Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F90 I0 R1 54 129.4 2031886 96.4% 1958738 285.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,442,896 A→T 54.7% F401Y (TTC→TAC)  secY ← preprotein translocase membrane subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009133,442,8960A→T54.7% 177.9 0.0 ‑0.4127F401Y (TTC→TAC) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  new base (28/41):  ref base (22/33):  total (53/74)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.10e-01

Only 100 of 127 total aligned reads displayed.

AAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG  >  NC_000913/3442601‑3443184
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                
   aaTATGCGAAAAAATCAGCCTTTGCGCTGTTTATGCTTCGGCTCGGCACTGCAAAGCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAAc                                                                                                                                                                                                                                                                                          <  1:409761/301‑1 (MQ=255)
   aaTATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAAc                                                                                                                                                                                                                                                                                          <  2:409922/301‑1 (MQ=255)
           aaaaaaTCAGCGTTTGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACttt                                                                                                                                                                                                                                                                                  <  1:78047/301‑1 (MQ=255)
             aaaaTCAGCCTTGGCGCGTTTTATGCTTCGGCTAGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCa                                                                                                                                                                                                                                                                                <  1:302087/301‑1 (MQ=255)
              aaaTCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCCCTTGAGCCATAAAGTCCATAATCACGACAACAACGACAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCAt                                                                                                                                                                                                                                                                               >  2:184602/1‑301 (MQ=255)
                        tGGCGCTTGTTATGCTTCGGATCGGCACTGCAAATCACACGGATGACACAATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGc                                                                                                                                                                                                                                                                     <  1:694488/301‑1 (MQ=255)
                          gcgcTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAGGCCCGAACTTTCATTTTTACTCTCCGTACCTTCTCGGCCGACCATTTATCGGCCGAAGCATTTCAGGTTCGCCTTCTTCATTGCAGACTCATCCCGACTGTAC‑ATAATCAGAGTTTGCACTTGAGCCATCAAGTCCATAATACCGACAACAACGATAAGCAGTGAGGACCCACCATACTAGAACGGTACTTTCCTTGCATCACgaa                                                                                                                                                                                                                                                                    >  1:587251/1‑298 (MQ=255)
                          gcgcTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCAt                                                                                                                                                                                                                                                                   >  2:176948/1‑301 (MQ=255)
                             cTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTTAGCCATAAAGTCCCTAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCAGCACGCGTGaa                                                                                                                                                                                                                                                                >  1:22106/1‑301 (MQ=255)
                              tGTTTATGCTTCGGCTTGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAAc                                                                                                                                                                                                                                                               <  1:678962/301‑1 (MQ=255)
                              tGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAAc                                                                                                                                                                                                                                                               <  2:683414/301‑1 (MQ=255)
                              tGTTGATCCTTCGCGTCGGCCCTGCAAATCACACGGATGACCCCATCACGCTTAACGTTTTTGCAGTTACGGCATAATTTCTTGACGGAATCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGGCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAAc                                                                                                                                                                                                                                                               <  1:681724/301‑1 (MQ=255)
                               gTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCCGTGAGGTCCCACCGCAGTAGAACGGTACTTTCATTCAATCACGCCTGAAc                                                                                                                                                                                                                                                               >  1:488292/1‑300 (MQ=255)
                               gTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTTAGGTCCCACCGTAGTAg                                                                                                                                                                                                                                                                                             >  1:131672/1‑270 (MQ=255)
                               gTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAg                                                                                                                                                                                                                                                                                             <  2:131673/270‑1 (MQ=255)
                                    tGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAATGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCggg                                                                                                                                                                                                                                                         >  1:343920/1‑301 (MQ=255)
                                      cTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGACGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCCGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCAATTGAGCCATAAAGTCCATAATCACGAAAACAACGATAAGCAGTTAGGTCCCACCGAAGTAGAACGGTACTTTCCTTGCATCACGCATGAACTCCGGGaa                                                                                                                                                                                                                                                       >  1:855635/1‑300 (MQ=255)
                                         ctgctcggcACTGCAAATCCCACTGAGGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAg                                                                                                                                                                                                                                                    <  1:369692/299‑1 (MQ=255)
                                                     gCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGt                                                                                                                                                                                                                                        >  2:318786/1‑301 (MQ=255)
                                                     ctacaaatcacGGTTGACAGCATCCAGCTGAACGATTTTGCAGTTACGGCCTAATCTTCTGACGGAAGCACGTACTTTCATTTTTACTCCCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGt                                                                                                                                                                                                                                        <  1:361949/293‑1 (MQ=255)
                                                      cAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTCCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGCCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGt                                                                                                                                                                                                                                        <  2:855669/300‑1 (MQ=255)
                                                            ccacGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATAc                                                                                                                                                                                                                                 <  2:81202/300‑1 (MQ=255)
                                                            ccacGGATGAAACCACACCGCTTAACGATTTTGAAATTAAGGCATAATTCTTTGACGGAAGCACGAACTTTCATTTTTACTGTCCGTAACTTCTCGGGCGACTAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAAAAGCATACTGACTGGAC‑ATCATCAGAGTATGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGAGAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATAc                                                                                                                                                                                                                                 <  1:593723/300‑1 (MQ=255)
                                                            acacGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATAc                                                                                                                                                                                                                                 <  2:923278/301‑1 (MQ=255)
                                                            acacGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATAc                                                                                                                                                                                                                                 <  2:898339/301‑1 (MQ=255)
                                                            acacGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATAc                                                                                                                                                                                                                                 <  2:254115/301‑1 (MQ=255)
                                                            acacGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCAGGATCAGGCAGATAAAGGTAATATAc                                                                                                                                                                                                                                 <  1:176946/301‑1 (MQ=255)
                                                            acacGGATGACACCATCACACTTAATGATTTTGCAGTTACGGCATAATTGCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTATCTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCACTGCAGACTCCTACTGACTAGAC‑ATCATCAGAGTTTGCACTTGAGCCATAACGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCAGGATCAGGCAGATAAAGGTAATATAc                                                                                                                                                                                                                                 <  1:184599/301‑1 (MQ=255)
                                                            acacGGATGAAACCATCACCCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATAc                                                                                                                                                                                                                                 <  1:50597/301‑1 (MQ=255)
                                                            acacGAATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATAc                                                                                                                                                                                                                                 <  2:793529/301‑1 (MQ=255)
                                                              acGGATGACACTATCACGCTTAACCATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGAGACCAATTATCGGCCGTAGCATTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCAGAATCACGACAACAACGATAAGCAGTGAGGTCTCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGCATCAGGCAGATAAAGGTAATATACAg                                                                                                                                                                                                                               <  2:817249/301‑1 (MQ=255)
                                                                 gATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCAGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATAACGACAACAACGATAAGCAGAGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATAAAGCCGATAAAGGTAATATACAgag                                                                                                                                                                                                                             >  1:511419/1‑298 (MQ=255)
                                                                  aTGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAgcgc                                                                                                                                                                                                                            <  2:560313/300‑1 (MQ=255)
                                                                     acacCATCACGCTTAACGATTTTGCATTTACGGCATAATTTCTTGACTGAAGCCCGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCTGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGCTTGCACTTTAGCCATAAAGTCCATAATCACGACCACAACGATAAGCCGGACGGTCCCACCGCAGTAGACCGGTACTTTCATTGCATCAAGCATGAACTCCGGGAGCACGCAGAGACAGTTAATATCCAGCGCccc                                                                                                                                                                                                                         >  1:943116/1‑300 (MQ=255)
                                                                      cacCATTACGCTTAACGTTTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACAAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTg                                                                                                                                                                                                                                                                             <  1:963368/247‑1 (MQ=255)
                                                                      cacCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTg                                                                                                                                                                                                                                                                             >  2:963419/1‑247 (MQ=255)
                                                                      cacCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCacaaa                                                                                                                                                                                                                       >  2:554134/1‑301 (MQ=255)
                                                                        ccATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTACTATACAGCGCaccaac                                                                                                                                                                                                                      >  2:969449/1‑300 (MQ=255)
                                                                          aTCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAgcgc                                                                                                                                                                                                                            <  1:406499/292‑1 (MQ=255)
                                                                          aTCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAgcgc                                                                                                                                                                                                                            >  2:406506/1‑292 (MQ=255)
                                                                                cTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGCAGTAGAACGGTACTTTCATTACATCACGCATGAACCCCGCGATCAGGCAGATAAAGTTAATATACAGCGCCCCAACCAggtgcga                                                                                                                                                                                                             >  1:37852/1‑296 (MQ=255)
                                                                                        ttttGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCa                                                                                                                                                                                                     <  2:309532/301‑1 (MQ=255)
                                                                                             cAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTAc                                                                                                                                                                                                 <  2:93018/300‑1 (MQ=255)
                                                                                             cAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTAc                                                                                                                                                                                                 <  2:947195/300‑1 (MQ=255)
                                                                                             aggTTACGGCATAATTTCTTGACGGGAGCACGAACTTTCATTTTTACTCGCCGTAACTTATCCGGCGACCAATTTGCGGCCGTAGCCTTTCAGCTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTAc                                                                                                                                                                                                 <  1:224944/298‑1 (MQ=255)
                                                                                                         aaTTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATAc                                                                                                                                                                                    <  2:652251/301‑1 (MQ=255)
                                                                                                                                     tttttACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATACTCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGAGACTTTAATTGCATCACTCGTGAACTCCAGGAACAGGCAGATAAAGGTAATATACGGCGCACCAAACAGGGTCAGGCTGGTCATTACTTTAACCAAATACTTCCCCGttgtgtctacccgccaaatta                                                                                                                                                        >  1:881880/1‑282 (MQ=255)
                                                                                                                                     tttttACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAAtt                                                                                                                                                         >  2:254751/1‑300 (MQ=255)
                                                                                                                                     tttttACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGat                                                                                                                                                                                        >  1:34864/1‑269 (MQ=255)
                                                                                                                                     tttttACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGat                                                                                                                                                                                        <  2:34864/269‑1 (MQ=255)
                                                                                                                                            tctcCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTAc                                                                                                                                                 <  2:488300/301‑1 (MQ=255)
                                                                                                                                             ctcCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACa                                                                                                                                                >  1:4739/1‑301 (MQ=255)
                                                                                                                                             ctcCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCAGTACTTTATCGCTATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACa                                                                                                                                                >  2:1015852/1‑301 (MQ=255)
                                                                                                                                                     cTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGCGTTTGCACTTGCGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACTTGACTTTCATTGCAGCACGCATGACCTCCGGGATCAGGCAGATAATGGTAATATACAGCGCACCAACCAGGGACAGCCGGGTCGTTACTGTATCGTTATCCTCCGCCGTTTGCTCTACCGGTCGAATTCC‑GGTTCaaaaagcac                                                                                                                                        >  1:939470/1‑294 (MQ=255)
                                                                                                                                                     cTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGTAGATAAAGGTAATATAc                                                                                                                                                                                                                                 <  1:152323/212‑1 (MQ=255)
                                                                                                                                                                  aaaTTATCGGCCGTAGCCTTTCACGTTGGCCTTCTTCAATGCAGAATCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGACCATAATCACGACAACAACGATAAGCAGGGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAgg                                                                                                                           <  1:105636/300‑1 (MQ=255)
                                                                                                                                                                   aaTTATTGGCCGTCGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAA‑ATCAGCAGAGTTTGCACTTGATCCATAAAGTCCATAAACACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCAGGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGt                                                                                                                          <  1:872758/301‑1 (MQ=255)
                                                                                                                                                                    agtATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGGC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGt                                                                                                                          <  2:5090/298‑1 (MQ=255)
                                                                                                                                                                    aTTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATGCCTGGTACAAATGCACCGGCCTTCTACAGGtt                                                                                                                         >  1:801742/1‑301 (MQ=255)
                                                                                                                                                                           tcattatccTTTCATTTTCACCTTCTTCAAAGCAGACTCATACTGACAGGAC‑AGCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAAGGCTCCAACCttcttctttttacttt                                                                                                                    >  2:973617/8‑289 (MQ=255)
                                                                                                                                                                           gCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATctg                                                                                                                    >  2:817771/1‑299 (MQ=255)
                                                                                                                                                                                  ccTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATaa                                                                                                                                                                                                                                            <  2:677098/172‑1 (MQ=255)
                                                                                                                                                                                  ccTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATaa                                                                                                                                                                                                                                            >  1:677074/1‑172 (MQ=255)
                                                                                                                                                                                  ccTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTAAGGCGGGTCATTACTTTATCGATATACTTCGACGTTCGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTcc                                                                                                            >  1:475492/1‑299 (MQ=255)
                                                                                                                                                                                                   cttcAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGACAAcaacg                                                                                          >  2:353492/1‑297 (MQ=255)
                                                                                                                                                                                                   cttcAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCaa                                                                                           >  2:480538/1‑300 (MQ=255)
                                                                                                                                                                                                     tcAATGCAAACTAAAACTGA‑TGTAGAATCATCAGAGTTTTCAACTGGGTCATAAAGTCCATAATCACGACAACAACGATAAGACGTGAGGTACCACCGAAGTAGAACGGTACATTCATTGCATCACGCATGAACTCCGGGAGCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGAGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGTATTCCTGGTACAAATGCACCGTACTTCTTCAGGTTATCTGCTGTCTCACGCGGGTTGAAAACCAACGc                                                                                        <  1:653304/301‑1 (MQ=255)
                                                                                                                                                                                                             gACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGc                                                                                                                                                                                                                                                                     >  1:953475/1‑120 (MQ=255)
                                                                                                                                                                                                             gACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTaga                                                                                >  2:135179/1‑301 (MQ=255)
                                                                                                                                                                                                             gACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCACACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGAAGATAAAGGTAATATACAGCGCAACAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTaga                                                                                >  1:896902/1‑301 (MQ=255)
                                                                                                                                                                                                               cgcATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAAGCCCGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTagaag                                                                              <  1:480530/299‑1 (MQ=255)
                                                                                                                                                                                                               ccccaactgacttGAA‑ATCTCCAGAGTTTGCCCTGGAGCCATAAAGTCCAAAATAACGACAACAACGGTAAGCATTAAGGTCCCACCGAAGTAGAACTGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTGCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTagaag                                                                              <  1:1015800/296‑1 (MQ=255)
                                                                                                                                                                                                               cTCATCCTGAATGGAC‑ATCATCAGAGTGTGCACTTGCGACATAAAGTCCATAATCACGACAATAACTGTAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGTGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTagaag                                                                              <  1:554123/301‑1 (MQ=255)
                                                                                                                                                                                                               cTCATACTGACTGGAC‑AGCAACAGAGTTTGCCCTTGAGCCATCACGTCCATAATCACGACAACAACGAAAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCCCGCATGAACTCCGGGATCAGGCAGAGAAAGGTAAGATACAGCGCACCAACCAGGGTCAGGCGCGTCATTACTTTATCGATATACTTCTCCGTTTGCGCTCCCGGACGAAGTCCTTGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTagaag                                                                              <  1:295434/301‑1 (MQ=255)
                                                                                                                                                                                                                       gactgGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTCGAAGAAACagaa                                                                      >  1:1010545/1‑301 (MQ=255)
                                                                                                                                                                                                                       gactgGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCACTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTAc                                                                                                                                                                                                 >  1:101570/1‑178 (MQ=255)
                                                                                                                                                                                                                        actgGAC‑AGCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCAGTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACagaag                                                                     <  2:943161/301‑1 (MQ=255)
                                                                                                                                                                                                                                 tcatcaGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGc                                                                                        >  2:562439/1‑274 (MQ=255)
                                                                                                                                                                                                                                 tcatcaGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGc                                                                                        <  1:562428/274‑1 (MQ=255)
                                                                                                                                                                                                                                 tcatcaGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgatgat                                                             >  1:154298/1‑301 (MQ=255)
                                                                                                                                                                                                                                 tcatcaGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgatgaa                                                             >  1:466278/1‑300 (MQ=255)
                                                                                                                                                                                                                                    tcaGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGAGAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTGCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGc                                                          <  2:207907/301‑1 (MQ=255)
                                                                                                                                                                                                                                     caGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGc                                                          <  2:939512/300‑1 (MQ=255)
                                                                                                                                                                                                                                              cACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATag                                                <  2:475725/301‑1 (MQ=255)
                                                                                                                                                                                                                                                ttttaGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCATATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATagag                                              <  2:793168/297‑1 (MQ=255)
                                                                                                                                                                                                                                                tttGAGCAATAAAGTCCATAATAACGAAAACAACGCAAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACTCATGAACGCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATagag                                              <  1:273360/300‑1 (MQ=255)
                                                                                                                                                                                                                                                cTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATagag                                              <  2:4739/301‑1 (MQ=255)
                                                                                                                                                                                                                                                cTTAAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATagag                                              <  2:466285/301‑1 (MQ=255)
                                                                                                                                                                                                                                                      ccATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAacac                                        <  2:22106/301‑1 (MQ=255)
                                                                                                                                                                                                                                                      ccATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAacac                                        <  2:511428/301‑1 (MQ=255)
                                                                                                                                                                                                                                                        aTAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGCAGATGATTGCAGACGCATAGAGTAacaatt                                      >  2:705544/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                       aCGACAACAACGTTAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACGCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAg                       <  1:135178/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                          acaacaACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGc                     <  2:355924/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                acGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTgg                                                                                                                                                    <  2:333694/167‑1 (MQ=255)
                                                                                                                                                                                                                                                                                acGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTgg                                                                                                                                                    >  1:333688/1‑167 (MQ=255)
                                                                                                                                                                                                                                                                                acGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCaaaa              >  2:645382/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                 cGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATa             >  2:699877/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                   aTAAGCAGTGAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAACTACa           >  2:863622/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                            gAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAAc                                                                                              <  2:528227/209‑1 (MQ=255)
                                                                                                                                                                                                                                                                                            gAGGTCCCACCGTAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCAAGCGGGTTGAAAACCAACGCCGAGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGAGGTTGCCCAGGCTGCAAATACAGCGAAAttg  >  2:357845/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                
AAAAATATGCGAAAAAATCAGCCTTGGCGCTGTTTATGCTTCGGCTCGGCACTGCAAATCACACGGATGACACCATCACGCTTAACGATTTTGCAGTTACGGCATAATTTCTTGACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGAC‑ATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG  >  NC_000913/3442601‑3443184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A