Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F84 I10 R1 267 426.1 13521957 97.0% 13116298 150.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,271,130 C→T E914E (GAG→GAA)  uvrA ← ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,271,1300CT97.4% 1146.4 / 12.1 343E914E (GAG→GAA) uvrAATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC
Reads supporting (aligned to +/- strand):  ref base C (3/6);  new base T (169/165);  total (172/171)
Fisher's exact test for biased strand distribution p-value = 3.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01

Only 100 of 349 total aligned reads displayed.

GAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCG  >  NC_000913/4270982‑4271277
                                                                                                                                                    |                                                                                                                                                   
gaagaaAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGAtttc                                                                                                                                                   <  4:845469/151‑1 (MQ=255)
 aagaaAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTcg                                                                                                                                                  >  5:694790/1‑151 (MQ=255)
  agaaAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAACGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTGTCTGGCGTACCGGAGACGAGGAGTTcgc                                                                                                                                                 >  3:1174211/1‑151 (MQ=255)
      aaaCGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCgccgcc                                                                                                                                             <  5:1311372/151‑1 (MQ=255)
      aaaCGTAAATTGCTGGTGCAACTATGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGACGTGTGTGATGCTTCGCACTCCGCGACGGTTTATGGCGTACCGGAGACGAGGATTTCgccgcc                                                                                                                                             >  6:948351/1‑151 (MQ=255)
       aaCGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGcca                                                                                                                                            <  1:60029/151‑1 (MQ=255)
         cGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGccacc                                                                                                                                          >  2:959135/1‑151 (MQ=255)
           tAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGCCGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCcaccac                                                                                                                                        >  4:633267/1‑151 (MQ=255)
           tAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCcaccac                                                                                                                                        >  8:1048745/1‑151 (MQ=255)
            aaaTTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACt                                                                                                                                       >  7:1114283/1‑151 (MQ=255)
            aaaTTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACt                                                                                                                                       >  7:295913/1‑151 (MQ=255)
            aaaTTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACt                                                                                                                                       <  8:1645783/151‑1 (MQ=255)
             aaTTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTg                                                                                                                                      >  2:337276/1‑151 (MQ=255)
             aaTTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTg                                                                                                                                      <  5:1152899/151‑1 (MQ=255)
                 gCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTgccgc                                                                                                                                  >  7:1581283/1‑151 (MQ=255)
                   tGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCt                                                                                                                                >  5:1587786/1‑151 (MQ=255)
                           ctctGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCCCTCCGCGACGGTTTCTGGCGTACCGGAGCCGAGGATTTCGCCGCCCCCACTGCCGCCTTCTGGTcc                                                                                                                        >  5:33228/1‑151 (MQ=255)
                              tGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTccc                                                                                                                       >  4:1248286/1‑149 (MQ=255)
                              tGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTccc                                                                                                                       >  8:838077/1‑149 (MQ=255)
                               gAAAGGAAAAGGCCGCTCAGAACGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGCCGGTTTCTGGCGTACCGGAGCCGAGGATTTCGCCGGCACCACTGCCGCCTTCTGGTCCCAgg                                                                                                                    >  3:834582/1‑151 (MQ=255)
                               gAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTccc                                                                                                                       <  5:846680/148‑1 (MQ=255)
                               gAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTccc                                                                                                                       <  3:1253324/148‑1 (MQ=255)
                               gAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAgg                                                                                                                    <  8:1563783/151‑1 (MQ=255)
                               gAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAgg                                                                                                                    >  5:813481/1‑151 (MQ=255)
                               gAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAgg                                                                                                                    >  5:77269/1‑151 (MQ=255)
                                 aaGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTc                                                                                                                  <  2:1036209/151‑1 (MQ=255)
                                   ggAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCg                                                                                                                 >  3:739792/1‑150 (MQ=255)
                                     aaaaGGCCGCTCAGAAAGTGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGAc                                                                                                               >  2:1499289/1‑150 (MQ=255)
                                       aaGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAAt                                                                                                            <  2:655101/151‑1 (MQ=255)
                                       aaGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAAt                                                                                                            <  8:1210323/151‑1 (MQ=255)
                                       aaGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAAt                                                                                                            >  6:221540/1‑151 (MQ=255)
                                          gCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTCTGTGATGCTTCGCCCTCCGCGACGGTTTCTGCCGTACCGGAGACCAGGATTTCGCCGCCCCCACTGCCGCCTTCTGGTCCCAGGTCGACAATcc                                                                                                          >  2:1468461/1‑150 (MQ=255)
                                               tCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTc                                                                                                      >  4:721773/1‑149 (MQ=255)
                                                cAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCa                                                                                                     <  3:1399407/149‑1 (MQ=255)
                                                   aaaGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGt                                                                                                >  8:1126527/1‑151 (MQ=255)
                                                    aaGAGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTTCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGtt                                                                                               <  7:1651105/151‑1 (MQ=255)
                                                     agcgtagttAACGATTACAGCATCGGCTTTAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGttt                                                                                              <  8:439652/144‑1 (MQ=255)
                                                      gCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGtttt                                                                                             >  7:913205/1‑151 (MQ=255)
                                                       cGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTg                                                                                            >  3:1670423/1‑151 (MQ=255)
                                                        ggCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTg                                                                                            >  4:1055316/1‑150 (MQ=255)
                                                         gCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGttttca                                                                                           >  6:486172/1‑148 (MQ=255)
                                                            ttAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCAc                                                                                       >  1:467560/1‑151 (MQ=255)
                                                            ttAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTCATCAc                                                                                       >  4:1667667/1‑151 (MQ=255)
                                                             tAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACg                                                                                      >  5:418588/1‑151 (MQ=255)
                                                               aCGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTc                                                                                    <  4:1575494/151‑1 (MQ=255)
                                                                cGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCg                                                                                   <  4:384066/151‑1 (MQ=255)
                                                                  aTTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCgag                                                                                 <  7:41486/151‑1 (MQ=255)
                                                                    tACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCgaga                                                                                <  4:22343/150‑1 (MQ=255)
                                                                     aCAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGAtt                                                                              <  7:877102/151‑1 (MQ=255)
                                                                       aGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATtgt                                                                            <  2:100572/151‑1 (MQ=255)
                                                                       aGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGCTCGACAATCCAGACAGCGGAATTGATCACCTCGAGAttct                                                                            >  6:1431307/1‑149 (MQ=255)
                                                                         cATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGc                                                                          >  6:1391005/1‑151 (MQ=255)
                                                                            cGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTc                                                                        >  6:361659/1‑150 (MQ=255)
                                                                            cGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTc                                                                        <  6:1041785/150‑1 (MQ=255)
                                                                            cGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTc                                                                        <  6:1203787/150‑1 (MQ=255)
                                                                            cGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCa                                                                       <  6:1636169/151‑1 (MQ=255)
                                                                              gcgtAAGGAAGCGTTCCGTGTGTGATGCGTCGCACGCCGGGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCCTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAAt                                                                     <  8:670935/148‑1 (MQ=255)
                                                                              gCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAAt                                                                     >  5:988309/1‑151 (MQ=255)
                                                                               cTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATc                                                                    >  3:392184/1‑151 (MQ=255)
                                                                                  aaGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGAGGGTTTGTGCCGTACGGGAGCCGAGGATTTCGCCGCCACCCCTGCCGCCTGGTGGTCCCAGGGCGACAAGCCAGTCAGCGGTTTTGATCACGTCGCGATTTTCCTCAATaacc                                                                 >  7:1276979/1‑151 (MQ=255)
                                                                                  aaGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATcacc                                                                 >  2:1120078/1‑151 (MQ=255)
                                                                                  aaGGAAGCGTGCCGTGTGTGATGCTTCGCACACCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGACGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATcacc                                                                 >  8:1271497/1‑151 (MQ=255)
                                                                                    ggAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATcaccac                                                               >  1:1336163/1‑151 (MQ=255)
                                                                                     gAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTGTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACcaca                                                              <  8:750965/151‑1 (MQ=255)
                                                                                     gAAGCGAGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACcaca                                                              <  1:1031726/151‑1 (MQ=255)
                                                                                      aaGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACcaca                                                              >  7:1429980/1‑150 (MQ=255)
                                                                                       aGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAAt                                                            >  3:547378/1‑151 (MQ=255)
                                                                                         cGTGCCGTGGGTGATGCTTCGCACTCCGCGACGGGTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATgg                                                          <  6:1676960/151‑1 (MQ=255)
                                                                                          gTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGgt                                                         <  4:585063/151‑1 (MQ=255)
                                                                                             ccGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGtt                                                      >  7:567936/1‑151 (MQ=255)
                                                                                             ccGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGtt                                                      <  6:646143/151‑1 (MQ=255)
                                                                                              cGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTg                                                     >  1:736549/1‑151 (MQ=255)
                                                                                                   gggATGCTTCGCACTCCGCGACGGGTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTg                                                <  7:1061409/149‑1 (MQ=255)
                                                                                                     gATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGAt                                              <  4:1462219/151‑1 (MQ=255)
                                                                                                     gATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGAt                                              <  4:136837/151‑1 (MQ=255)
                                                                                                      aTGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATc                                             <  5:105414/151‑1 (MQ=255)
                                                                                                      aTGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATc                                             >  5:489516/1‑151 (MQ=255)
                                                                                                           tCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCgcgc                                         >  2:863969/1‑150 (MQ=255)
                                                                                                              cACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCAAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGtt                                     >  8:1678623/1‑151 (MQ=255)
                                                                                                                   cgcgACGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGCTGTTGCCCTGATCGCGCAGTTTATGc                                >  1:1457856/1‑151 (MQ=255)
                                                                                                                       aCGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTa                            <  8:688967/151‑1 (MQ=255)
                                                                                                                        cGGTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTAc                           <  6:798412/151‑1 (MQ=255)
                                                                                                                         ggTTTCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACg                          >  3:1435060/1‑151 (MQ=255)
                                                                                                                            ttCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTc                        <  2:740044/150‑1 (MQ=255)
                                                                                                                            ttCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTc                        <  8:292156/150‑1 (MQ=255)
                                                                                                                             tCTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCg                       >  7:1358253/1‑150 (MQ=255)
                                                                                                                              cTGGCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCg                       >  1:358961/1‑149 (MQ=255)
                                                                                                                                ggCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCCGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGc                    <  6:71628/150‑1 (MQ=255)
                                                                                                                                ggCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGa                      <  3:335715/148‑1 (MQ=255)
                                                                                                                                ggCGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGa                      <  2:1269867/148‑1 (MQ=255)
                                                                                                                                  cGTACCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGt                 <  1:183338/151‑1 (MQ=255)
                                                                                                                                     aCCGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGc              <  4:118349/151‑1 (MQ=255)
                                                                                                                                      ccGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCt             <  1:837293/151‑1 (MQ=255)
                                                                                                                                       cGGAGACGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTg            >  7:495274/1‑151 (MQ=255)
                                                                                                                                          agaCGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAat         >  3:993642/1‑151 (MQ=255)
                                                                                                                                          agaCGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAat         >  6:151165/1‑151 (MQ=255)
                                                                                                                                           gaCGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAata        <  8:1478972/151‑1 (MQ=255)
                                                                                                                                           gaCGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCCGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCATGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAat         >  6:849900/1‑150 (MQ=255)
                                                                                                                                             cGAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATc      <  2:467041/151‑1 (MQ=255)
                                                                                                                                              gAGGATTTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCg     >  7:754933/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTGCCGCCTTCTGGTCCCAGGTCGACAATCCAGTCAGCGGTTTTGATCACGTCGAGATTGTGCTCAATCACCACAATGGTGTTGCCCTGATCGCGCAGTTTATGCAGTACGTCGAGCAGTTGCTGAATATCGGCG  >  NC_000913/4270982‑4271277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A