22970/1‑151 (MQ=60)
                                                                                                                        GCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGC                              >  LH00941:114:22FVMWLT1:2:2155:15450:24555/1‑151 (MQ=60)
                                                                                                                           TTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACGCAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAA                           >  LH00941:114:22FVMWLT1:2:1158:41826:1865/1‑151 (MQ=60)
                                                                                                                           TTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCctgtctct                           >  LH00941:114:22FVMWLT1:2:2237:36214:5916/1‑143 (MQ=60)
                                                                                                                           TTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAC                           >  LH00941:114:22FVMWLT1:2:1141:49277:2089/1‑151 (MQ=60)
                                                                                                                           TTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAC                           >  LH00941:114:22FVMWLT1:2:2214:24140:25932/1‑151 (MQ=60)
                                                                                                                           TTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAC                           >  LH00941:114:22FVMWLT1:2:2228:18066:18934/1‑151 (MQ=60)
                                                                                                                           TTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAC                           >  LH00941:114:22FVMWLT1:2:2270:32322:6316/1‑151 (MQ=60)
                                                                                                                           TTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAC                           >  LH00941:114:22FVMWLT1:2:2158:3958:5644/1‑151 (MQ=60)
                                                                                                                           TTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAC                           >  LH00941:114:22FVMWLT1:2:1158:41844:1833/1‑151 (MQ=60)
                                                                                                                           TTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAC                           >  LH00941:114:22FVMWLT1:2:1221:44692:10287/1‑151 (MQ=60)
                                                                                                                           TTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAC                           >  LH00941:114:22FVMWLT1:2:2121:43139:17461/1‑151 (MQ=60)
                                                                                                                           TTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAC                           >  LH00941:114:22FVMWLT1:2:2110:20026:27005/1‑151 (MQ=60)
                                                                                                                           TTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAC                           >  LH00941:114:22FVMWLT1:2:2110:20017:27021/1‑151 (MQ=60)
                                                                                                                             CATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCA                         >  LH00941:114:22FVMWLT1:2:1206:42085:17589/1‑151 (MQ=60)
                                                                                                                                 GCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTC                     >  LH00941:114:22FVMWLT1:2:2156:46282:29151/1‑151 (MQ=60)
                                                                                                                                 GCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTC                     >  LH00941:114:22FVMWLT1:2:1247:26868:6669/1‑151 (MQ=60)
                                                                                                                                  CCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCC                    >  LH00941:114:22FVMWLT1:2:2262:24445:21785/1‑151 (MQ=60)
                                                                                                                                       GCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTAC               >  LH00941:114:22FVMWLT1:2:1148:46264:27517/1‑151 (MQ=60)
                                                                                                                                       GCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTAC               >  LH00941:114:22FVMWLT1:2:2268:48140:24106/1‑151 (MQ=60)
                                                                                                                                       GCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTAC               >  LH00941:114:22FVMWLT1:2:2268:48131:24122/1‑151 (MQ=60)
                                                                                                                                       GCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTAC               >  LH00941:114:22FVMWLT1:2:1227:49019:2697/1‑151 (MQ=60)
                                                                                                                                       GCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTAC               >  LH00941:114:22FVMWLT1:2:2143:4513:14355/1‑151 (MQ=60)
                                                                                                                                       GCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTAC               >  LH00941:114:22FVMWLT1:2:2225:25777:21785/1‑151 (MQ=60)
                                                                                                                                       GCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTAC               >  LH00941:114:22FVMWLT1:2:1135:22985:15187/1‑151 (MQ=60)
                                                                                                                                       GCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATACGACCAGGTCCGGTAC               >  LH00941:114:22FVMWLT1:2:1148:46273:27501/1‑151 (MQ=60)
                                                                                                                                                GG‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCtgtctcttatac  >  LH00941:114:22FVMWLT1:2:1224:51681:24731/1‑139 (MQ=60)
                                                                                                                                                GG‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGC  >  LH00941:114:22FVMWLT1:2:1217:48307:11424/1‑151 (MQ=60)
                                                                                                                                                GG‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGC  >  LH00941:114:22FVMWLT1:2:1220:43823:3402/1‑151 (MQ=60)
                                                                                                                                                GG‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGC  >  LH00941:114:22FVMWLT1:2:2101:49786:22954/1‑151 (MQ=60)
                                                                                                                                                GG‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGC  >  LH00941:114:22FVMWLT1:2:1220:45062:2986/1‑151 (MQ=60)
                                                                                                                                                GG‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGC  >  LH00941:114:22FVMWLT1:2:2142:33099:17749/1‑151 (MQ=60)
                                                                                                                                                GG‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGC  >  LH00941:114:22FVMWLT1:2:2151:45348:9663/1‑151 (MQ=60)
                                                                                                                                                GG‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAACCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGC  >  LH00941:114:22FVMWLT1:2:1220:43832:3418/1‑151 (MQ=60)
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CACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGC  >  AE015451_phaM1/5681270‑5681564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑